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  4. Recombinant Mouse Myristoylated alanine-rich C-kinase substrate (Marcks)

Recombinant Mouse Myristoylated alanine-rich C-kinase substrate (Marcks)

  • 中文名称：
    小鼠Marcks重组蛋白
  • 货号：
    CSB-YP013493MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Marcks重组蛋白
  • 货号：
    CSB-EP013493MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Marcks重组蛋白
  • 货号：
    CSB-EP013493MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Marcks重组蛋白
  • 货号：
    CSB-BP013493MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Marcks重组蛋白
  • 货号：
    CSB-MP013493MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Marcks; MacsMyristoylated alanine-rich C-kinase substrate; MARCKS
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-309
  • 氨基酸序列
    GAQFSKTAA KGEATAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA EPGAKEELQA NGSAPAADKE EPASGSAATP AAAEKDEAAA ATEPGAGAAD KEAAEAEPAE PSSPAAEAEG ASASSTSSPK AEDGAAPSPS SETPKKKKKR FSFKKSFKLS GFSFKKSKKE SGEGAEAEGA TAEGAKDEAA AAAGGEGAAA PGEQAGGAGA EGAAGGEPRE AEAAEPEQPE QPEQPAAEEP QAEEQSEAAG EKAEEPAPGA TAGDASSAAG PEQEAPAATD EAAASAAPAA SPEPQPECSP EAPPAPTAE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 基因功能参考文献：
    1. this work establishes a novel role for MARCKS in axon dynamics and highlights the necessity of MARCKS as an organizer of DCC signaling at the membrane. PMID: 29546593
    2. Strongly support the existence of a PKC-MARCKS-PI3K regulatory module. PMID: 29715315
    3. ENaC activity is regulated by calpain-2 proteolysis of MARCKS. PMID: 28468944
    4. In the absence of Pin1, MARCKS is hyper-phosphorylated, leading to loss of cell adhesions, and collapse of the growth cone. PMID: 26991250
    5. Findings suggest that MIR429 modulates mucin secretion in human colorectal cells and mouse colitis tissues by up-regulating of MARCKS expression. PMID: 26818658
    6. Conditional deletion of MARCKS in ECs induces intracellular accumulation of mucins, elevated oxidative stress, and lipid droplet buildup. PMID: 26010231
    7. MARCKS acts as a "molecular switch," binding to and regulating PIP2 signaling to regulate processes like proplatelet extension (microtubule-driven) vs proplatelet branching (Arp2/3 and actin polymerization-driven). PMID: 26744461
    8. MARCKS knockdown arrested VSMC cell cycle by decreasing KIS expression. Decreased KIS expression resulted in nuclear trapping of p27kip1 in VSMCs. PMID: 26528715
    9. MARCKS regulates the expression of proinflammatory cytokines in macrophages through activation of p38/JNK MAPK and NF-kappaB. PMID: 25929183
    10. Targeting phospho-MARCKS overcomes drug-resistance and induces antitumor activity in preclinical models of multiple myeloma. PMID: 25179733
    11. Grin 1 is identified as a novel Cdk5 substrate and MARCKS is confirmed as a Cdk5 substrate. PMID: 24658276
    12. MARCKS appears to be a nonessential regulatory protein in mast cell exocytosis but exerts a negative modulation. PMID: 24705720
    13. MARCKS overexpression in the hippocampus of old mice leads to long-term potentiation and improved memory. PMID: 23434911
    14. an important functional role for the interaction between MARCKS and PSA in the developing and adult nervous system. PMID: 23329829
    15. cathepsin K exocytosis is controlled by PKCdelta through modulation of the actin bundling protein myristoylated alanine-rich C-kinase substrate. PMID: 22806935
    16. These results suggest that MARCKS is involved in directed migration of macrophages PMID: 22623357
    17. a critical role for H(2)O(2) in angiotensin-II signaling to the endothelial cytoskeleton in a novel pathway that is critically dependent on MARCKS, Rac1, and c-Abl. PMID: 22773836
    18. the capacity of MARCKS-ED to regulate granule exocytosis in a PKC-dependent manner, consistent with regulated sequestration of phosphoinositides that mediate granule fusion at the plasma membrane. PMID: 22013076
    19. MARCKS contributes to the negative regulation of the cellular response to LPS. PMID: 22427633
    20. L-PGDS protein regulates glial cell migration and morphology through Marcks in prostaglandin D2 independent manner. PMID: 22275363
    21. findings suggest that abnormal expression of MARCKS via dysfunction of Dysbindin might cause impairment of neural transmission and abnormal synaptogenesis PMID: 20098743
    22. Motif analyses revealed ca. 12 overlapping potential transcription factor binding sites; synergistic interaction between distal and proximal promoter sequences is indispensable for the optimal MARCKS promoter function PMID: 11944910
    23. Sequential activation of individual PKC isozymes in integrin-mediated muscle cell spreading: a role for MARCKS in an integrin signaling pathway. PMID: 11973356
    24. Hippocampal, total MARCKS mRNA and protein levels in C57BL/6J mice were significantly lower compared with DBA/2J mice; Restraint stress resulted in significant reduction in membrane MARCKS expression in C57BL/6J hippocampus but not for DBA/2J mice PMID: 12675922
    25. post-translational modifications of MARCKS are necessary and sufficient to regulate actin-binding activity PMID: 15640140
    26. Results show that phosphorylated MARCKS is a novel centrosome component and also defines a previously unrecognized subdomain of the actin cap overlying the spindle. PMID: 15766745
    27. Antisense strategy directed against MARCKS, a calpain substrate during C2C12 migration, showed that this protein could play a role in stress fiber polymerization. PMID: 16496301
    28. Increased cleavage of MARCKS in brain and macrophages, but not in liver and kidney extracts of stefin B-deficient mice compared to wild-type mice, was observed. PMID: 17655504
    29. Data demonstrate in mice that the myristoylated alanine-rich C-kinase substrate protein (MARCKS), a prominent cellular substrate for PKC, modulates radial glial placement and expansion. PMID: 19666823

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Membrane; Lipid-anchor.
  • 蛋白家族：
    MARCKS family
  • 组织特异性：
    Brain, spleen, less in kidney and heart, and very low levels in liver.
  • 数据库链接：

    KEGG: mmu:17118

    STRING: 10090.ENSMUSP00000090245

    UniGene: Mm.30059