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  4. Recombinant Mouse Kinesin-like protein KIF3A (Kif3a)

Recombinant Mouse Kinesin-like protein KIF3A (Kif3a)

  • 中文名称：
    小鼠Kif3a重组蛋白
  • 货号：
    CSB-YP012335MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Kif3a重组蛋白
  • 货号：
    CSB-EP012335MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Kif3a重组蛋白
  • 货号：
    CSB-EP012335MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Kif3a重组蛋白
  • 货号：
    CSB-BP012335MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Kif3a重组蛋白
  • 货号：
    CSB-MP012335MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Kif3a; Kif3Kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-701
  • 氨基酸序列
    MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYRQAVSVD EMRGTITVHK TDSSNEPPKT FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG QTGTGKTFTM EGVRAVPGLR GVIPNSFAHI FGHIAKAEGD TRFLVRVSYL EIYNEEVRDL LGKDQTQRLE VKERPDVGVY IKDLSAYVVN NADDMDRIMT LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGVDGNMH VRMGKLHLVD LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR INEDPKDALL RQFQKEIEEL KKKLEEGEEV SGSDISGSEE DDEEGELGED GEKKKKRRDQ AGKKKVSPDK MVEMQAKIDE ERKALETKLD MEEEERNKAR AELERREKDL LKAQQEHQSL LEKLSALEKK VIVGGVDLLA KAEEQEKLLE ESNMELEERR RRAEQLRKEL EEKEQERLDI EEKYTSLQEE AQGKTKKLKK VWTMLMAAKS EMADLQQEHQ REIEGLLENI RQLSRELRLQ MLIIDNFIPQ DYQEMIENYV HWNEDIGEWQ LKCVAYTGNN MRKQTPVPDK KERDPFEVDL SHVYLAYTEE SLRQSLMKLE RPRTSKGKAR PKMGRRKRSA KPETVIDSLL Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
  • 基因功能参考文献：
    1. KIF3A plays an important role in epithelium apoptosis and bronchial inflammation in asthma PMID: 29156524
    2. Cell senescence is a central feature in nephronophthisis type 7 and Kif3a is unexpectedly required for efficient DNA damage response and cell cycle arrest. PMID: 27181777
    3. The results show for KIF3AB that the kinetics of microtubule association is less than the rates observed for KIF3AA or KIF3BB. PMID: 27637334
    4. genetically ablated Kif3a, Ift88, and Ttc21b in a series of specific spatiotemporal domains. The resulting phenotypes allow us to draw several conclusions. First, we conclude that the Ttc21b cortical phenotype is not due to the activity of Ttc21b within the brain itself PMID: 28291836
    5. Airway epithelial KIF3A suppresses Th2 pulmonary inflammation and airway hyperresponsiveness following aeroallergen exposure, implicating epithelial microtubular functions in the pathogenesis of Th2-mediated lung pathology. PMID: 27794000
    6. Longitudinal microcomputed tomography (muCT) imaging and histopathological analyses revealed an increased rate of cyst formation, increased proportion of cysts with proliferating cells, higher frequency of atypical cysts as well as the development of neoplasms in Vhl/Kif3a/Trp53 mutant kidneys compared to Kif3a/Trp53 or Vhl/Kif3a mutant kidneys. PMID: 27126173
    7. Study found that both KIF3AC and KIF3AB can traverse long distances over the microtubule track, revealing that these motors are adept and well-tuned for their specialized roles as cargo transporters PMID: 26445448
    8. Data suggest biocatalysis by Kif3a homodimer, Kif3c homodimer, or Kif3a/Kif3c heterodimer vary; microtubule interaction is very fast for Kif3a homodimer; Kif3c homodimer interaction is much slower; Kif3a/Kif3c heterodimer interaction is intermediate. PMID: 26710851
    9. KIF1-binding protein interacts with KIF3A in haploid male germ cells, promoting spermatid elongation. PMID: 26245936
    10. Vhl and Kif3a deletion accelerates renal cyst formation PMID: 25788526
    11. The results of this study suggested thatactivity-dependent cargo loading, in which phosphorylation of the KIF3A C terminus upregulates the loading and transport of N-cadherin in homeostatic synaptic plasticity. PMID: 26335646
    12. The results demonstrate that visual pigments transport to the retinal outer segment despite removal of KIF3 and IFT88, and KIF3-mediated anterograde IFT is responsible for photoreceptor transition zone and axoneme formation. PMID: 25825494
    13. Processivity of the kinesin-2 KIF3A results from rear head gating and not front head gating. PMID: 25657001
    14. A ciliopathy with hydrocephalus, isolated craniosynostosis, hypertelorism, and clefting caused by deletion of Kif3a PMID: 24887031
    15. KIF3AB exhibits novel ATPase activity. PMID: 25122755
    16. single-molecule motility from different kinesin-1 and kinesin-2 neck-linker chimeras stepping along microtubules, was studied. PMID: 24739168
