Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Insulin-like growth factor 1 receptor (Igf1r), partial

Recombinant Mouse Insulin-like growth factor 1 receptor (Igf1r), partial

  • 货号：
    CSB-YP011087MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011087MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011087MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011087MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011087MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Igf1rInsulin-like growth factor 1 receptor; EC 2.7.10.1; Insulin-like growth factor I receptor; IGF-I receptor; CD antigen CD221) [Cleaved into: Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R. When present in a hybrid receptor with INSR, binds IGF1.
  • 基因功能参考文献：
    1. IGF-1R signalling contributes to T cell dependent inflammation in arthritis. Inhibition of IGF-1R on the level of insulin receptor substrates alleviates arthritis by restricting IL6-dependent formation of Th17 cells and may open for new treatment strategies in rheumatoid arthritis. PMID: 28583713
    2. These results suggested that appropriate WBV may delay the progression of AS, which was associated with acutely elevated serum IGF-1 and lower levels of IGF-1 and IL-6 in the aorta for long-term treatment. PMID: 29707570
    3. Expression of IGF-1 and LEPR indicates a relevant role in androgenic features reversion present in PCOS, hormonal equilibrium, body weight regulation, and glucose metabolism, therefore, under phenotype obesity and infertility regulation in this model. PMID: 29430464
    4. Data identify central IGF-1R as a mediator of the integration of nutrient and temperature homeostasis. They also show that calorie restriction, IGF-1R signaling, and body temperature, three of the main regulators of metabolism, aging, and longevity, are components of the same pathway. PMID: 28827363
    5. Data (including data from studies in knockout mice) suggest double knockout (DKO) mice lacking Insr and Igf1r exhibit obesity with insulin resistance and increased adiposity; on high-fat diet, DKO mice exhibit metabolic syndrome. (Insr = insulin receptor; Igf1r = insulin-like growth factor I receptor) PMID: 29040448
    6. results demonstrate for the first time that Igf1r is important in acute asthma pathobiology and resolution of HDM-induced inflammation PMID: 29272313
    7. This study found that expressions of Cav3.2 and IGF-1R, and their colocalization were not increased in DRGs of mice following axotomy. In addition, Cav3.2 or IGF-1R subpopulation neurons did not acquire significant switch in expression phenotype after sciatic nerve axotomy. PMID: 27571431
    8. The present observations indicate that IGF-IR regulates glucose transporter 1 activity in astrocytes. PMID: 27462832
    9. Epithelial IGF1R is dispensable for IGF2-mediated enhanced intestinal adaptation after small bowel resection in retinoblastoma-deficient mice. PMID: 28343662
    10. these data suggest that the decreased mammary gland tumor latency time caused by chronic IGF1R activation is related to modulation of tumor progression rather than increased tumor initiation. PMID: 28173837
    11. data show that the GSK3B-FOXO3 pathway is activated after partial hepatectomy, and this may be one of the mechanisms that lead to upregulation of hepatic IGF1R after partial hepatectomy. PMID: 28952285
    12. Data show that tumors lacking PSMA had less than half the abundance of type 1 insulin-like growth factor receptor (IGF-1R), less activity in the survival pathway mediated by PI3K-AKT signaling, and more activity in the proliferative pathway mediated by MAPK-ERK1/2 signaling. PMID: 28292957
    13. let-7g regulates autophagy in mouse granulosa cells by targeting insulin-like growth factor 1 receptor and downregulating protein kinase B/mammalian target of rapamycin signaling PMID: 27417237
    14. VEGF and IGF1 were critical factors for the spontaneous cardiac differentiation of BATDCs, and MEK/ERK signaling was involved in the role of VEGF and IGF1. PMID: 27870972
    15. miR-223/IGF-IR signalling pathway is regulated in parallel in both the lung vasculature and the right ventricle under conditions of hypoxia induced pulmonary hypertension. PMID: 27013635
    16. IGF1R has a crucial role in granulosa cells function and, consequently, in female fertility. PMID: 28407051
    17. Ubiquitous inducible IGF-1R knockout (UBIKOR) mice were strikingly lean with coordinate changes in body composition and organ size. They were insulin resistant but preserved physiological energy expenditure and displayed enhanced fasting metabolic flexibility. PMID: 28881863
    18. the overlap of IR and IGF1R signaling is critical to the regulation of muscle protein turnover, and this regulation depends on suppression of FoxO-regulated, autophagy-mediated protein degradation PMID: 27525440
    19. SUMOylation of IGF1R affects upstream mechanisms that control and coordinate expression of cell cycle regulators to increase cell cycle progression and proliferation. PMID: 28112398
    20. findings demonstrated that significant resistance of the brain to amyloid-beta can be achieved lifelong by suppressing neuronal IGF1R. PMID: 28595357
    21. Results found that glioblastoma tumors resistant to PDGFR inhibition required the expression and activation of the insulin receptor (IR)/insulin growth-like factor receptor (IGF1R) for tumor cell proliferation and survival. PMID: 28138037
    22. Conditional deletion of synaptotagmin-10, the Ca(2+) sensor for IGF1 secretion from mitral cells, or deletion of IGF1 receptor in the olfactory bulb prevented the socially relevant GABAergic long-term potentiation and impaired memory formation after social transmission of food preference. PMID: 28683263
