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  4. Recombinant Mouse Melanoregulin (Mreg)

Recombinant Mouse Melanoregulin (Mreg)

  • 货号：
    CSB-YP014788MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014788MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014788MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014788MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014788MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mreg
  • Uniprot No.：
  • 别名：
    Mreg; Dsu; Gm974; Wdt2; Melanoregulin; Dilute suppressor protein; Whn-dependent transcript 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-214
  • 氨基酸序列
    MGLRRWLRSA CCCCPCRCLE EPARPEKEPL VSGNNPYSSF GATLERDDEK NLWSMPHDVS HTEADDDRIL YNLIVIRNQQ TKDSEEWQRL NYDIYTLRQI RREVRNRWRR ILEDLGFQRE ADSLLSVTKL STMSDSKNTR KAREMLLKLA EETSIFPASW ELSERYLLVV DRLIALDAAE DFFKIASQMY PKKPGVPCLV DGQRKLHCLP FPSP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably functions as cargo-recognition protein that couples cytoplasmic vesicles to the transport machinery. Plays a role in hair pigmentation, a process that involves shedding of melanosome-containing vesicles from melanocytes, followed by phagocytosis of the melanosome-containing vesicles by keratinocytes. Functions on melanosomes as receptor for RILP and the complex formed by RILP and DCTN1, and thereby contributes to retrograde melanosome transport from the cell periphery to the center. Overexpression causes accumulation of late endosomes and/or lysosomes at the microtubule organising center (MTOC) at the center of the cell. Probably binds cholesterol and requires the presence of cholesterol in membranes to function in microtubule-mediated retrograde organelle transport. Binds phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate and phosphatidylinositol 3,5-bisphosphate, but not phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 4,5-bisphosphate. Required for normal phagosome clearing and normal activation of lysosomal enzymes in lysosomes from retinal pigment epithelium cells. Required for normal degradation of the lipofuscin component N-retinylidene-N-retinylethanolamine (A2E) in the eye. May function in membrane fusion and regulate the biogenesis of disk membranes of photoreceptor rod cells (Probable).
  • 基因功能参考文献：
    1. Single membrane POS containing phagosomes recruit endogenous LC3 in an ATG5-dependent manner that is dependent on MREG. In the Mregdsu/dsu RPE, Atg5/12 and LC3 were increased relative to levels in the Mreg+/+ at all time points relative to light onset. PMID: 25301234
    2. This study demonistrated that Loss of melanoregulin (MREG) enhances cathepsin-D secretion by the retinal pigment epithelium. PMID: 23611523
    3. The product of the Mreg(dsu) locus, melanoregulin (MREG), interacts both with members of BLOC-2 complex and with ocular albinism in regulating melanosome size. PMID: 22984402
    4. Mreg regulates microtubule-dependent retrograde melanosome transport through the dynein-dynactin motor complex. PMID: 22275436
    5. multiple palmitoylation serves to stably anchor melanoregulin in the melanosome membrane. PMID: 22940130
    6. Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes. PMID: 22753477
    7. dsu identifies a unique protein involved in pigmentation of the mammalian hair PMID: 15550542
    8. melanoregulin regulates peripherin-2 function PMID: 17260955
    9. Results suggest that melanoregulin (MREG) is required for lysosome maturation and support a role for MREG in intracellular trafficking. PMID: 19240024

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  • 亚细胞定位：
    Apical cell membrane; Peripheral membrane protein. Melanosome membrane; Lipid-anchor. Lysosome membrane; Lipid-anchor. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Melanoregulin family
  • 组织特异性：
    Detected in melanocytes. Expressed in retina, in retinal pigment epithelium (at protein level). Widely expressed with higher expression in skin, heart, liver, testis and thymus. Detected in retina, in retinal pigment epithelium cells.
  • 数据库链接：

    KEGG: mmu:381269

    STRING: 10090.ENSMUSP00000041878

    UniGene: Mm.422636