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  4. Recombinant Mouse NAD-dependent protein deacetylase sirtuin-6 (Sirt6)

Recombinant Mouse NAD-dependent protein deacetylase sirtuin-6 (Sirt6)

  • 货号：
    CSB-YP021344MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021344MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021344MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021344MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021344MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sirt6; Sir2l6; NAD-dependent protein deacetylase sirtuin-6; EC 2.3.1.286; Regulatory protein SIR2 homolog 6; SIR2-like protein 6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-334
  • 氨基酸序列
    MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLMWQSSSVVFHTGAGISTASG IPDFRGPHGVWTMEERGLAPKFDTTFENARPSKTHMALVQLERMGFLSFLVSQNVDGLHV RSGFPRDKLAELHGNMFVEECPKCKTQYVRDTVVGTMGLKATGRLCTVAKTRGLRACRGE LRDTILDWEDSLPDRDLMLADEASRTADLSVTLGTSLQIRPSGNLPLATKRRGGRLVIVN LQPTKHDRQADLRIHGYVDEVMCRLMKHLGLEIPAWDGPCVLDKALPPLPRPVALKAEPP VHLNGAVHVSYKSKPNSPILHRPPKRVKTEAAPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NAD-dependent protein deacetylase involved in various processes including telomere maintenance and gene expression, and consequently has roles in genomic stability, cell senescence and apoptosis. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. May also be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. Acts as a corepressor of the transcription factor Hif1a to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for normal IGF1 serum levels and normal glucose homeostasis. Regulates the production of TNF protein. Has a role in the regulation of life span in male mice, but not in female mice.
  • 基因功能参考文献：
    1. therapeutic effect on periapical lesions through suppression of CCL2 synthesis PMID: 28039864
    2. Study provides evidence that sirtuin 6 (SIRT6) can protect the brain from cerebral cerebral ischemia/reperfusion injury by suppressing oxidative stress via nuclear factor (erythroid-derived 2)-like 2 activation. Thus, SIRT6 may serve as a potential therapeutic target for ischemic stroke. PMID: 28951325
    3. SIRT6 knockout cells resist apoptosis initiation but not progression. PMID: 28856570
    4. Sirt6 in pancreatic beta cells deacetylates FoxO1 and subsequently increases the expression of Pdx1 and Glut2 to maintain the glucose-sensing ability of pancreatic beta cells and systemic glucose tolerance. PMID: 27457971
    5. SIRT6 interacts with and promotes phospho-ATF2 binding to the PGC-1alpha gene promoter to activate its expression. The present study reveals a critical role for SIRT6 in regulating thermogenesis of fat. PMID: 28723567
    6. Moreover, the s showed that Sirt6, induced by RANKL-dependent NFATc1 expression, forms a complex with B lymphocyte-induced maturation protein-1 (Blimp1) to negatively regulate expression of anti-osteoclastogenic gene such as Mafb. PMID: 27189179
    7. Collectively, our findings uncover the autophagy modulated by SIRT6 may be a proinflammatory mechanism for amyloid-beta induced RPE dysfunction. PMID: 29402409
    8. Sirt6 inhibits Notch1 and Notch4 transcription by deacetylating histone H3K9. PMID: 28871079
    9. Sirt6 is downregulated in induced pluripotent stem cells from old mice. PMID: 28329681
    10. SIRT6 and its downstream signaling could be targeted in Alzheimer's disease and age-related neurodegeneration. PMID: 28355558
    11. These findings reveal a crucial role of SIRT6 in adipogenesis and provide potential therapeutic targets for obesity. PMID: 28355567
    12. Global and endothelium-specific SIRT6 knockout mice exhibited impaired endothelium-dependent vasorelaxation. Moreover, SIRT6(+/-) haploinsufficient mice fed a high-fat diet (HFD) also displayed impaired endothelium-dependent vasorelaxation. Importantly, SIRT6(+/-); ApoE(-/-) mice after HFD feeding had exacerbated atherosclerotic lesion development, concurrent with increased expression of proinflammatory cytokine VCAM-1. PMID: 27249230
    13. these data provide compelling evidence that Sirt6 is important for podocyte homeostasis and maintenance of glomerular function PMID: 28351995
    14. The mice with SIRT6 deficiency in muscle displayed impaired glucose homeostasis and insulin sensitivity, attenuated whole body energy expenditure, and weakened exercise performance. Results from both gain- and loss-of-function experiments identify SIRT6 as a physiological regulator of muscle mitochondrial function. PMID: 28765271
    15. Study investigated the involvement of SIRT6 in adult hippocampal neurogenesis. Mice over-expressing Sirt6 exhibit increased numbers of young neurons and decreased numbers of mature neurons, without affecting glial differentiation. PMID: 28644902
    16. Sirt6 deletion in macrophages promoted the activation of nuclear factor-kappaB (NF-kappaB) and endogenous production of interleukin-6, which led to STAT3 activation and the positive feedback circuits for NF-kappaB stimulation; this cross talk expedited an M1 polarization PMID: 28607107
    17. These data suggest that the function of SIRT6 in the Fabp4-Cre-expressing cells in addition to mature adipocytes plays a critical role in body weight maintenance and metabolic homeostasis. PMID: 28385723
    18. a physiological defects in adult SIRT6-/- mice PMID: 28448551
    19. In mouse retinae lacking Sirt6, effectors of glycolytic flux were dramatically increased, leading to upregulation of key intermediates in glycolysis, TCA cycle, and glutaminolysis. PMID: 27841758
