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  4. Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit beta (Ikbkb)

Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit beta (Ikbkb)

  • 货号：
    CSB-YP011572MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011572MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011572MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011572MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011572MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ikbkb; Ikkb; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; EC 2.7.11.10; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-757
  • 氨基酸序列
    MSWSPSLPTQ TCGAWEMKER LGTGGFGNVI RWHNQATGEQ IAIKQCRQEL SPKNRNRWCL EIQIMRRLNH PNVVAARDVP EGMQNLAPND LPLLAMEYCQ GGDLRRYLNQ FENCCGLREG AVLTLLSDIA SALRYLHENR IIHRDLKPEN IVLQQGEKRL IHKIIDLGYA KELDQGSLCT SFVGTLQYLA PELLEQQKYT VTVDYWSFGT LAFECITGFR PFLPNWQPVQ WHSKVRQKSE VDIVVSEDLN GAVKFSSSLP FPNNLNSVLA ERLEKWLQLM LMWHPRQRGT DPQYGPNGCF RALDDILNLK LVHVLNMVTG TVHTYPVTED ESLQSLKTRI QENTGILETD QELLQKAGLV LLPDKPATQC ISDSKTNEGL TLDMDLVFLL DNSKINYETQ ITPRPPPESV SCILQEPKRN LSFFQLRKVW GQVWHSIQTL KEDCNRLQQG QRAAMMSLLR NNSCLSKMKN AMASTAQQLK AKLDFFKTSI QIDLEKYKEQ TEFGITSDKL LLAWREMEQA VEQCGRENDV KHLVERMMAL QTDIVDLQRS PMGRKQGGTL DDLEEQAREL YRKLREKPRD QRTEGDSQEM VRLLLQAIQS FEKKVRVIYT QLSKTVVCKQ KALELLPKVE EVVSLMNEDE RTVVRLQEKR QKELWNLLKI ACSKVRGPVS GSPDSMNVSR LSHPGQLMSQ PSSACDSLPE SDKKSEELVA EAHALCSRLE SALQDTVKEQ DRSFTTLDWS WLQMEDEERC SLEQACD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.
  • 基因功能参考文献：
    1. The results demonstrate that the inhibition of miR-16 renders resistance to paclitaxel in vitro and in vivo by targeting IKBKB via NF-kappa B signaling pathway. PMID: 29935185
    2. Hepatic overexpression of N-terminal transactivation domain TAp63 induces liver steatosis through IKKbeta activation and the induction of ER stress. PMID: 28480888
    3. Astrocyte IKKbeta deletion following high-fat diet-but not before- reduced glucose intolerance and insulin resistance. Hypothalamic inflammation and astrocytosis were reduced in HFD-fed IKKbeta-AKO mice. PMID: 28377875
    4. IKKbeta is involved in membrane fusion, and serves as a critical protein kinase required for initial formation and the regulation of the CARMA1/MALT1/Bcl10/CBM complex in platelets. PMID: 29649481
    5. NF-kappaB is the transcription factor in regulating many proinflammatory cytokine genes. PMID: 29595073
    6. p55TNFR-IKK2-Ripk3 signalling orchestrates arthritogenic and death responses in synovial fibroblasts PMID: 29434332
    7. Pulmonary inflammation due to lung-specific overexpression of IKK2ca was sufficient to increase circulating TNFalpha and IL-6 levels, adipose expression of Tnfalpha and Il-6 mRNA, aortic endothelial dysfunction, and systemic insulin resistance. PMID: 29036520
    8. prolonged IKKbeta inhibition decimates circulating Tregs and improves Cytotoxic t lymphocytes responses when commenced after tumor vaccination, indicating that IKKbeta represents a druggable checkpoint. PMID: 29045828
    9. Results reveal that IKKbeta activity prevents skin tumor development, and shed light on the complex nature of IKKbeta effects on cancer progression, as IKKbeta can both promote and prevent carcinogenesis depending on the cell type or molecular context. PMID: 28584022
    10. IKK2 activation in astrocytes initiates selective non-cell-autonomous neurodegeneration PMID: 28193238
    11. Chronic overnutrition and astrocytic IKKbeta/NF-kappaB upregulation similarly impaired astrocytic plasticity, leading to sustained shortening of high-order processes. In physiology, astrocytic IKKbeta/NF-kappaB upregulation resulted in early-onset effects, including glucose intolerance and blood pressure rise, and late-onset effects, including body weight and fat gain. PMID: 28467927
    12. Expression of both wild-type and catalytically-inactive Y7F mutant GSTP significantly attenuated LPS- or IKKbeta-induced production of GM-CSF. These studies indicate a complex role for GSTP in modulating NF-kappaB, which may involve S-glutathionylation of IKK proteins, and interaction with NF-kappaB family members. PMID: 27058114
    13. Myeloid ikkbeta gene deletion significantly reduced the percentage of CD4(+)/IFN-gamma(+) (Th1) and CD4(+)/IL-17(+) (Th17) cells PMID: 27450563
    14. Pharmacological and genetic inhibition of IKKbeta decreased voluntary ethanol consumption, providing initial support for IKKbeta as a potential therapeutic target for alcohol abuse. PMID: 27822501
    15. Myeloid IKKbeta deficiency prevents Western diet-induced obesity and visceral adiposity in females but not in males, and attenuates hyperglycemia, global insulin resistance, and non-alcoholic steatohepatitis in both genders. In contrast, all metabolic sequela including non-alcoholic steatohepatitis are aggravated by hepatocyte IKKbeta deficiency (IkbkbDeltahep) in male but not female mice. PMID: 28797077
    16. lymphocyte-specific deletion of IKK2 or NEMO aggravated kidney injury after ischemia-reperfusion injury, and, in both conditions, the percentage of Th17 cells was increased. PMID: 27582100
    17. data clearly demonstrate that KLHL21 negatively regulates TNFalpha-activated NF-kappaB signaling via targeting IKKbeta, providing new insight into the mechanisms underlying NF-kappaB regulation in cells. PMID: 27387502
