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  4. Recombinant Mouse Inactive ubiquitin carboxyl-terminal hydrolase 44 (Usp44)

Recombinant Mouse Inactive ubiquitin carboxyl-terminal hydrolase 44 (Usp44)

  • 货号：
    CSB-YP805392MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP805392MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP805392MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP805392MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP805392MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Usp44
  • Uniprot No.：
  • 别名：
    Usp44; Ubiquitin carboxyl-terminal hydrolase 44; EC 3.4.19.12; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-711
  • 氨基酸序列
    MDRCKHVEQL QLAQGHSILD PQKWYCMVCN TTESIWACLS CSHVACGKYI QEHALKHFQE SSHPVAFEVN DMYAFCYLCN DYVLNDNAAG DLKSLRSTLS TIKSKKYPCV VPSDSVLHPV DAQDRVYSLL DGTQSLPGNE DPTCAALWHR RRVLMGKAFR TWFEQSAIGR KGQEPTQERM VAKREAKRRQ QQELEQQMKA ELESTPPRKS LRLQGSSEEA ATIEIVPVRA PPPPPASPAK DKAALPTSED RTFKKVSDSL IKRRPMVTPG VTGLRNLGNT CYMNSVLQVL SHLLIFRQCF LKLDLNQWLA VAASDKARSY KHSAVTEAAA QQMNEGQEKE KGFVCSRHSG LSSGLSGGAS KGRNMELIQP REPSSPYSSL CHELHILFQV MWSGEWALVS PFAMLHSVWR LIPAFRGYAQ QDAQEFLCEL LDKIQRELET TGTKLPALIP TSQRRLIEQV LNVVNNIFHG QFLSQVTCLA CDNKSDTIES FWDLSLEFPE RYQCSGKDAA SQPCLVTDML DKFTETEALE GKIYMCDHCN SKRRKFSSKS VVFTEAQKQL MICHLPQVLR LHLKRFRWSG RNNREKIGVH VVFEETLNME PYCCRETLNA LRPECFLYNL SAVVIHHGKG FGSGHYTAYC YNSEGGFWVH CNDSKLSMCT MEEVRKAQAY ILFYTQRVTE NGHSKLLPPE LLSNSQHPSK ETDASSNEVL S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.
  • 基因功能参考文献：
    1. USP44 inhibited chromosome segregation errors independent of its role in the mitotic checkpoint by regulating centrosome separation, positioning, and mitotic spindle geometry. PMID: 23187126
    2. These data are consistent with an important role for USP44 in regulating Cdc20-APC/C activity and suggest that high levels of this enzyme may contribute to the pathogenesis of T-cell leukemias. PMID: 21853124
  • 亚细胞定位：
    Nucleus. Note=Peaks in interphase, with relatively low levels maintained throughout mitosis.
  • 蛋白家族：
    Peptidase C19 family, USP44 subfamily
  • 组织特异性：
    Widely expressed. Highly expressed in lung, pancreas, skin, liver, stomach and intestine.
  • 数据库链接：

    KEGG: mmu:327799

    UniGene: Mm.491598