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  4. Recombinant Mouse Meiotic recombination protein REC8 homolog (Rec8)

Recombinant Mouse Meiotic recombination protein REC8 homolog (Rec8)

  • 货号：
    CSB-YP806492MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806492MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806492MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806492MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806492MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rec8
  • Uniprot No.：
  • 别名：
    Rec8; Mei8; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-591
  • 氨基酸序列
    MFYYPNVLQR HTGCFATIWL AATRGSRLVK REYLNVNVVK TCEEILNYVL VRVQPPVAGL PRPRFSLYLS AQLQIGVIRV YFQQCQYLVE DIQHILEHLH RAQLRIRIDM EEADLPSLLL PNCLAMMETL EDAPEPFFGK MSVDPRLPSP FDIPQIRHLL EAATPEKTRK ETLPEATPDP RKPDRTLATV QSPEVITLQE AEPIRMLQIE GEQDLPEISR GDLELLIAEK DDAILLEERQ RGRLLRQRRA SLPLDESREE PRALEGAGLV SALSPPAPAQ VEGIQEALPG QVFPPEVQKM TGWEPGALLT EVTPPQELRL PAPPSTEKRL PSLQRPLPRR HRRRQLLFWD KETQISREKF EEQLQTGAHC WEYPVAQPPK RMLTSPAELF RTPTLSGWLP PELLGLWTHC AQVPQRMLRQ RPQLETEETV EEERAADEEE RRKTEALSEI EVLREAQEPS GPLMLSSELS LEAAEDEKSR TSLIPPEWWA WSEEGQPEPP ALPMLPELPE VPMEMPPRPE LSSEAVLRAV ALKLQANKEL DFSSLVPPLS PRKLASRVFY LLLVLSTQKI LLVEQQKPYG PLLIRPGPKF P
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
  • 基因功能参考文献：
    1. REC8-containing cohesin complexes provide canonical SCC, not RAD21 or RAD21L, at the prophase stage of meiosis I in spermatocytes. PMID: 27170622
    2. Rec8 establishes cohesion when activated during DNA replication in fetal oocytes using tamoxifen-inducible Cre. In contrast, no new cohesion is detected when Rec8 is activated in arrested oocytes by tamoxifen despite cohesin synthesis. PMID: 26898469
    3. Plk1 activity is required for meiotic spindle assembly and REC8 cleavage, with pPlk1Ser137 being the action executor, in mouse oocytes during meiotic division. PMID: 26654596
    4. effects ofAte1ablation are inferred to be caused, at least in part, by the failure to destroy the C-terminal fragment of Rec8 in the absence of N-terminal arginylation. PMID: 26858254
    5. Findings indicate that cohesin-mediated long-range interactions facilitate discrete gene expression states within preexisting chromosomal compartments. PMID: 24002784
    6. Data show that nociceptin induces Rec8 phosphorylation, triggering chromosome dynamics, in spermatocytes during meiosis in postnatal testes. PMID: 23720425
    7. We report here evidence from the mouse that partial loss of gene function for either Smc1b or Rec8 causes perturbations in the formation of the synaptonemal complex (SC) and affects both synapsis and recombination between homologs during meiotic prophase PMID: 23408896
    8. show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair PMID: 22711701
    9. Separase is regulated by two independent mechanisms in meiosis I, binding to securin and inhibitory phosphorylation by CDK1; both must be disrupted to have any effect on either chromosome-associated REC8 levels or premature chromosome separation. PMID: 21865557
    10. Rec8 cleavage triggers chiasmata resolution during meiosis I and sister centromere disjunction during meiosis II PMID: 20971813
    11. Analyses of mRNA expression patterns of cohesin subunits Rad21 and Rec8 in mice: germ cell-specific expression of rec8 mRNA in both male and female mice. PMID: 12130806
    12. Rec8 protein, in association with SMC3 and SMC1beta but not SMC1alpha, is involved in meiosis-specific chromosome behavior; homologous chromosome separation is triggered by selective loss of Rec8 from chromosome arms in meiosis I. PMID: 12759374
    13. a major role for REC8 in mammalian meiosis is to limit synapsis to between homologous chromosomes. PMID: 15935783
    14. Proteolytic activity of separase is therefore essential for Rec8's removal from chromosome arms and for chiasma resolution but not for PBE. PMID: 16839882
    15. loss of armRec8 (homolog separation) and cytokinesis are suppressed until anaphase I by Securin PMID: 16855401

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  • 亚细胞定位：
    Nucleus. Chromosome. Chromosome, centromere.
  • 蛋白家族：
    Rad21 family
  • 组织特异性：
    Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues inc
  • 数据库链接：

    KEGG: mmu:56739

    STRING: 10090.ENSMUSP00000002395

    UniGene: Mm.23149