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  4. Recombinant Mouse Insulin-2 (Ins2)

Recombinant Mouse Insulin-2 (Ins2)

  • 中文名称：
    小鼠Ins2重组蛋白
  • 货号：
    CSB-EP360644MO
  • 规格：
    ￥2328
  • 图片：
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360644MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Ins2.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360644MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Ins2.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Ins2
  • Uniprot No.：
  • 别名：
    Ins2; Ins-2; Insulin-2 [Cleaved into: Insulin-2 B chain; Insulin-2 A chain]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    16.9 kDa
  • 表达区域：
    25-110aa
  • 氨基酸序列
    FVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKRGIVDQCCTSICSLYQLENYCN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 基因功能参考文献：
    1. High INS2 expression is associated with weight gain and obesity. PMID: 29122848
    2. Data (including data from studies in knockout mice) suggest that Ins2 is involved in impaired nociception/diabetic neuropathy; here, mice heterozygous for mutant Ins2 exhibit (a) significant loss of intra-epidermal nerve fibers, (b) markedly reduced responsiveness to heat in dorsal root ganglion neurons, and (c) mostly unchanged function of cold-sensitive neurons; such mice become diabetic soon after weaning. PMID: 29875100
    3. These results suggest that PABP interacts with HuD in basal glucose conditions making translation inhibitory complex, however upon glucose stimulation this association is affected and PABP is acted upon by PDI resulting in stimulation of insulin translation. PMID: 29590218
    4. cTAGE5 deletion in pancreatic beta cells impairs proinsulin trafficking and insulin biogenesis in mice. PMID: 29133483
    5. report that EndMTs occur in the diabetic endothelium of Ins2Akita/wt mouse, and show that induction of sex determining region Y-box 2 (Sox2) is a mediator of excess BMP signaling that results in activation of EndMTs and increased vascular calcification PMID: 27936229
    6. Transplantation of transduced hematopoietic stem cells (HSCs) expressing proinsulin II prevents diabetes development. PMID: 26784909
    7. Wnt3a increased the expression of NeuroD1 and Ins2 in the hypothalamus. PMID: 26956881
    8. have characterized the distinctive sex-specific phenotypes exhibited by the ApoE(-/-):Ins2(+/Akita) mouse model and present evidence for the action of sex hormones on pancreatic beta-cell function PMID: 26597883
    9. Data indicate that insulin/incomplete Freund's adjuvant (IFA) does not prevent but induces diabetes in RIP-CD80GP transgenic mice. PMID: 24387268
    10. RORalpha is a transcriptional activator of insulin. PMID: 24583012
    11. Mice deficient in coinhibitory PD-L1 or PD-1 molecules (PD-L1(-/-) and PD-1(-/-) mice), were used to study induction of preproinsulin (ppins)-specific CD8 T-cell responses and experimental autoimmune diabetes. PMID: 23977133
    12. Akita mouse has a mutation in Ins2 and is a model of the effects of maternal and paternal hyperglycemia in wildtype offspring PMID: 23209676
    13. Cortical bone was affected in STZ but not Ins2(+/-) mice. PMID: 22886636
    14. proSAAS as a novel down-regulated target of Pax6 PMID: 23056534
    15. Leptin administration improves dyslipidemia and reduces atherosclerosis in type 1 diabetic Ins2(+/Akita):apoE(-/-) mice. PMID: 23099119
    16. Ins2(Akita) mouse is a good model for later-onset DR, modeling both early and some late disease signs. PMID: 23221078
    17. The Akita(ins2) type 1 diabetic model is protected against systolic failure due to increased NCX1 expression via a CXCR4/NF-kappaB pathway. PMID: 22610174
    18. Integrin alpha1KOAkitaKO Balb/c mouse represents a promising model presenting with most features of human diabetic nephropathy. PMID: 22297672
    19. these results indicate that CaMKIIdelta2 downregulates insulin gene expression by Ser142 phosphorylation of CREB and reducing binding of CREB to CBP. PMID: 22554507
    20. HuD controls insulin II translation PMID: 22387028
    21. Data show that TMED6 mRNA is highly and selectively expressed in pancreas,and knockdown of TMED6 gene expression in Min6 beta cells decreased insulin secretion. PMID: 22129529
    22. The results indicated that intraislet ghrelin does not play a major role in the regulation of insulin secretion in vivo. PMID: 22114024
    23. Data suggest that high glucose mediates the recruitment of p300, CBP, PCAF, and GCN5 to the insulin promoter and that all four histone acetyltransferase (HATs) are important for insulin gene expression. PMID: 21774670
