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  4. Recombinant Mouse Melanophilin (Mlph)

Recombinant Mouse Melanophilin (Mlph)

  • 货号：
    CSB-YP014640MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014640MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014640MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014640MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014640MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mlph
  • Uniprot No.：
  • 别名：
    Mlph; Ln; Slac2aMelanophilin; Exophilin-3; Leaden protein; Slp homolog lacking C2 domains a; SlaC2-a; Synaptotagmin-like protein 2a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-590
  • 氨基酸序列
    MGKRLDLSTL TDEEAEHVWA VVQRDFDLRR REEERLQGLK GKIQKESSKR ELLSDTAHLN ETHCARCLQP YRLLLNSRRQ CLECSLFVCK SCSHAHPEEQ GWLCDPCHLA RVVKIGSLEW YYQHVRARFK RFGSAKVIRS LCGRLQGGGG SEPSLEEGNG DSEQTDEDGD LDTEARDQPL NSKKKKRLLS FRDVDFEEDS DHLVQPCSQT LGLSSVPESA HSLQSLSGEP YSEDTTSLEP EGLEETGARA LGCRPSPEVQ PCSPLPSGED AHAELDSPAA SCKSAFGTTA MPGTDDVRGK HLPSQYLADV DTSDEDSIQG PRAASQHSKR RARTVPETQI LELNKRMSAV EHLLVHLENT VLPPSAQEPT VETHPSADTE EETLRRRLEE LTSNISGSST SSEDETKPDG TFLGGSPKVC TDTGHMETQE RNPRSPGNPA RPTKSTDEEL SEMEDRVAMT ASEVQQAESE ISDIESRIAA LRAAGLTVKP SGKPRRKSGI PIFLPRVTEK LDRIPKTPPA DPDDQAKMPK ATTAVPSLLR RKYSPSSQGV DSGSFDRKSV YRGSLTQRNP NGRRGTARHI FAKPVMAQQP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • 基因功能参考文献：
    1. When actin and microtubules were present simultaneously, melanophilin's phosphorylation state enforced track selection of the Rab27a/melanophilin/myosin Va transport complex. PMID: 28559319
    2. The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin. PMID: 24584932
    3. Data show at the single-molecule level that melanophilin (Mlph) increases the number of processively moving myoVa motors by 17-fold. PMID: 23979131
    4. Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport PMID: 11856727
    5. The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes PMID: 11886590
    6. myosin Va binds to melanophilin and Rab27a PMID: 11887186
    7. Melanophilin, the product of the leaden locus, is required for targeting of myosin-Va to melanosomes PMID: 11929602
    8. SHD1 of Slac2-a/melanophilin alone is both necessary and sufficient for high affinity specific recognition of the GTP-bound form of Rab27A PMID: 12189142
    9. Slac2-a organizes actin-based melanosome transport in cooperation with Rab27A, myosin Va, and actin PMID: 12861011
    10. cAMP stimulates the expression of Rab27a and rapidly increases the interaction of the melanophilin/Slac2-a with actin PMID: 15059972
    11. protein degradation of Slac2-a is an essential process for proper melanosome distribution in melanocytes PMID: 15145961
    12. Here we found that melanophilin directly activates the actin-activated ATPase activity of myosin Va and thus its motor activity. This is the first demonstration that the binding of the cargo molecule directly activates myosin motor activity. PMID: 15760894
    13. These results highlight the importance of the coiled-coil region together with Rab27a-binding domain (R27BD) and exon F-binding region (EFBD) regions of Mlph in the formation of the active melanosomal Rab27a-Mlph-MyoVa complex. PMID: 16914517
    14. analysis of the minimal myosin Va binding site within an intrinsically unstructured domain of melanophilin PMID: 17513864
    15. The transcript levels of melanophilin, a protein involved in vesicular trafficking in melanocytes, are rapidly increased by aldosterone in cortical collecting duct cells PMID: 17609287
    16. Data suggest that Mlph is targeted to and/or stabilised on melanosomes by Rab27a, and then recruits MyoVa, which provides additional stability to the complex and allows melanosomes to transfer from MT to actin-based transport. PMID: 17698919
    17. The C-terminus of Slac2-a/melanophilin contains a novel actin-binding site, which may be involved in capture of Rab27-containing organelles in the actin-enriched cell periphery. PMID: 12221080
    18. Slac2-a/melanophilin contains an N-terminal Slp homology domain (SHD) (PMID: 11327731). The SHD of Slac2-a specifically and directly binds the GTP-bound form of Rab27A and Rab27B (PMID: 11773082). The C-terminal domain directly binds myosin Va. PMID: 11856727

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  • 亚细胞定位：
    Melanosome.
  • 组织特异性：
    Highly expressed in embryos at day 7; not detectable at day 11. Highly expressed in adult stomach; detected at lower levels in kidney, lung, skin and small intestine. Detected in melanocytes.
  • 数据库链接：

    KEGG: mmu:171531

    STRING: 10090.ENSMUSP00000027528

    UniGene: Mm.105208