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  4. Recombinant Mouse Monoglyceride lipase (Mgll)

Recombinant Mouse Monoglyceride lipase (Mgll)

  • 中文名称：
    小鼠Mgll重组蛋白
  • 货号：
    CSB-EP013787MOa2
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013787MOa2 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mgll.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP013787MOa2 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mgll.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Mgll
  • Uniprot No.：
  • 别名：
    MgllMonoglyceride lipase; MGL; EC 3.1.1.23; Monoacylglycerol lipase; MAGL
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    49.4kDa
  • 表达区域：
    1-303aa
  • 氨基酸序列
    MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAAGAGCPP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
  • 基因功能参考文献：
    1. Neuronal and astrocytic monoacylglycerol lipase contributes to 2-arachidonoylglycerol clearance and prevent CB1 receptor over-stimulation in the cerebellum. PMID: 27775008
    2. Results provide evidence that MGL deficiency causes complex changes in cholesterol metabolism and in the regulation of gut transit. PMID: 28380440
    3. N-arachidonoyl ethanolamine and 2-arachidonoyl glycerol hydrolyzing enzymes, FAAH and MAGL, and the CB1 receptor link the endocannabinoid system to broader lipid signaling networks in contrasting ways, potentially altering neurotransmission and behavior independently of cannabinoid receptor signaling. PMID: 27109320
    4. Results suggest that neuronal and astrocytic MAGL collaborate to terminate endocannabinoid-mediated synaptic suppression and prompt synapse-specificity of endocannabinoid signaling in the cerebellum. PMID: 27182552
    5. Activities of adipose triglyceride lipase (ATGL), hormone sensitive lipolitic enzyme (HSL) and monoacylglycerol lipase (MGL) were significantly higher (51 %, 38 %, 49 %) in the DE group than the HF group (p < 0.05). MGL, there were no differences between the CO group, HF group, and DC group, with the DE group (70 %) being significantly higher (p < 0.05). PMID: 27596982
    6. MGL in astrocytes is an important regulator of 2-arachidonoylglyerol levels, arachidonic acid availability, and neuroinflammation. PMID: 26565024
    7. Genetic and pharmacological ablation of Magl attenuated centrally-mediated fever response. PMID: 26287872
    8. the results indicate that global MGL deletion leads to systemic changes that produce a leaner phenotype and an improved serum metabolic profile. PMID: 25842377
    9. This study showed that Genetic deletion of monoacylglycerol lipase leads to impaired cannabinoid receptor CBR signaling and anxiety-like behavior. PMID: 26223500
    10. Suggest that organophosphate agents induce plasma hypertriglyceridemia in mouse through single or dual inhibition of FAAH or/and MAGL, apparently leading to overstimulation of cannabinoid signal regulating energy metabolism. PMID: 24361246
    11. Inactivation of Monoacylglycerol lipase robustly suppressed production and accumulation of beta-amyloid (Abeta) associated with reduced expression of beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) in a mouse model of Alzheimer's disease. PMID: 23122958
    12. Data indicate that nerve growth factor (NGF) controls monoacylglycerol lipase (MGL) degradation in vitro and in vivo. PMID: 23319656
    13. The results therefore suggest a role for intestinal MGL in whole body energy balance via regulation of food intake as well as metabolic rate. PMID: 22937137
    14. MGL regulates 2-arachidonoylglycerol signaling rather broadly within a certain range of neural tissue, although MGL expression is heterogeneous and limited to a subset of nerve terminals and astrocytes. PMID: 22783023
    15. results indicate that genetic deletion of MAGL causes profound changes in eCB signaling, long-term synaptic plasticity, and learning behavior. PMID: 21940435
    16. pathway exists in brain where MAGL hydrolyzes 2-arachidonoylglycerol to generate arachidonate precursor pool for neuroinflammatory prostaglandins; found MAGL as metabolic node coupling endocannabinoid to prostaglandin signaling networks in nervous system PMID: 22021672
    17. MGL deficiency impairs lipolysis and attenuates diet-induced insulin resistance PMID: 21454566
    18. MAGL is the major regulator of 2- arachidonoylglycerol (2-AG) levels and signaling. PMID: 20855465
    19. spatially confined MGL activity generates a 2-arachidonoyl glycerol sensing microdomain and configures 2-arachidonoyl glycerol signaling to promote axonal growth PMID: 20962221
    20. sustained inactivation of monoacylglycerol lipase impairs specific endocannabinoid-mediated forms of synaptic plasticity; ligand diversification may be important for shaping the distinct functions and properties of endocannabinoid signaling pathways PMID: 20729846
    21. Astrocytes express monoacylglycerol lipase, the main hydrolyzing enzyme of 2-arachidonoylglycerol. Pharmacological inhibition of MGL potentiates ATP-induced 2-AG production. PMID: 15371507
    22. analysis of developmental and nutritional regulation of monoacylglycerol lipase and monoacylglycerol acyltransferase PMID: 17848545

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    AB hydrolase superfamily, Monoacylglycerol lipase family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:23945

    STRING: 10090.ENSMUSP00000127374

    UniGene: Mm.272197