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  4. Recombinant Mouse Ketohexokinase (Khk)

Recombinant Mouse Ketohexokinase (Khk)

  • 货号：
    CSB-YP012157MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012157MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012157MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Khk
  • Uniprot No.：
  • 别名：
    Khk; Ketohexokinase; EC 2.7.1.3; Hepatic fructokinase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-298
  • 氨基酸序列
    MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL SLLGARCAFM GSLAPGHVAD FLVADFRQRG VDVSQVTWQS QGDTPCSCCI VNNSNGSRTI ILYDTNLPDV SAKDFEKVDL TRFKWIHIEG RNASEQVKML QRIEEHNAKQ PLPQKVRVSV EIEKPREELF QLFSYGEVVF VSKDVAKHLG FQPAVEALRG LYSRVKKGAT LVCAWAEEGA DALGPDGQLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
  • 基因功能参考文献：
    1. This studies provided the first evidence that fructokinase modulates vasopressin synthesis in the SON and secretion from the posterior pituitary into circulation. PMID: 27852737
    2. Fructokinase knockout mice were protected from renal injury both at baseline and after high salt intake (3 wk) compared with wild-type mice. This was associated with higher levels of active (phosphorylated serine 1177) endothelial nitric oxide synthase in their kidneys. PMID: 27465991
    3. Feedforward upregulation of fructolytic and gluconeogenic enzymes specifically requires GLUT5 and KHK and may proactively enhance the intestine's ability to process anticipated increases in dietary fructose concentrations. PMID: 26084694
    4. Significant levels of blood fructose are maintained independent of dietary fructose, KHK, and GLUT5, probably by endogenous synthesis of fructose. PMID: 26316589
    5. myocardial hypoxia actuates fructose metabolism in human and mouse models of pathological cardiac hypertrophy through hypoxia-inducible factor 1alpha (HIF1alpha) activation of SF3B1 and SF3B1-mediated splice switching of KHK-A to KHK-C PMID: 26083752
    6. Fructokinase-knockout mice with delayed hydration were protected from renal injury. Thus, recurrent dehydration can induce renal injury via a fructokinase-dependent mechanism, likely from the generation of endogenous fructose via the polyol pathway. PMID: 24336030
    7. This study demonstrates that blocking KHK and redirecting fructose metabolism to alternative pathways is an effective way to prevent visceral obesity and insulin resistance induced by high fructose, a widespread component of Western diets. PMID: 25187370
    8. These studies identify fructokinase as a novel mediator of diabetic nephropathy and document a novel role for endogenous fructose production, or fructoneogenesis, in driving renal disease. PMID: 24876114
    9. High-fat and high-sucrose (western) diet induces steatohepatitis that is dependent on fructokinase. PMID: 23813872
    10. Fructose-induced metabolic syndrome is prevented in mice lacking both fructokinase B and A but is exacerbated in mice lacking fructokinase A. PMID: 22371574
    11. These studies are the first demonstration that neither Khk isoform is required for normal growth and development. PMID: 20841500

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  • 蛋白家族：
    Carbohydrate kinase PfkB family
  • 数据库链接：

    KEGG: mmu:16548

    STRING: 10090.ENSMUSP00000031053

    UniGene: Mm.22451