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  4. Recombinant Mouse Mothers against decapentaplegic homolog 1 (Smad1)

Recombinant Mouse Mothers against decapentaplegic homolog 1 (Smad1)

  • 货号：
    CSB-YP021786MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021786MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021786MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021786MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021786MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Smad1; Madh1; Madr1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; Dwarfin-A; Dwf-A; Mothers-against-DPP-related 1; Mad-related protein 1; mMad1; SMAD family member 1; SMAD 1; Smad1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-465
  • 氨基酸序列
    MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQ PSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEV CINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPN SHPFPHSPNSSYPNSPGGSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQ PMDTNMMAPPLPAEISRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFT DPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNC NYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGW GAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
  • 基因功能参考文献：
    1. TGF-beta/Smad proteins signaling affects radiation response and prolongs survival by regulating DNA repair genes in malignant glioma. PMID: 30230914
    2. the reduced bone formation in GIO mice could not only be prevented by osteoblasts-specific Ckip-1 ablation, but also be attenuated after osteoblasts-specific Smad1 overexpression PMID: 28128304
    3. Results show that Smad1 plays an important role in the development of glomerular injury without affecting cell proliferation, in progressive glomerulonephritis. PMID: 27492138
    4. These findings uncover crosstalk between the BMP-Smad1 and RANKL-NF-kappaB pathways during osteoclastogenesis that underlies the action of active vitamin D on bone health. PMID: 28370465
    5. CD137 signaling is a new regulator of angiogenesis by modulating the Smad1/5-NFATc1 pathway. PMID: 28288971
    6. TGF-beta1-induced pSmad1 levels were elevated in Nedd4-deficient primary VSMCs isolated from Nedd4(fl/fl) ;SM22alpha-Cre mice. PMID: 28029182
    7. AA-enhanced cardiomyogenesis thus relies on the ability of AA to modulate the ratio of SMAD signaling among the TGFbeta-superfamily receptor signaling pathways PMID: 29232368
    8. Sphingosine 1 phosphate also up-regulated runt-related transcription factor 2 (Runx2) expression through S1PR2/RhoA/ROCK/Smad1/5/8 signaling. PMID: 27612439
    9. these results identify the PI3K-GSK3-SMAD1 axis as a central node integrating multiple signaling networks that govern bone formation and homeostasis. PMID: 26896753
    10. We discovered that Smad1/5/4-Amhr2-cre KO females have malformed oviducts that subsequently develop oviductal diverticuli. In addition, uteri from Smad1/5/4-Amhr2-cre KO females exhibit multiple defects in stroma, epithelium, and smooth muscle layers and fail to assemble a closed uterine lumen upon embryo implantation, with defective uterine decidualization that led to pregnancy loss at early to mid-gestation. PMID: 27335065
    11. these studies characterize an accessory TGF-beta-stimulated BMP R-Smad signaling mechanism in interstitial cells of the developing lung. PMID: 28642261
    12. these results identify a novel function of YAP in neocortical astrocytic differentiation and proliferation, and reveal a BMP2-YAP-SMAD1 pathway underlying astrocytic differentiation in the developing mouse neocortex. PMID: 27381227
    13. Dynamin-dependent endocytosis of Bone Morphogenetic Protein2 (BMP2) and its receptors is dispensable for the initiation of Smad signaling PMID: 27113717
    14. Thyroid-specific Smad1 and Smad5 double-knockout (Smad1/5(dKO)) mice displayed growth retardation, hypothyroidism and defective follicular architecture. PMID: 27068110
    15. Smad1 and Smad5 have overlapping functions to govern hepcidin transcription. Moreover, erythropoietin and erythroferrone target Smad1/5 signaling and require Smad1/5 to suppress hepcidin expression. PMID: 28438754
    16. Actin cytoskeleton depolymerization inhibites BMP2 signaling via blocking of Smad by dephosphorylated CNN1 in osteoblast cells under simulated microgravity. PMID: 28457943
    17. Store operated calcium entry negatively regulates the Smad1 signaling pathway and inhibits Col IV protein production in glomerular mesangial cells. PMID: 28298362
    18. Thus, our findings identify an unrecognized function of neogenin in mouse neocortical astrocyte differentiation, and suggest a signaling pathway, BMP2-neogenin-YAP-Smad1, underlying astrogliogenesis in developing mouse neocortex. PMID: 27225772
    19. Collectively, the data suggest that Cytl1 plays an essential role in cardiac fibrosis likely through activating the TGF-beta-SMAD signaling pathway. PMID: 27835665
    20. BetA can enhance in vivo osteogenic potentials of BMP2, possibly via stimulating Smad 1/5/8 and p38 pathways, and combination of both agents can be considered as a therapeutic strategy for bone diseases. PMID: 27188281
    21. Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1-Smad5-Smad8 phosphorylation. PMID: 27030100
    22. Regulation of impaired angiogenesis in diabetic dermal wound healing by microRNA-26a is mediated by the increased expression of its target gene, SMAD1. PMID: 26776318