    17. This study highlights the essential role of KIF3A-mediated microtubular transport in the development of spermatozoa and male fertility. PMID: 23831641
    18. Kif3a controls nephron number via distinct cell lineage-specific mechanisms. PMID: 23762375
    19. Thyroid-specific inactivation of KIF3A alters the TSH signaling pathway and leads to hypothyroidism. PMID: 23511952
    20. findings show that KIF3a is necessary for medulloblastoma formation PMID: 23389290
    21. in addition to its ciliogenic roles, Kif3a recruits p150(Glued) to the subdistal appendages of mother centrioles, critical for centrosomes to function as microtubule-organizing centres. PMID: 23386061
    22. Disruption of Kif3a in osteoblasts results in defective bone formation and osteopenia. PMID: 22357948
    23. The Axin/TNKS2/KIF3A complex is critical for GLUT4 translocation in response to insulin. PMID: 22473005
    24. Deletion of Kif3A reduces bone formation due to loading. PMID: 22428034
    25. our data suggest that RNF33 most likely interacts with KIF3A-KIF3B independent of the adaptor kinesin-associated protein KAP3 PMID: 21909995
    26. show that the microtubule motor subunit Kif3a regulates hair cell polarization through both ciliary and non-ciliary mechanisms PMID: 21752934
    27. Kif3a and primary cilia are required for coordination of chondrocyte maturation, intramembranous bone formation, and chondrogenic condylar growth PMID: 21566205
    28. Pkd1 and Kif3a counterbalance the regulation of osteogenesis and adipogenesis through differential regulation of Runx2 and PPARgamma by Gli2 PMID: 21151991
    29. Epidermal cilia function was analyzed using conditional alleles of the ciliogenic genes Ift88 and Kif3a. PMID: 21429982
    30. KIF3, cilia, and nodal flow have roles in left-right determination [review] PMID: 20066075
    31. The dynamins comprise a large family of mechanoenzymes known to participate in membrane modeling events. All three conventional dynamin genes (Dnm1, Dnm2, Dnm3) are expressed in mammalian brain and produce more than 27 different dynamin proteins. PMID: 12646135
    32. role in inhibiting renal ciliogenesis and produces polycystic kidney disease PMID: 12672950
    33. insulin signaling stimulates Rab4 activity, the association of Rab4 with kinesin, the interaction of KIF3 with microtubules and this process is mediated by insulin-induced PI3-kinase-dependent PKC-lambda activation and participates in GLUT4 exocytosis PMID: 12832475
    34. Interaction of KIF3A/3B with KAP3 may be important for the proper progression of mitosis. PMID: 16298999
    35. Tissue-specific loss of Kif3a in pancreatic cells resulted in severe pancreatic abnormalities including acinar-to-ductal metaplasia, fibrosis, and lipomatosis. PMID: 17123526
    36. Kif3a, a subunit of the Kinesin II motor complex, is required for intraflagellar transport and the formation of cilia. PMID: 17359961
    37. Kif3a and primary cilia make unique contributions to cranial base development and synchondrosis growth plate function. PMID: 17507416
    38. Gli1 transcript level is dramatically reduced, whereas Gli3 expression is not significantly affected by kinesin-2 depletion. However, the motor is required for the efficient cleavage of the full-length Gli3 transcription factor into a repressor form. PMID: 17698054
    39. Results suggest that Kif3a restrains canonical Wnt signalling both by restricting the CKI-dependent phosphorylation of Dvl and through a separate ciliary mechanism. PMID: 18084282
    40. Kidney-specific inactivation of Kif3a in newborn mice resulted in the loss of primary cilia and produced kidney cysts primarily in the loops of Henle. PMID: 18263895
    41. findings indicate that, together, beta-arrestins and Kif3A may mediate the transport of Smo to the primary cilium PMID: 18497258
    42. Disruption of FKBP8 function activates the Shh signaling pathway cell-autonomously dependent on the Gli2 transcription factor and kinesin-2 subunit Kif3a, a component of the intraflagellar transport machinery used to generate cilia. PMID: 18590716
    43. effect of KIF3A deletion on transport of phototransduction proteins was studied in cone and rod photoreceptors; results indicate that trafficking of membrane proteins to the outer segment is dependent on kinesin-II in cone, but not rod photoreceptors PMID: 19906976

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily
  • 组织特异性：
    Expressed almost exclusively in adult brain tissue (mainly in the cerebellar granular layer) within a single type of neuronal cell.
  • 数据库链接：

    KEGG: mmu:16568

    STRING: 10090.ENSMUSP00000112782

    UniGene: Mm.88694