    24. Raf-1 specifically and acutely regulates insulin 2 mRNA via negative action on Foxo1 PMID: 21817126
    25. The findings demonstrate that the perturbation of proinsulin homeostasis results in defects in the subsequent conversion process of proinsulin and is a contributor to the occurrence of disproportionate hyperproinsulinemia in diabetes. PMID: 21723250
    26. Exaggerated hypercholesterolemia and atherosclerosis in spontaneously diabetic Ins2(+/Akita):apoE(-/-) mice may be attributable to impaired lipoprotein clearance in the setting of diminished expression of LSR. PMID: 21447785
    27. The transcriptional programs in both endoneurial and neuronal compartments of the peripheral nerve are relatively resistant to the onset of hyperglycemia and hypoinsulinemia in Ins2 mice. PMID: 20520806
    28. Alternative splicing of insulin mRNA in mice could result in an additional level of regulation in insulin biosynthesis. PMID: 20153322
    29. These studies identify strong effects of genetic background to modify the renal phenotype associated with the Ins2(C96Y) mutation. PMID: 20042456
    30. Type 1 diabetic cardiomyopathy in the Akita (Ins2WT/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function. PMID: 19801494
    31. The interval between Ins2 and Ascl2 is dispensable for imprinting centre function in the mouse model of Beckwith-Wiedemann Syndrome. PMID: 19684026
    32. mutated in iddm in mice PMID: 11981430
    33. findings suggest that the organelle dysfunction resulting from the intracellular accumulation of misfolded proinsulin 2 is primarily responsible for the defect of coexisting wild-type insulin secretion in Akita beta-cells PMID: 12540615
    34. Transcriptional up-regulation of the remaining functional insulin gene in Ins2-/- mice could potentially contribute to beta-cell adaptation. PMID: 12745665
    35. Wild-type preproinsulin-2-expressing pancreatic islets transplanted in preproinsulin-2-deficient mice elicit a mononuclear cell infiltration and insulin antibodies; graft infiltration is further increased by immunization with preproinsulin-2 peptides. PMID: 14688305
    36. A missense mutation of the insulin 2 gene (Cys96Tyr) disrupts 1 of the 2 interchain disulfide bonds resulting in accumulation of misfolded protein in pre-Golgi intermediates. PMID: 15033933
    37. platelet-derived growth factor-induced cell proliferation is inhibited by insulin PMID: 15525682
    38. Pancreatic beta-cells that glucose stimulates the recruitment of ribosome-associated proinsulin mRNA, located in the cytoplasm, to the ER, the site of proinsulin synthesis; this plays an important role in glucose-stimulated proinsulin synthesis. PMID: 15972000
    39. Proinsulin-2 gene expression by radioresistant thymic epithelial cells is involved in the induction of self-tolerance, and additional factors are required to induce islet abnormalities. PMID: 16785498
    40. Munich Ins2 mutant mice are a valuable model to study the mechanisms of beta-cell dysfunction and death during development of diabetes. PMID: 17303807
    41. diabetes causes fusion between Proins-P bone marrow-derived cells and hepatocytes PMID: 17360472
    42. Cre recombinase is controlled by a short fragment of the rat insulin II gene promoter PMID: 17533574
    43. The data underline a direct role for Aire in tissue-restricted antigens expression and suggest that modulation of Aire has a potential to control central tolerance and autoimmunity. PMID: 17599412
    44. misfolded proinsulin causes enlargement of pre-Golgi intermediates which indicates their involvement in protein quality control. PMID: 17647009
    45. Nonobese, insulin-deficient Ins2(Akita) mice develop type 2 diabetes phenotypes including peripheral and hepatic insulin resistance and cardiac remodeling. PMID: 17911348
    46. Insulin II is a direct target of RHOX5. PMID: 18184911
    47. Arterial superoxide generated from diverse sources may potentiate the contractions of carotid arteries in Ins2(Akita) diabetic mice. PMID: 18788099
    48. Mutant huntingtin disrupts intracellular transport and insulin secretion by interacting with tubulin beta 5. PMID: 19628478
    49. Ins2 expression by NOD bone marrow-derived cells did not delay diabetes development in NOD-Ins2-/- mice. PMID: 19874548

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: mmu:16334

    STRING: 10090.ENSMUSP00000000220

    UniGene: Mm.4946