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  4. Recombinant Mouse Interferon regulatory factor 3 (Irf3)

Recombinant Mouse Interferon regulatory factor 3 (Irf3)

In Stock
  • 中文名称：
    小鼠Irf3重组蛋白
  • 货号：
    CSB-YP011818MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Irf3; Interferon regulatory factor 3; IRF-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    Yeast
  • 分子量：
    50.9kDa
  • 表达区域：
    1-419aa
  • 氨基酸序列
    METPKPRILPWLVSQLDLGQLEGVAWLDESRTRFRIPWKHGLRQDAQMADFGIFQAWAEASGAYTPGKDKPDVSTWKRNFRSALNRKEVLRLAADNSKDPYDPHKVYEFVTPGARDFVHLGASPDTNGKSSLPHSQENLPKLFDGLILGPLKDEGSSDLAIVSDPSQQLPSPNVNNFLNPAPQENPLKQLLAEEQWEFEVTAFYRGRQVFQQTLFCPGGLRLVGSTADMTLPWQPVTLPDPEGFLTDKLVKEYVGQVLKGLGNGLALWQAGQCLWAQRLGHSHAFWALGEELLPDSGRGPDGEVHKDKDGAVFDLRPFVADLIAFMEGSGHSPRYTLWFCMGEMWPQDQPWVKRLVMVKVVPTCLKELLEMAREGGASSLKTVDLHISNSQPISLTSDQYKAYLQDLVEDMDFQATGNI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO transfected the expression vector which inserted the recombinant DNA into the yeast, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 10xHis tag and C-terminal Myc tag sequence was appended to the gene coding for the yeast of the mouse Irf3 protein to form the recombinant DNA. The recombinant mouse Irf3 was expressed as N-terminal 10xHis-tagged and C-terminal Myc-tagged fusion. The purity of the protein is greater than 90% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 50 kDa.

