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  4. Recombinant Mouse Insulin gene enhancer protein ISL-1 (Isl1)

Recombinant Mouse Insulin gene enhancer protein ISL-1 (Isl1)

  • 货号：
    CSB-YP011846MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011846MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011846MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011846MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011846MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Isl1; Insulin gene enhancer protein ISL-1; Islet-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-349
  • 氨基酸序列
    MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart.
  • 基因功能参考文献：
    1. Mouse cardiac progenitor cells fate transitions are associated with distinct open chromatin states critically depending on Isl1 and Nkx2-5 expression. PMID: 30451828
    2. ISL1 is a major susceptibility gene for bladder exstrophy and a regulator of urinary tract development. PMID: 28176844
    3. ISL1 plays a central role in proper patterning of stem cell-derived enamel in the incisor and indicate that this factor is an important upstream regulator of signaling pathways during tooth development and renewal. PMID: 28650075
    4. Both ISL1-LHX3 and ISL1-LHX4 bound to the Slit2 enhancer. PMID: 27819291
    5. results support a model in which Gata6 contributes to repression of Isl1 expression in the anterior of nascent hindlimb buds. PMID: 29197504
    6. The results demonstrated that Islet1 upregulated expression of general control of amino acid biosynthesis protein 5 (Gcn5) and enhanced the binding of Gcn5 to the promoters of GATA binding protein 4 (GATA4) and NK2 homeobox 5 (Nkx2.5). In addition, Islet-1 downregulated DNA methyltransferase (DNMT)1 expression and reduced its binding to the GATA4 promoter. PMID: 28447752
    7. Isl1beta is a key additional transcriptional factor for advancing the generation of insulin-producing cells in the liver in combination with PDA (Pdx1, Neurod1, and Mafa). PMID: 29220426
    8. Overexpression of Islet1 affects the development and function of the cerebello-vestibular system, resulting in hyperactivity. PMID: 26843111
    9. Results show that specific genomic loci associated with Islet-1 (Isl1) , LIM-homeobox 3 (Lhx3), and LMO4 genes recruit the Isl1-Lhx3 complex to activate the transcription of Isl1, Lhx3, and Lmo4. PMID: 28451636
    10. these results uncover a novel role for VEGF in controlling proper allocation of Isl1(+) cardiac progenitors to their respective descending lineages PMID: 27794491
    11. CITED2 and ISL1 proteins interact physically and cooperate to promote embryonic stem cell differentiation toward cardiomyocytes. PMID: 27818139
    12. Together, the s show the antagonistic regulation of the alpha-enhancer activity by Pax6 and the LIM protein complex is necessary for the establishment of an inner retinal circuitry, which controls visual adaptation. PMID: 28139974
    13. results identify Isl1 as a crucial transcription factor that plays essential roles in the gene regulatory program directing development of multiple arcuate neuronal subpopulations. PMID: 27578785
    14. Expression of motor neuron effector genes is maintained by transient enhancers bound by Isl1/Lhx3 in nascent motor neurons and Isl1/Onecut1 in maturing hypaxial motor neurons. PMID: 27939581
    15. These data reveal functional roles for an ISLET1-dependent network integrating beta-catenin/SHH signals in mesenchymal cell survival and outgrowth of the mandible during development. PMID: 28069742
    16. ISL1 and JMJD3 partner to alter the cardiac epigenome, instructing gene expression changes that drive cardiac differentiation. PMID: 27105846
    17. Pax2-regulated ISL1 overexpression increases the embryonic ISL1(+) domain and induces accelerated nerve fiber extension and branching in E12.5 embryos. PMID: 25990412
    18. These results show that ISL1 is necessary for maximal thyrotrope response to hypothyroidism, in addition to its role in development of Rathke's pouch. PMID: 26296153
    19. the Isl1/Ldb1 complex orchestrates a network for heart-specific transcriptional regulation and coordination in three-dimensional space during cardiogenesis. PMID: 26321200
    20. All E1 sequences from lamprey to mouse responded equally well to Phox2a and the Isl1-Lhx3 complex. Conversely, E2, the enhancer for limb-innervating motor neurons, was only found in tetrapod animals PMID: 26447474
    21. Isl1 alternative splicing varies across different retinal cell types. Two isoforms of Isl1 differ functionally as transcriptional activators. PMID: 25752730
    22. this study gives new insights into transcriptional regulating mechanisms of Wnt-mediated Isl1 expression during cardiomyocyte differentiation. PMID: 24610003