    20. It has been demonstrated that Sirt6 plays a role in tooth root formation, and it was confirmed that SIRT6 is necessary for dental mesenchymal cells differentiation as well as for the development of the tooth root and for eventual tooth eruption. PMID: 28355287
    21. Fat-specific Sirt6 knockout (FKO) sensitized mice to high-fat diet-induced obesity, which was attributed to adipocyte hypertrophy rather than adipocyte hyperplasia. Fat-specific Sirt6 KO also increased inflammation in the adipose tissue, which may have contributed to insulin resistance in high-fat diet-fed FKO mice. PMID: 28250020
    22. SIRT6 is a primary negative regulation factor in endothelial dysfunction and atherosclerosis development. PMID: 26924042
    23. Data suggest that expression of Sirt6 in pancreatic beta cells protects against dysfunction (loss of hyperglycemia-induced insulin secretion) and apoptosis; Sirt6 appears to protect beta cells against the oxidative stress of diabetes type 2 and of cellular aging. PMID: 27601447
    24. SIRT6, a sirtuin with established tumor suppressor function, regulates the lysine fatty acylation of R-Ras2. PMID: 28406396
    25. data indicate that distinct activities of SIRT6 regulate different pathways and that the G60A mutant is a useful tool to study the contribution of defatty-acylase activity to SIRT6's various functions PMID: 27322069
    26. The critical role of SIRT6 on aging and inflammation. PMID: 26940461
    27. The SIRT6 regulates HSC homeostasis through the transcriptional repression of Wnt target genes. Sirt6 deletion promoted HSC proliferation through aberrant activation of Wnt signaling. PMID: 27058938
    28. Sirt6 plays a crucial role in dendritic cell differentiation and function and reduced Sirt6 activity may contribute to immunosenescence. PMID: 26761436
    29. up-regulates AMP/ATP and then activates the AMPK-FoxO3alpha axis and further initiates the downstream antioxidant-encoding gene expression, thereby decreasing cellular levels of oxidative stress and mediating cardioprotection in the ischemic heart PMID: 26786260
    30. SIRT6 is important for glucose-stimulated insulin secretion from pancreatic beta cells and activation of SIRT6 may be useful to improve insulin secretion in diabetes PMID: 26471901
    31. These data strongly suggest that overexpression of Sirt6 can prevent osteoarthritis development by reducing both the inflammatory response and chondrocytes senescence. PMID: 26639398
    32. Sirt6 depletion causes spindle defects and chromosome misalignment during meiosis of mouse oocyte PMID: 26481302
    33. In SIRT6-deficient mice there is impaired bone remodeling leading to osteopenia. PMID: 26189760
    34. SIRT6/NF-kappaB signaling axis in ginsenoside Rg1-delayed hematopoietic stem/progenitor cell senescence. PMID: 26191269
    35. Data showed the expression levels of SIRT1, SIRT3, and SIRT6 were decreased in the ovaries of aged mice and mice treated with chemotherapy, but increased in mice treated to caloric restriction suggesting these genes are closely related to ovarian reserve. PMID: 26403315
    36. Sodium sulfide protected brain endothelial cells against simulated ischemic injury through SIRT6-dependent mechanisms. PMID: 25543188
    37. SIRT1 localizes to the promoters of several aberrantly silenced tumor suppressor genes whose DNA is hypermethylated PMID: 26775341
    38. Sirt6 has key roles in the relationship among ageing, metabolic syndrome, and osteoarthritis PMID: 26362183
    39. Overall these results suggest that SIRT6 may play a role in synaptic function and neuronal maturation and PMID: 25366464
    40. Studied the effect of icariin (ICA) on cardiac aging through its effects on the SIRT6 enzyme and on the NF-kappaB pathway. PMID: 25688369
    41. Our results identify SIRT6 as a critical modulator of retinal function, likely through its effects on chromatin. PMID: 24896097
    42. SIRT6 represses LINE1 retrotransposons by ribosylating KAP1 but this repression fails with stress and age. PMID: 25247314
    43. SIRT6 functions as a chromatin regulator safeguarding the balance between pluripotency and differentiation through Tet-mediated production of 5-hydroxymethylcytosine. PMID: 25915124
    44. Mouse sirtuin 6 immunohistochemical changes in the early stages of type II Diabetes mellitus. PMID: 24063863
    45. Over-expression of SIRT6 protects cardiomyocytes against hypoxia. PMID: 25066211
    46. SIRT6 regulates the cigarette smoke-induced signalling in rheumatoid arthritis synovial fibroblasts PMID: 24638860
    47. Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling. PMID: 24149372
    48. Our findings uncover a novel, evolutionary conserved function of SIRT6 in the maintenance of stress granules in response to stress. PMID: 24013546
    49. In normal aging cell metabolism is reduced, in the diabetic mice a compensatory mechanism may elevate Sirt1 and Sirt6 levels. PMID: 23761075
    50. CHIP noncanonically ubiquitinates SirT6 at K170, which stabilizes SirT6 and prevents SirT6 canonical ubiquitination by other ubiquitin ligases. PMID: 24043303

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  • 亚细胞定位：
    Nucleus. Note=Predominantly nuclear. Associated with chromatin.
  • 蛋白家族：
    Sirtuin family, Class IV subfamily
  • 组织特异性：
    Highest levels are found in muscle, thymus, spleen, brain and heart (at protein level).
  • 数据库链接：

    KEGG: mmu:50721

    STRING: 10090.ENSMUSP00000048971

    UniGene: Mm.131825