    23. These results support the role of Igf1r in controlling the kinetics of cell proliferation and differentiation during pulmonary airway epithelial regeneration after injury. PMID: 27861515
    24. Although smaller than WT (wild type) mice, old-aged Igf1r(+/-) had increased adiposity and exhibit increased lipogenesis. We hypothesize that IGF-1 resistance initially causes a transient increase in insulin signaling thereby promoting a lipogenic phenotype, which subsequently leads to insulin resistance. PMID: 28351623
    25. HASF is a novel ligand of IGF1R. PMID: 28096202
    26. Developmental deletion of the IGF-IR did not result in any physiological abnormalities or retardation in intestinal growth and development. PMID: 28096258
    27. Under nonpathological conditions, IGF-1 regulates brain IGF-1R. Because baseline IGF-1R levels are rapidly restored, a tight control of brain IGF-1R expression seems to operate under physiological conditions. PMID: 27792405
    28. MicroRNA-99a inhibits insulin-induced proliferation, migration, dedifferentiation, and rapamycin resistance of vascular smooth muscle cells by inhibiting IGF-1R and mTOR signaling. PMID: 28315335
    29. These results indicated that IGF1R may increase cell viability under hypoxic conditions by promoting autophagy and scavenging reactive oxygen species production, which is closed with PI3K/Akt/mTOR signaling pathway. PMID: 28260056
    30. Suggest that overexpression of IGF-IR or IRA isoform, as homodimers or as part of IRA/IGF-IR hybrid receptors, confers a stronger migratory capability to vascular smooth muscle cells and might occur in early stages of atherosclerotic process. PMID: 27905925
    31. Macrophage IGF1R signaling suppresses macrophage and foam cell accumulation in lesions and reduces plaque vulnerability, providing a novel mechanism whereby IGF-1 exerts antiatherogenic effects in ApoE knockout mice. PMID: 27154724
    32. study recognized the promotion of miR-223 level by AGE-BSA treatment in osteoblast-like MC3T3-E1 cells. The promoted miR-223 targeted IGF-1R and mediated the AGE-BSA-induced apoptosis in MC3T3-E1 cells. PMID: 26893485
    33. osterix is a downstream target of IGF1R in chondrocytes. PMID: 26475121
    34. this study, we have characterized epigenetic changes following pregnancy and found that Igf1r and other IGF family members are hypermethylated and downregulated. PMID: 26290394
    35. IGF1R signaling was necessary for DC-mediated T-ALL survival. PMID: 26862168
    36. mitochondria, activated by IGF-1R signaling, constitute a critical regulator of information processing in hippocampal neurons. PMID: 26804996
    37. IGF1R is involved in osteoblast differentiation during fracture repair and it plays an important role in coordinating chondrocyte, osteoclast, and endothelial responses that contribute to endochondral bone formation required for normal fracture repair PMID: 25801198
    38. ectopic down-regulation of IGF1R reversed the protective effect of miR-100 down-regulation on H2O2-induced apoptosis, revealing that miR-100 regulates cardiomyocyte apoptosis through the association of IGF1R. PMID: 26191255
    39. Data (including data from studies in knockout mice) suggest that Igf1r plays role in cardiomyocytes during ventricular remodeling of aging; deletion of Igf1r in cardiomyocytes attenuates cardiac aging in male mice. PMID: 26469138
    40. The IGF1R ectodomain maintains an autoinhibited state in which the transmembrane domains are held apart. PMID: 25255214
    41. Data (including data from studies in knockout mice) suggest that, in aging and metabolic stress such as insulin resistance/pregnancy, pancreatic beta-cells exhibit sex differences in Igf2/Igf1r signaling during beta-cell expansion/mass adaptation. PMID: 26384384
    42. IGF1 signaling plays a greater role in the skeletal actions of cPTH in the female mouse than in the male mouse, which may underlie the sex differences in the response to cPTH. PMID: 25502173
    43. Lung fibroblasts of TSHR KO mice have decreased cell surface Igf1r expression, and have Igf1r protein and protein fragments in both cytoplasm and nucleus. Igf1r mRNA levels were similar between TSHR KO and WT mice. PMID: 26389690
    44. provide evidence of a new role of Transthyretin as a transcription inducer of insulin-like growth factor receptor I in central nervous system, unveiling a new role in neuroprotection PMID: 25084758
    45. IGF-IR regulates the cell fate determination of BCR/ABL(+) leukemia cells and supports the self-renewal of chronic myeloid leukemia cells. PMID: 25648584
    46. Decreased expression of IGF-1R impairs endothelial function and increases the fibrosis of kidney disease. PMID: 25783760
    47. neither IR nor IGF1R deletion prevented a reduction in radiation-induced CEC apoptosis during obesity and hyperinsulinemia PMID: 26251471
    48. Tumor multiplicity was significantly increased while tumor latency was significantly decreased when the IGF-IR transgene was expressed during pubertal development compared to post-pubertal transgene expression. PMID: 25259518
    49. UBC-CreERT2 transgenic mice efficiently delete Igf1r upon postnatal tamoxifen treatment in multiple mouse organs, and corroborate that IGF1R function is highly dependent on cell, tissue and organ type PMID: 25238791
    50. Reducing Igf-1r levels led to some beneficial effects in Huntington disease females, but also led to some detrimental effects in HD males. PMID: 25140802

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 数据库链接：

    KEGG: mmu:16001

    STRING: 10090.ENSMUSP00000005671

    UniGene: Mm.275742