    23. results demonstrate that ISL1 regulates approximately one-third of SAN-specific genes, indicate that a combination of ISL1 and other SAN transcription factors could be utilized to generate pacemaker cells PMID: 26193633
    24. lack both Isl1 and Isl2, large numbers of motor neurons exited the neural tube, even prior to the appearance of boundary cap cells at the ventral exit points PMID: 25843547
    25. Data indicate that LIM-homeodomain transcription factor Islet 1 is necessary for hypothalamic proopiomelanocortin gene (Pomc) expression during embryogenesis. PMID: 25825735
    26. Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate in ATOH7 deficient mice PMID: 25775587
    27. The transcription factor Islet-1 is a positive transcriptional regulator of the cardiac pacemaker cell gene expression program in the developing sinoatrial node. PMID: 25623957
    28. KISS-54 influences Isl-1 expression and insulin secretion through the protein kinase C-ERK1/2 pathway. PMID: 24956377
    29. The transcription factors Sox2 and Isl1 physically interact and cooperatively function to sustain Atoh1 expression in developing Merkel cells. PMID: 25468937
    30. Study demonstrates a sequential expression order of NEUROD1>ISL1>POU4F1>POU4F2 during the inner ear neurogenesis. PMID: 24709358
    31. Expression pattern of Isl1 and its co-factor Ldb1, were analyzed in small intestine. PMID: 24755910
    32. Isl-1 is essential for postnatal beta-cell function, directly regulates Pdx1 and Slc2a2, and has a mature beta-cell cistrome distinct from that of pancreatic endocrine progenitors. PMID: 25028525
    33. Isl1 is required for the specification of a subset of enteroendocrine cells leading to lipid malabsorption in neonatal mice and the loss of the incretin response in adults. PMID: 25214396
    34. Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells. PMID: 24643061
    35. Results suggest that ISL1 is intimately involved in early regulation of adipogenesis, modulating PPARgamma expression and activity via BMP4-dependent and -independent mechanisms. PMID: 25047837
    36. This study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. PMID: 24674670
    37. ISL1 is expresseed in multiple tissues during mouse development. PMID: 23906961
    38. The data suggested that Islet-1 might function as an assistant factor that was involved in the regulation of histone acetylation and Mef2c expression via assisting p300 on specifically targeting the promoter of Mef2c. PMID: 24147056
    39. the Isl1-beta-catenin pathway regulates skeletal development of the hindlimb and lower jaw through discrete populations of cells that give rise to Shh-expressing posterior hindlimb mesenchyme and Fgf8-expressing mandibular epithelium. PMID: 24424161
    40. Islet-1 has a role in promoting striatonigral and repressing striatopallidal genetic programs to specify striatonigral cell identity PMID: 24351932
    41. Conditional inactivation of Isl1 in the telencephalon resulted in a smaller striatum with fewer striatonigral neurons and reduced projections to the substantia nigra. PMID: 24082127
    42. This study demonistrated that hair cell-specific Isl1 expression can promote hair cell survival and therefore minimize the hearing impairment that normally occurs with aging and/or acoustic overexposure. PMID: 24048839
    43. Data characterized an intramolecular interaction between the LIM domains and LIM homeobox 3 (Lhx3)-binding domain in Isl1. PMID: 23750000
    44. Isl1 is essential for proper development of the kidney and ureter by repressing the aberrant formation of the ureteric bud PMID: 23641053
    45. In Islet-1 deficient mouse embryos the numbers of chromaffin cells were only mildly reduced, in contrast to that of sympathetic neurons PMID: 23648511
    46. Our findings demonstrate a novel epistatic relationship between Shox2 and Isl1 in the heart with important developmental consequences for sinoatrial node formation and heart beat. PMID: 23455426
    47. LIM-homeodomain transcription factor Isl-1 mediates the effect of leptin on insulin secretion in mice. PMID: 23504315
    48. spinal microcircuits that integrate cutaneous feedback crucial for paw grip rely on the intermediary role of isl1 expression interneurons. PMID: 23583114
    49. Isl1 and the Ldb co-regulators of transcription are essential early determinants of mouse limb development PMID: 22411555
    50. Data suggest that Ldb1 is critical transcriptional coregulator during pancreatic islet alpha-, beta-, and delta-cell development through Isl1-dependent and potentially Isl1-independent control mechanisms. PMID: 23193182

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  • 亚细胞定位：
    [Isoform 1]: Nucleus.; [Isoform 2]: Nucleus.
  • 数据库链接：

    KEGG: mmu:16392

    STRING: 10090.ENSMUSP00000044879

    UniGene: Mm.42242