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  4. Recombinant Mouse Merlin (Nf2), partial

Recombinant Mouse Merlin (Nf2), partial

In Stock
  • 货号：
    CSB-EP015741MO1
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nf2; Nf-2Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    55.9 kDa
  • 表达区域：
    1-320aa
  • 氨基酸序列
    MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-Trx-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.
  • 基因功能参考文献：
    1. Results demonstrate that loss of merlin function in Schwann Cells (SCs) leads to increased endoneurial space and ultrastructural myelin alterations in the sciatic nerve before and after injury. Loss of merlin function in SCs delays neural recovery following injury. Nevertheless, there were no significant functional or electrophysiological differences in neural regeneration attributable to Nf2 mutation in SCs. PMID: 29408605
    2. Data indicate an essential role for NF2 and the Hippo pathway in regulation of branching morphogenesis in the mammalian kidney. PMID: 27480037
    3. Loss of Nf2 causes hyperplasia and ocular abnormalities. PMID: 29249622
    4. studies suggest that NF2 normally limits biliary morphogenesis by coordinating lumen expansion and cell architecture. This work provides fundamental insight into how biliary fate and tubulogenesis are coordinated during development and will guide analyses of disease-associated and experimentally induced biliary pathologies. PMID: 29712669
    5. Data show that early Smarcb1 loss causes rhabdoid tumors whereas loss at later stages combined with Nf2 gene inactivation causes shwannomas. PMID: 28824165
    6. Rho attenuates the interaction between Amot and Nf2 by binding to the coiled-coil domain of Amot. PMID: 28947533
    7. co-deletion of Rac1 with Nf2 blocks tumor initiation but paradoxically exacerbates hepatomegaly induced by Nf2 loss, which can be suppressed either by treatment with pro-oxidants or by co-deletion of Yap. PMID: 27818180
    8. Merlin controls the repair capacity of Schwann cells after injury by regulating Hippo/YAP signaling activity. PMID: 28137778
    9. NF2 is activated by oxidative stress in cardiomyocytes and myocardium and facilitates apoptosis. PMID: 27402866
    10. loss of axonal contact following nerve injury results in merlin phosphorylation leading to increased p75(NTR) expression. PMID: 26057084
    11. Loss of Nf2 and Cdkn2a/b have synergistic effects with PDGF-B overexpression promoting meningioma malignant transformation. PMID: 26418719
    12. Merlin 1 and 2 act as tumor suppressors and are required for optimal sperm maturation PMID: 26258444
    13. Together our results uncover miRNAs as yet another negative mechanism controlling Merlin tumor suppressor functions. PMID: 26549232
    14. Merlin and Ezrin are components of a mechanism where mechanical forces associated with cell junctions are transduced across the cell cortex via cortical actomyosin cytoskeleton to control lateral mobility and activity of epidermal growth factor receptor. PMID: 26483553
    15. The study describe a novel NF2 mouse model recapitulating schwannoma phenotypes found in human patients where tumors develop in the cranial nerve VIII and/or the spinal roots. PMID: 25113746
    16. Nf2/Merlin controls spinal cord neural progenitor function in a Rac1/ErbB2-dependent manner. PMID: 24817309
    17. Nf2-Yap signaling plays important roles in controlling the expansion of dorsal root ganglia progenitors and glia during DRG development PMID: 25433207
    18. CD44 cytoplasmic tail cleaved by RIP could release DCAF1 from merlin by competing for binding to the merlin FERM domain, which results in the inhibition of merlin-mediated suppression of tumorigenesis. PMID: 24912773
    19. Findings indicate that merlin is sumoylated and that this post-translational modification is essential for tumor suppression. PMID: 24166499
    20. Cdc42 regulates SC radial sorting in vivo through Neurofibromin 2/merlin dependent signaling pathways PMID: 24014231
    21. The data establish a clear role for Nf2 upstream of Yap in the preimplantation embryo and demonstrate that Hippo signaling is essential to segregate the inner cell mass from the trophectoderm. PMID: 23791728
    22. These results indicate that in both Drosophila and mammals, Merlin activates Wts/Lats phosphorylation without stimulating the intrinsic kinase activity of Hpo/Mst. PMID: 24012335