    18. Study used three experimental approaches to enhance the IKKbeta activity in the liver of obese mice and observed increased XBP1s activity, reduced endoplasmic reticulum stress, and a significant improvement in insulin sensitivity and consequently in glucose homeostasis. PMID: 27814504
    19. Mutation of cysteine 46 in IKK-beta increases inflammatory responses. PMID: 26378659
    20. signaling in keratocytes required in corneal wound healing PMID: 26987064
    21. TAK1 and IKK2 are novel mediators of SCF-induced signaling and potential targets for c-Kit-driven diseases. PMID: 26353931
    22. MyD88s is positively regulated by IKKbeta and CREB and negatively regulated by ERK1/2 signaling pathways. PMID: 26669856
    23. these results highlight an unexpected tumor suppressive function of IKKbeta/NF-kappaB in cancer-associated fibroblasts. PMID: 26621452
    24. These results reveal a hitherto unknown role of mesenchymal IKKbeta in driving inflammation and enabling carcinogenesis in the intestine. PMID: 26621453
    25. results provide evidence for a previously unrecognized role of IKKbeta in the regulation of the growth plate that is mediated through stimulation-independent downregulation of MCP-5 in the perichondrium. PMID: 25526093
    26. IKKbeta was required in both for modification of their hippocampus-dependent appetitive, not consummatory behavior. PMID: 25297800
    27. The role of IKK2 in heart injury induced by ambient fine particulate matter is reported. PMID: 25968264
    28. In models of diffuse large B-cell lymphoma (DLBCL), the cytotoxicity of LY2409881 (novel inhibitor of IKK2) correlated with the overall activation status of NF-kappaB. PMID: 25355930
    29. IKKb activates the expression of proinflammatory mediators, and in adipocytes, IKKb signaling also induces the expression of the anti-inflammatory cytokine IL-13. PMID: 25466256
    30. These experiments reveal both overlapping and nonredundant effects of JNK- and IKK-dependent signaling in AgRP neurons. PMID: 25456138
    31. Results indicate cell proliferation defect in I-kappa B (IkappaBNS)-deficient Th17 cells. PMID: 25694610
    32. Constitutive activation of IKK2/NF-kappaB impairs osteogenesis and skeletal development. PMID: 24618907
    33. Loss of IKK complex components impairs the mast cell late-phase response to allergen. Constitutive IKK2 activity enhances the mast cell late-phase cytokine response. PMID: 25176657
    34. IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420
    35. RelB serine 472 phosphorylation status controls MMP3 expression and promigration activity downstream of TNF receptors. PMID: 25267645
    36. The antioxidant catalase counteracts against high fat diet-induced cardiac geometric and functional anomalies possibly via an IKKbeta-AMPK-dependent restoration of myocardial autophagy. PMID: 24993069
    37. Central inhibition of IKKbeta prevents PM2.5 mediated peripheral inflammation and exaggeration of type II diabetes. PMID: 25358444
    38. miR-339-5p inhibits expression of the pro-inflammatory factors in alcohol-induced brain inflammation through the NF-kappaB pathway by abolishing IKK-beta and IKK-epsilon activity. PMID: 25172655
    39. mice with continuous IKKbeta signaling in myeloid-lineage cells (IKKbeta(CA)) exhibited enhanced antitumor immunity and reduced melanoma outgrowth PMID: 25336190
    40. the requirement of IKKbeta for VEEV replication PMID: 24586253
    41. these findings reveal a previously unappreciated specificity of the IKKbeta/NF-kappaB signaling axis in regulation of antimicrobial responses by different classes of pattern recognition receptors PMID: 25057006
    42. Deficiency of IKKbeta in myeloid cells reduces Abeta load in aged APP-transgenic mouse brains. PMID: 25253847
    43. IKKbeta in intestinal epithelial cells regulates allergen-specific IgA and allergic inflammation at distant mucosal sites. PMID: 23839064
    44. IKKB regulates gene expression by transcription factors that are not P65 or rel-c dependent during C26 cancer cachexia. PMID: 24489962
    45. Data suggest that TAK1 (MAP kinase kinase kinase 7) and IKKbeta phosphorylate different serines of IKKbeta; TAK1-catalyzed phosphorylation of IKKbeta at Ser177 is priming event that enables IKKbeta to activate itself by phosphorylating Ser181. PMID: 24911653
    46. IKKbeta and IKKalpha are simultaneously essential for cell migration in response to CXCL12 alone. PMID: 24747690
    47. persistent IKK2/NF-kappaB signalling in forebrain neurons does not induce overall neuroinflammation, but elicits a selective inflammatory response in the dentate gyrus accompanied by decreased neuronal survival and impaired learning and memory PMID: 24119288
    48. IKKbeta plays a pivotal role in linking vascular inflammation to atherosclerosis and adipose tissue development, thus providing evidence for using appropriate IKKbeta inhibitors in the treatment of obesity and metabolic disorders. PMID: 24799533
    49. linear chain-mediated activation of IKK2 involved homotypic interaction of the IKK2 kinase domain PMID: 24469399
    50. give the first direct evidence that beta-cell-specific IKK2/NF-kappaB activation is a potential trigger of immune-mediated diabetes PMID: 24296718

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane raft.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
  • 组织特异性：
    Detected in heart (at protein level). Expressed in liver, kidney and spleen.
  • 数据库链接：

    KEGG: mmu:16150

    STRING: 10090.ENSMUSP00000033939

    UniGene: Mm.277886