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  4. Recombinant Mouse Mediator of RNA polymerase II transcription subunit 25 (Med25)

Recombinant Mouse Mediator of RNA polymerase II transcription subunit 25 (Med25)

  • 中文名称：
    小鼠Med25重组蛋白
  • 货号：
    CSB-YP837700MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Med25重组蛋白
  • 货号：
    CSB-EP837700MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Med25重组蛋白
  • 货号：
    CSB-EP837700MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Med25重组蛋白
  • 货号：
    CSB-BP837700MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Med25重组蛋白
  • 货号：
    CSB-MP837700MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Med25
  • Uniprot No.：
  • 别名：
    Med25Mediator of RNA polymerase II transcription subunit 25; Mediator complex subunit 25; mMED25
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-745
  • 氨基酸序列
    MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTESLV QKIGERGIHF SIVSPRKLPA LRLLFEKAAP PALLEPLQQP ADVSQDPRHM VLVRGLVLPV GGSSTSGSLQ TKQAVPLPPA PASAATLSAA PPQALPPVPP QYQVPGNLSA AQVAAQNAVE AAKSQKAGLG PRFSPINPLQ QAAPGVGPPF SQAPAPPLAP VPPGAPKPPP ASQPSLVSTV APGPVLAAPA QPGAPSLAGT VTPGGVNGPS AAQLGGPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITNHKQVQQQ KLEQQRGMGA QQAPPVLGPI LEEQARPPQN LLQLRAPQPQ PQGAVGASAA TGQPQPQGAT QAPTGAPQGP PGAAPGPPPS GPILRPQNPG ANPQLRSLLL NPAPPQTGVP PPQASLHHLQ PPGAPTLLPP HQSMGQPQLG PQLLHPPPAQ SWPTQLPQRA PLPGQMLLSG GPRGPVPQPG LQPSVMEDDI LMDLI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.
  • 基因功能参考文献：
    1. the identification of MED25 as one of the HNF4alpha binding partners in pancreatic ss-cells leading to insulin secretion which is impaired in MODY patients PMID: 22952853
    2. Data show that Med25 is coordinately expressed with Pmp22 gene dosage and expression in transgenic mice and rats, and suggest a potential role of this protein in the molecular etiology of Charcot-Marie-Tooth disease. PMID: 19290556
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Mediator complex subunit 25 family
  • 数据库链接：

    KEGG: mmu:75613

    STRING: 10090.ENSMUSP00000003049

    UniGene: Mm.157778