    23. Meningioma progression in mice triggered by Nf2 and Cdkn2ab inactivation. PMID: 23045274
    24. Signaling between Nf2 and Rac1 occurs in a bidirectional fashion, and these interactions are modulated by PKA. PMID: 23045281
    25. Nf2 is required for hippocampal morphogenesis. Yap/Taz are key downstream effectors of Nf2 during brain development. PMID: 23863479
    26. Neurofibromin 2 is an AKAP (A-kinase-anchoring protein) scaffold protein that facilitates the function of the cAMP/PKA-LATS-YAP pathway. PMID: 23644383
    27. propose that Merlin mediates contact inhibition and suppresses tumorigenesis by translocating to the nucleus to inhibit CRL4(DCAF1). PMID: 21878678
    28. Results demonstrate that Schwannomin plays an essential role in inducing and/or maintaining the SC's spindle shape, and by stabilizing the bipolar morphology, Sch promotes the alignment of SCs with axons and ultimately influences myelin segment length. PMID: 21182951
    29. FERM domain-mediated phosphoinositide binding and membrane association are critical for the growth-regulatory function of merlin PMID: 21402777
    30. Depletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo PMID: 21481793
    31. Neurofibromatosis type 2 protein is inactivated in glioblastoma cells by overexpression of ezrin. PMID: 20156804
    32. Results reveal that Merlin can associate directly with alpha-catenin and link it to Par3, thereby providing an essential link between the AJ and the Par3 polarity complex during junctional maturation. PMID: 21074722
    33. a critical role for Nf2/Merlin in controlling homeostasis of the liver stem cell niche PMID: 20675406
    34. Nf2-deficient phenotypes in multiple tissues were largely suppressed by heterozygous deletion of Yap, suggesting that YAP is a major effector of Merlin/NF2 in growth regulation. PMID: 20643348
    35. This study provided merlin plays a pivotal role in controlling the neuronal wiring in the developing CNS. PMID: 20668201
    36. Tumor suppression by merlin is independent of its role as an organizer of the actin cytoskeleton in Schwann cells. PMID: 19910496
    37. Rac1-mediated canonical Wnt signaling is essential for the loss of contact inhibition in NF2-deficient cells. PMID: 20154721
    38. Structural basis for neurofibromatosis type 2 PMID: 11756419
    39. phosphorylation of merlin at serine 518 leads to dramatic protein relocalization PMID: 11782491
    40. Nf2 and p53 mutations do not synergize in meningeal tumorigenesis.Nf2 gene inactivation in arachnoidal cells is rate-limiting for meningioma development in the mouse. PMID: 12000789
    41. Mutant products of the NF2 tumor suppressor gene are degraded by the ubiquitin-proteasome pathway PMID: 12130630
    42. A FERM domain mutant of the Nf2 tumor suppressor gene causes cellular transformation and tumorigenesis in mice PMID: 12203111
    43. Merlin inhibits Pak1;loss of Merlin leads to inappropriate activation of Pak1. Conversely, the overexpression of Merlin leads to inhibition of Pak1 activation. PMID: 14580336
    44. biallelic loss of Nf2 function in neural crest-derived cells hemizygous for p53 results in resistance to osteogenic tumours and increased susceptibility to peripheral nerve sheath tumours PMID: 15221010
    45. erbin regulates MAP kinase activation in Schwann cells and suggest that erbin links merlin to both adherens junction protein complexes and the MAP kinase signaling pathway. PMID: 15659388
    46. NF2 tumor suppressor Merlin and the ERM proteins interact with N-WASP and regulate its actin polymerization function PMID: 15699051
    47. In brain tissue and neuronal progenitor cell cultures merlin was predominantly found in neurons. Merlin expression was seen from Day 11 in mouse embryos. PMID: 15797715
    48. These results suggest that merlin contributes, via its protein-to-protein interaction with Grb2 and consequent inhibition of the MAPK pathways. PMID: 16405865
    49. PP1-delta and MYPT-1 were co-precipitated with endogenous merlin from NIH-3T3 cells PMID: 16885985
    50. inhibition of the CD44-HA interaction contributes to the tumor-suppressor function of merlin, and they suggest that merlin inhibits tumor growth, at least in part, by negatively regulating CD44 function PMID: 16953231

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cytoplasm, cytoskeleton. Nucleus.
  • 数据库链接：

    KEGG: mmu:18016

    STRING: 10090.ENSMUSP00000105536

    UniGene: Mm.297109