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  4. Recombinant Mouse Myocyte-specific enhancer factor 2D (Mef2d)

Recombinant Mouse Myocyte-specific enhancer factor 2D (Mef2d)

  • 货号：
    CSB-YP733979MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP733979MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP733979MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP733979MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP733979MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mef2dMyocyte-specific enhancer factor 2D
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-514
  • 氨基酸序列
    MGRKKIQIQR ITDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNH SNKLFQYAST DMDKVLLKYT EYNEPHESRT NADIIETLRK KGFNGCDSPE PDGEDSLEQS PLLEDKYRRA SEELDGLFRR YGSSVPAPNF AMPVTVPVSN QSSMQFSNPS SSLVTPSLVT SSLTDPRLLS PQQPALQRNS VSPGLPQRPA SAGAMLGGDL NSANGACPSP VGNGYVSARA SPGLLPVANG NSLNKVIPAK SPPPPTHNTQ LGAPSRKPDL RVITSQGGKG LMHHLTEDHL DLNNAQRLGV SQSTHSLTTP VVSVATPSLL SQGLPFSSMP TAYNTDYQLP SAELSSLPAF SSPAGLALGN VTAWQQPQPP QQPQPPQPPQ SQPQPPQPQP QQPPQQQPHL VPVSLSNLIP GSPLPHVGAA LTVTTHPHIS IKSEPVSPSR ERSPAPPPPA VFPAARPEPG EGLSSPAGGS YETGDRDDGR GDFGPTLGLL RPAPEPEAEG SAVKRMRLDT WTLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • 基因功能参考文献：
    1. In the Mef2d heterozygous retina, NRF2 is not up-regulated to a normal degree in the face of light-induced oxidative stress, contributing to accelerated photoreceptor cell death. PMID: 28461502
    2. This study identifies MEF2D as a critical regulator of IL-10 gene expression that negatively controls microglia inflammation response and prevents inflammation-mediated cytotoxicity. PMID: 25890150
    3. Mef2d is essential for the maturation and integrity of retinal photoreceptor and bipolar cells PMID: 25757744
    4. miR-103 worked through activating AKT/mTOR signal pathway and impairing target gene MEF2D. PMID: 25400071
    5. These findings demonstrate that broadly expressed TFs acquire specific functions through competitive recruitment to enhancers by tissue-specific Mef2d and through selective activation of these enhancers to regulate tissue-specific genes. PMID: 25801704
    6. Oxidation of survival factor MEF2D inhibits its function, underlies oxidative stress-induced neurotoxicity, and may be a part of the Parkinson disease pathogenic process. PMID: 24219011
    7. Results demonstrate that coordinated alternative splicing by a single RNA binding protein modulates transcription (Mef2d) and cell signaling (Rock2) programs to drive tissue-specific functions (cell fusion) to promote a developmental transition. PMID: 25087874
    8. We found that Parkinson's disease -associated neurotoxins destabilize MEF2D mRNA and reduce its level in vitro and in vivo. PMID: 24848448
    9. The resultsof this study showed that MEF2D is a substrate for phosphorylation by ATM, thus promoting survival in response to DNA damage. PMID: 24672010
    10. This study identified lysine-267 as a methylation/demethylation site and demonstrated that the lysine methylation state of MEF2D regulates its transcriptional activity and skeletal muscle cell differentiation. PMID: 24078251
    11. results reveal a novel mechanism in which a tissue-specific alternate splicing event has evolved that permits a ubiquitously expressed transcription factor, Mef2d, to escape inhibitory signaling for temporal regulation of gene expression PMID: 23723416
    12. the inactivation of Nur77, induced by loss of MEF2 activity, plays a critical role in nigrostriatal degeneration in vivo PMID: 23536182
    13. Expression of myogenin is regulated, at least in part, by the decreased glycosylation-dependent recruitment of Mef2D to the promoter region, and this is one of the negative regulatory mechanisms of skeletal myogenesis by O-GlcNAc glycosylation. PMID: 23523791
    14. Activation of transcription factor MEF2D by bis(3)-cognitin protects dopaminergic neurons and ameliorates Parkinsonian motor defects. PMID: 22891246
    15. direct regulation of complex I by mitochondrial MEF2D underlies its neuroprotective effects, and dysregulation of this pathway may contribute to Parkinson disease PMID: 21393861
    16. IGF-I blocks the degradation of MEF2D and enhances recovery of MEF2 activity by protecting MEF2D from caspase-dependent cleavage during apoptosis. PMID: 14710356
    17. These results demonstrate the importance of the Emb-MEF2D-histone transacetylase p300 complex in the transcriptional regulation of the cardiac actin gene and suggest a potential role in chromatin remodeling associated with muscle gene activation. PMID: 15024082
    18. histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications regulate MEF2 PMID: 16166628
    19. MEF2 is critical for dopaminergic loss because "cdk5 phosphorylation site mutant" of MEF2D provides neuroprotection in an MPTP mouse model of Parkinson disease. Calpain-p35-p25/cdk5-mediated inactivation of MEF2 plays a critical role in this loss in vivo. PMID: 16407541
    20. myogenin specifies the muscle phenotype by cooperating with Mef2D to recruit an ATP-dependent chromatin-remodeling enzyme that alters chromatin structure at regulatory sequences to promote terminal differentiation. PMID: 16424906
    21. These findings demonstrate calcium-ionophore-induced activation of the beta MyHC promoter by NFATc1, MyoD, MEF-2D, and p300 in a calcineurin-dependent manner. PMID: 17111365
    22. These results reveal an unexpected role for HDAC3 and suggest a novel pathway through which MEF2 activity is controlled in vivo. PMID: 17158926
    23. a unique and important function for MEF2D in stress-dependent cardiac growth and reprogramming of gene expression in the adult heart. PMID: 18079970
    24. MEF2D is a primary target of PKA signaling in myoblasts that leads to inhibition of the skeletal muscle differentiation program. PMID: 18299387
    25. study found chaperone-mediated autophagy regulated the activity of MEF2D; MEF2D was observed to shuttle to the cytoplasm, interact with the chaperone Hsc70 & undergo degradation; MEF2D levels were increased in brains of alpha-synuclein transgenic mice PMID: 19119233

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    MEF2 family
  • 组织特异性：
    Widely expressed though mainly restricted to skeletal and cardiac muscle, brain, neurons and lymphocytes. Differentially expressed depending on if isoforms contain the beta domain or not, with the total expression of the beta domain-lacking isoforms vastl
  • 数据库链接：

    KEGG: mmu:17261

    STRING: 10090.ENSMUSP00000001455

    UniGene: Mm.28184