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  4. Recombinant Mouse Inositol polyphosphate multikinase (Ipmk)

Recombinant Mouse Inositol polyphosphate multikinase (Ipmk)

  • 货号：
    CSB-YP011774MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011774MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011774MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011774MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011774MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ipmk; Impk; Ipk2; Inositol polyphosphate multikinase; EC 2.7.1.140; EC 2.7.1.151; EC 2.7.1.153; Inositol 1,3,4,6-tetrakisphosphate 5-kinase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-396
  • 氨基酸序列
    AAEPPALRL RPPGSTGDSP PVPRLLGGCV PLSHQVAGHM YGKDKVGILQ HPDGTVLKQL QPPPRGPREL EFYTMVYAAD CADAVLLELR KHLPKYYGVW SPPTAPNDVY LKLEDVTHKF NKPCIMDVKI GRKSYDPFAS SEKIQQQVSK YPLMEEIGFL VLGMRVYHLH SDSYETQNQH YGRGLTKETL KEGVSKFFHN GFCLRKDAIA ASIQKVEKIL QWFENQKQLN FYASSLLFVY EGSSQPATTK ANDRTLAGRF LSKGPLTDAD GLECNNNFHL FGAPPNGMSV GKSLSKAYSR HRKLYAKKHQ SQTSLKVETL EQDNGWRSMS QEHLNGNVLA QLEKVFYHLP AGRPEIPEAE VRMIDFAHVF PSNTVDEGYV YGLKHLIAVL RSILDS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis. Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).
  • 基因功能参考文献：
    1. these findings identify IPMK as a key determinant of TRAF6 stability and elucidate the physiological function of IPMK in TLR-induced innate immunity. PMID: 28439546
    2. Data show that phospholipase C (PLC)-beta1 (PLC-beta1) overexpression determines an increase in beta-catenin translocation and that PLC-beta1, inositol polyphosphate multikinase (IPMK) and beta-catenin are mediators of the same signaling pathway. PMID: 27563828
    3. a severe loss of IPMK in the striatum of Huntington disease patients and in several cellular and animal models of the disease, is reported. PMID: 26195796
    4. metformin-mediated activation of AMP-Activated Protein Kinases was impaired in the absence of IPMK. PMID: 24877601
    5. Inositol polyphosphate multikinase is a coactivator for serum response factor-dependent induction of immediate early genes. PMID: 24248338
    6. Knockdown of expression and/or inhibition of function of phospholipase Cgamma1, inositol polyphosphate multikinase, which generates inositol 1,3,4,5-tetrakisphosphate (InsP) and InsP, and inositol hexakisphosphate kinase 1/2 PMID: 23322705
    7. Inositol polyphosphate multikinase is a transcriptional coactivator required for immediate early gene induction. PMID: 24043835
    8. IPMK regulates glucose signaling to AMPK in a pathway whereby glucose activates phosphorylation of IPMK at tyrosine 174 enabling the enzyme to bind to AMPK and regulate its activation. PMID: 22203993
    9. Data show that in MEFs lacking IPMK, synthesis of IP5 and IP7 is abolished, but IP6 formation is reduced about 90%. PMID: 21220345
    10. Inositol polyphosphate multikinase (Ipk2) plays a major role in the synthesis of inositol polyphosphate messengers derived from inositol 1,4,5-trisphosphate in embryogenesis and normal development. PMID: 15939867

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Inositol phosphokinase (IPK) family
  • 数据库链接：

    KEGG: mmu:69718

    STRING: 10090.ENSMUSP00000078240

    UniGene: Mm.245867