    IFN regulatory factor-3 (IRF-3) is a transcription factor that plays a central role in the host IRF-3 regulates genes in the innate immune response. As a key regulator, IRF-3 influence the IFN-α/β gene expression. IRF-3 activation and, as a consequence, IFN-β mRNA induction are inhibited in wild-type (PR8) influenza virus-infected cells but not in cells infected with an isogenic virus lacking the NS1 gene. Studies found that positiveregulationofinterferon regulatory factor 3activation by Herc5 via ISG15 modification. In addition, ubiquitously expressed IRF-3 is directly activated after virus infection and functions as a key activator of the immediate-early alpha/beta interferon (IFN) genes, as well as the RANTES chemokine gene.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
  • 基因功能参考文献：
    1. this study shows that activation of IRF3 contributes to IFN-gamma and ISG54 expression during the immune responses to B16F10 tumor growth PMID: 28651122
    2. IRF3 exacerbates P. aeruginosa-induced mortality in mice by inhibiting neutrophil adhesion and recruitment to the lungs PMID: 28181039
    3. IRF3 expression and activation depend on the signal transduction of the the urotensin II/urotensin receptor (UII/UT) system, and play important roles in UII/UT PMID: 27448985
    4. Interruption of IRF3-dependent signaling resulted in decreased cardiac expression of inflammatory cytokines and chemokines and decreased inflammatory cell infiltration of the heart, as well as in attenuated ventricular dilation and improved cardiac function. PMID: 29106401
    5. This study reveals a critical role of NSD3-mediated IRF3 methylation in enhancing antiviral innate immunity. PMID: 29101251
    6. Data show that stimulator of interferon genes (STING)-associated vasculopathy with onset in infancy (SAVI)-associated STING N153S mutation triggers IRF3-independent immune cell dysregulation and lung disease in mice. PMID: 28951494
    7. study provided evidence that the ability of ICP34.5 to control IRF3 activation is through its ability to reverse translational shutoff and sustain the expression of other IFN inhibitors encoded by the virus PMID: 28904192
    8. c-Cbl negatively regulates IFN-beta signaling and cellular antiviral response by promoting IRF3 ubiquitination and degradation. PMID: 27503123
    9. This study did not find non-conservative mutations among SJL, B10.S, B6 and B10 mice in the IRF3 amino acid sequence, and show SJL bone marrow-derived macrophages infected with Theiler's murine encephalomyelitis virus exhibit increased virus RNA replication and infectious virus yields as well as greater IL-6 production than C57Bl strain (including B10.S) cultures. PMID: 28898711
    10. NEMO-IKKbeta Are Essential for IRF3 and NF-kappaB Activation in the cGAS-STING Pathway PMID: 28939760
    11. The IRF-3 pathway is essential for tick-borne encephalitis virus-induced RANTES production in the brain. PMID: 27576490
    12. Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling. PMID: 27742471
    13. IRF3 is a major transcriptional regulator of adipose inflammation and is involved in maintaining systemic glucose and energy homeostasis PMID: 27400129
    14. Findings indicate that STING-TBK1-IRF3 pathway mediates a crosstalk between ER stress and apoptosis during M. bovis infection, which can effectively control intracellular bacteria. PMID: 28018864
    15. GPR146 has an antiviral role in fighting against viral infection, although the GPR146-mediated protection is eliminated by IRF3/HES1-signalling. PMID: 28464285
    16. this study shows that IRF-3-mediated apoptosis of virus-infected cells could be an effective antiviral mechanism, without expression of the interferon-stimulated genes PMID: 27178468
    17. Results establish Irf3, known mostly for its role in antiviral responses, as a transcription factor involved in the induction of Th2 responses through the promotion of pro-Th2 costimulation in CD11b(+) dendritic cells PMID: 27546168
    18. Rubicon specifically interacts with the interferon regulatory factor (IRF) association domain (IAD) of IRF3, and this interaction leads to inhibition of the dimerization of IRF3, which negatively regulates interferon-mediated antiviral response. PMID: 28468885
    19. this study shows that IRF3 in antigen-presenting cells and T cells is required for optimal T-cell effector function and the ability of T cells to influence innate immune function of antigen-presenting cells PMID: 27641636
    20. The mitochondrial damage-cGAS-STING-IRF3 pathway is critically involved in metabolic stress-induced endothelial inflammation. PMID: 28302626
    21. TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon-beta have been identified. PMID: 28159912
    22. this study shows that lipopolysaccharide priming of dendritic cells stabilizes their tolerogenicity through reduction of NF-kappaB and IRF3 signaling PMID: 27859049
    23. this study shows that regulation of the IRF3 pathway can affect multiple cell types and contribute to ameliorate pathogenesis of infection-triggered exacerbation of chronic obstructive pulmonary disease PMID: 28363903
    24. Data show that the formation of a tripartite ribosomal protein S6 kinase 1 (S6K1)-STING membrane protein-TANK-binding kinase 1 (TBK1) complex was necessary for the activation of interferon regulatory factor-3 (IRF3). PMID: 27043414
    25. characterizes SREBP cleavage-activating protein as an essential adaptor in the STING signaling pathway PMID: 26900919
    26. Study identifies crosstalk between PTEN and IRF3 in tumor suppression and innate immunity. PMID: 26692175
    27. results revealed a new paradigm in which the antiviral host factor, IRF3, plays a cell-intrinsic pro-parasitic role. PMID: 25811886
    28. Irf3/IFN activation in hematopoietic stem and progenitor cells expands multipotent progenitors fractions but inhibits HSC mobilization PMID: 25843711
    29. find that IRF3 versus ISGF3 specificity may be critical to limiting IFN-beta production and ISGF3 activation, temporally and spatially, but that partial overlap in their specificity is tolerable and may enhance the effectiveness of the innate-immune response PMID: 25896227
    30. Bipartite nuclear localization signal controls nuclear import and DNA-binding activity of IRF3. PMID: 25994966
    31. PP1 directly interacts with IRF3 and dephosphorylates IRF3 at Ser385 and Ser396, resulting in the suppression of TLR- and RLR-triggered IFN-beta production. PMID: 25239187
    32. our results identify CK2 as a novel regulator of TBK1 and IRF3 and suggest that targeting CK2 by small molecular inhibitor may be a viable approach to prevent and treat viral infections. PMID: 25810395
    33. DNA-PK is a DNA sensor for IRF-3-dependent innate immunity. PMID: 23251783
    34. These data show that IRF3 plays a crucial role in development of Th17 responses and EAE and warrants investigation in human multiple sclerosis. PMID: 25069698
    35. identified IRF3 as a novel regulator of peroxisome proliferator-activated receptor gamma PMID: 24596398
    36. findings demonstrate that IFN-beta, but not IFN-lambda2/3, produced via an IPS-1-IRF3 signaling pathway, is important for hMPV clearance. PMID: 24726644
    37. both IRF3 and IRF4 are induced by adiponectin, with adiponectin-AMPK and adiponectin-PPARalpha signalling regulating IRF3 and IRF4, respectively. PMID: 24627105
    38. These results implicate mitochondria and peroxisomes as new sites for IRF3 regulation by N(pro), and highlight the role of these organelles in the anti-viral pathway. PMID: 24551175
    39. Altogether, IRF3 contributes to early control of Theiler's murine encephalomyelitis virus replication through induction of IL-6 and IFN-beta and support of IL-6 antiviral effects, but contributes to virus-induced hippocampal injury. PMID: 24140628
    40. IRF3 interacts with IKKbeta in the cytoplasm to inhibit IKKbeta/NF-kappaB signaling, thus alleviating hepatic inflammation, insulin resistance, and hepatic steatosis. PMID: 24123166
    41. IRF3 is a key factor controlling melanoma growth through NK-cell activities PMID: 24368188
    42. We describe increased MAIDS resistance in infected IRF-3 knockout and double knockout mice. PMID: 24335302
    43. Optineurin is dispensable for NF-kappaB activation but necessary for optimal IRF3 activation in immune cells. PMID: 24244017
    44. STING-IRF3 pathway links endoplasmic reticulum stress with hepatocyte apoptosis in early alcoholic liver disease. PMID: 24052526
    45. Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. PMID: 23918362
    46. In summary, the vaccinia virus N2 protein is an intracellular virulence factor that inhibits IRF3 activity in the nucleus. PMID: 23761407
    47. IRF3 may play a key role in innate immune responses against invasion of prion pathogens. PMID: 23093799
    48. IRF-3's transcriptional activity required its coactivators, beta-catenin and CBP, to be modified by HDAC6-mediated deacetylation and protein kinase C isozyme beta (PKC-beta)-mediated phosphorylation, respectively. PMID: 23532979
    49. IRF3 plays a protective role in Aortic Banding-induced hypertrophic response by inactivating ERK1/2 in the heart. PMID: 23307144
    50. Western equine encephalitis virus activated IRF-3-mediated neuronal innate immune pathways in a replication-dependent manner, and abrogation of IRF-3 function enhanced virus-mediated injury. PMID: 23192868

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion.
  • 蛋白家族：
    IRF family
  • 数据库链接：

    KEGG: mmu:54131

    STRING: 10090.ENSMUSP00000003284

    UniGene: Mm.3960