    23. Data show that in R-Smad proteins Smad1;Smad5 knockout embryonic stem cells (mESCs), the bone morphogenetic proteins (BMP)-SMAD signaling is dispensable for self-renewal. PMID: 26711875
    24. AHNAK plays a crucial role in body fat accumulation by regulating adipose tissue development via interaction with the SMAD1 protein and can be involved in metabolic homeostasis PMID: 26466345
    25. Results show that Smad1-deficient mice exhibit enhanced neuronal survival and improved neurological recovery at 7 days after transient middle cerebral artery occlusion suggesting that Smad1-mediated signal pathway is involved in stroke pathophysiology. PMID: 26317208
    26. The study reveals a novel miR-26a-GSK3beta-Smad1 signaling pathway in the regulation of mammalian axon regeneration. PMID: 26313916
    27. Nondiabetic Smad1-Tg had normal kidney however, diabetes resulted in mesangial expansion, consistent with an increase in glomerular phosphorylated Smad1. Heterozygous Smad1 KO mice exhibit attenuated mesangial expansion in the diabetic condition. PMID: 25995358
    28. The findings demonstrate for the first time that KMUP-1 can promote osteoblast maturation and differentiation in vitro via BMP-2/Smad1/5/8 and Wnt/beta-catenin pathways. PMID: 25536014
    29. FLI1 and GATA2 are upstream regulators of SMAD1 and SMAD5 expression in endothelial cells. PMID: 25870111
    30. Data indicate that PDGF-AA promotes mesenchymal stem cel migration via bone morphogenetic protein 2 (BMP2)-smad proteins smad1/5/8 activation requires lysosome-mediated degradation of PDGF alpha Receptor (PDGFRalpha). PMID: 25470749
    31. some pSMAD1/5 targets, like Gata3, function specifically in transit amplifying cell lineage-progression. PMID: 25312496
    32. The effects of a soy-free diet on the risk of granulosa cell tumors in mice with conditional double knockout of Smad1 and Smad5 are reported. PMID: 25165122
    33. results demonstrate that OSM induces ECM accumulation in fibrosis-resistant BALB/c mouse lung in the absence of Th2 inflammation or TGF-beta signaling, and highlight a dichotomy of STAT3 activation versus SMAD1 suppression in this process PMID: 24933422
    34. This review will summarize on several unconventional pathways that participate in DDR with an emphasis on the BMP-Smad1 pathway, a known regulator of mouse development and bone remodeling. PMID: 24142423
    35. our data suggest a pivotal role for canonical BMP signaling demonstrating distinguished nonoverlapping function of pSmad8 with pSmad1 and pSmad5 in hfSCs regulation and hair morphogenesis but a redundant role in adult hair progenitors differentiation. PMID: 24023003
    36. Icariine has a direct stimulatory function on the differentiation of MC3T3-E1 osteoblastic cells in vitro, which may be mediated by increasing the production of Smad1 and Smad5 in osteoblasts PMID: 24297369
    37. PI3K-GSK3-Smad1 signalling is a central module for promoting sensory axon regeneration in the mammalian nervous system PMID: 24162165
    38. Glucocorticoids recruit Tgfbr3 and Smad1 to shift transforming growth factor-beta signaling from the Tgfbr1/Smad2/3 axis to the Acvrl1/Smad1 axis in lung fibroblasts. PMID: 24347165
    39. these data suggest that SERTAD1 acts as a SMAD1 transcriptional co-activator to promote the expression of BMP target genes during mouse cardiogenesis. PMID: 24211589
    40. Data show that HtrA1 is produced during osteoclastogenesis, and negatively regulates osteoblast differentiation, osteoblast differentiation and BMP2-induced Smad1/5/8, ERK1/2 and p38 phosphorylation in pre-osteoblasts. PMID: 24269886
    41. Data indicate that smad1 upregulation occurs at the level of protein stabilization, and the upregulated Smad1 was relocalized to the perinuclear region. PMID: 23793844
    42. FGF2 inhibited BMP9-induced osteogenic differentiation by blocking BMP9-induced Smads signaling and subsequently reducing Smads dependent up-regulation of ALK1 and ALK2 in mesenchymal stem cells. PMID: 23680673
    43. a detailed computational model for TGF-beta signalling that incorporates elements of previous models together with crosstalking between Smad1/5/8 and Smad2/3 channels through a negative feedback loop dependent on Smad7. PMID: 23804438
    44. BMP4-mediated signaling in adult mouse ovary-derived oogonial stem cells cultured in vitro drives differentiation of these cells into oocytes through Smad1/5/8 activation and transcriptional up-regulation of key meiosis-initiating genes. PMID: 23993924
    45. The carboxy-terminal phosphorylation Smad1C Mediated Erk1/2 Transcription Sensitizes Sensory Neurons to Neurotrophins. PMID: 23665221
    46. our studies establish that loss of SMAD1/5 leads to upregulation of PDGFA in ovarian granulosa cells PMID: 22964636
    47. Endogenous FGF2 is necessary for BMP-2 induced nuclear accumulation and co-localization of Runx-2 and phospho-Smads1/5/8. PMID: 23559326
    48. BMP2-triggered receptor kinase activity drives Smad1 mediated long-term target gene oscillation in mouse myoblasts. PMID: 23560048
    49. SMAD1 deficiency in either endothelial or smooth muscle cells can predispose mice to pulmonary hypertension. PMID: 23478097
    50. Smad1/5 signaling is required for the specification process in anterior rhombic lip but not for the subsequent external granular layer development. PMID: 23459943

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:17125

    STRING: 10090.ENSMUSP00000071035

    UniGene: Mm.223717