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  4. Recombinant Mouse Natural resistance-associated macrophage protein 2 (Slc11a2), partial

Recombinant Mouse Natural resistance-associated macrophage protein 2 (Slc11a2), partial

  • 中文名称：
    小鼠Slc11a2重组蛋白
  • 货号：
    CSB-YP021381MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Slc11a2重组蛋白
  • 货号：
    CSB-EP021381MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Slc11a2重组蛋白
  • 货号：
    CSB-EP021381MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Slc11a2重组蛋白
  • 货号：
    CSB-BP021381MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Slc11a2重组蛋白
  • 货号：
    CSB-MP021381MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc11a2
  • Uniprot No.：
  • 别名：
    Slc11a2; Dct1; Dmt1; Nramp2; Natural resistance-associated macrophage protein 2; NRAMP 2; Divalent cation transporter 1; Divalent metal transporter 1; DMT-1; Solute carrier family 11 member 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May serve to import iron into the mitochondria. Important in metal transport, in particular iron. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • 基因功能参考文献：
    1. the cellular iron importer, divalent metal transporter 1 (DMT1), is highly expressed in colorectal cancer through hypoxia-inducible factor 2alpha-dependent transcription. PMID: 27546461
    2. Ndfip2 controls DMT1 in the liver with female mice showing a greater response to altered dietary iron than the male mice PMID: 27048792
    3. PrP(C) promotes, and possibly regulates, the uptake of iron through DMT1 and Zip14 via its ferrireductase activity. PMID: 25862412
    4. This study reveals a critical function for DMT1 in intestinal nonheme-iron absorption for normal growth and development, and that it is not required for the transport of copper, manganese, or zinc. PMID: 26294671
    5. We propose that DMT1 deficiency negatively affects metabolism and life span of mature erythrocytes; two other aspects of defective erythropoiesis which contribute to the pathophysiology of the disease PMID: 25562168
    6. Misregulation of DMT1 is implicated in Parkinson's disease. PMID: 25467637
    7. Suggest role for divalent cation transporter DMT1 in the entry of Hg(II) into the intestinal epithelium. PMID: 25772431
    8. The Cu uptake in HEK293 cells that over-expressed the 2/-IRE isoform of DMT1 transporter supports our earlier contention that DMT1 transports Cu as Cu(1+). PMID: 24327293
    9. Hepatocyte divalent metal-ion transporter-1 is dispensable for hepatic iron accumulation and non-transferrin-bound iron uptake in mice. PMID: 23508576
    10. DMT-1 expression in hypothalamic subregions is cre recombinase sensitive, and that central-peripheral pathways may impact beta cell function in the Dmt1 null mouse. PMID: 23000401
    11. CD61 regulates the expression of Slc11a2, which is involved in iron transportation in epithelial tissues. PMID: 23064962
    12. that 1B/(-)IRE DMT1 expression and intracellular iron influx are early downstream responses to NF-kappaB/RelA activation and acetylation during brain ischemia and contribute to the pathogenesis of stroke-induced neuronal damage PMID: 22666436
    13. The knockdown of ZIP8, ZIP14 or DMT1 by siRNA transfection significantly reduced the uptake of Cd(2+) and Mn(2+) from the apical membrane. PMID: 22534978
    14. in b/b rats depressed liver hepcidin production and activated intestinal Hif2alpha starting at the C-pole resulted in increasing expression of iron transport genes, including DMT1 G185R, in an attempt to compensate for the anemia in Belgrade rats. PMID: 21436314
    15. These data demonstrate that Ndfip1 is a critical mediator of DMT1 regulation in vivo, particularly under iron restricted conditions PMID: 20959604
    16. Data suggest that reduced iron release from duodenal cells into circulation due to suppression of SLC11A2 plays a role in mitigating excessive iron uptake from the diet PMID: 20558581
    17. Results suggest that divalent metal transporter 1 (DMT1) participates in inflammatory lung injury after bleomycin with DMT1 knockout mice having increased inflammation and damage following exposure. PMID: 20336479
    18. IRE-mediated stabilization of isoform I mRNA is thought to stimulate DMT1 expression in response to iron deficiency. PMID: 11964145
    19. DMT1 may act as a re-uptake system for divalent cations at the brush border of kidney proximal tubules. A pathological mutation at DMT1 affects targeting/expression of the protein in the kidney. PMID: 12081573
    20. iron regulation of DMT1 involves the expression of a previously unrecognized upstream 5' exon (exon 1A) of the human and murine DMT1 gene PMID: 12209011
    21. associated with the membrane of phagosomes in macrophages and Sertoli cells PMID: 12239176
    22. 3 negatively charged, highly conserved residues in transmembrane domains 1, 4, & 7 are essential for DMT1 cation transport. His267/His272 are not involved in metal binding; they play an important role in pH regulation of metal transport by Nramp proteins. PMID: 12522007
    23. Increased expression of -IRE DMT1 in the lungs of the hypotransferrinemic mice could be responsible for elevated concentrations of the metal in these tissues. PMID: 12576298
    24. the divalent metal influx transporter DMT1 is expressed by astrocytes and is likely to mediate iron influx into these glial cells PMID: 12743117
    25. Embryonic fibroblasts derived from Npcis mice carry a hypomorphic allele of the DNA methyltransferase and have a 2-fold increase in LOH. PMID: 12776125
    26. Results show no correlation between the expression levels of the duodenal iron transporters DMT1 and IREG1 and the liver iron content in Hfe (hemochromatosis) knockout mice. PMID: 14618243
    27. comparison of the developmental, regional, and cellular distribution of SFT/UbcH5A and DMT1 mRNA in the neonatal and adult mouse brain PMID: 15139022
    28. Dcytb, DMT1, Ireg1 and transferrin receptor 1 have roles in iron transport and hemolysis PMID: 15469906
    29. Iron absorption genes(Dcytb,IREG1,DMT1)were expressed at lower levels in mouse caecum and colon than in the duodenum. They are regulated by body iron requirements. PMID: 15764147
    30. not needed for materno-fetal iron transfer but essential for intestinal non-heme iron absorption after birth PMID: 15849611
    31. site directed mutants at position (E399D, E399Q, E399A) in mice DMT1 are shown to be fully functional with respect to stability, targeting and trafficking to the membrane, and are transport-competent. PMID: 16023393
    32. major signal for internalization and recycling of DMT1 isoform II (-IRE/1B) resides in its carboxyl terminus and that removal of this signal leads to a default lysosomal targeting PMID: 16142913
    33. Targeting & trafficking properties of DMT1 isoforms I & II studied cells. Isoform I showed higher surface expression & was internalized from plasma membrane with slower kinetics than II. I was not efficiently recycled & was targeted to lysosomes. PMID: 16475818
    34. These data demonstrate that binding of NF-Y to this CCAAT-box domain is responsible for the basal regulation of 1B isoforms of DMT1 mRNA. PMID: 17262811
    35. DMT1 is not the sole transporter of intestinal cadmium absorption in vivo. PMID: 18076961
    36. Data show that the expression of divalent metal transporter 1 (DMT1) mRNA and protein was controlled by IRPs. PMID: 18177727
    37. in hemolytic anemia, renal Prohepcidin acts in coordination with renal Ferroportin and DMT1, indicating the key involvement of kidney in iron homeostasis when iron demand is high PMID: 18653481
    38. DMT1 interacts with 2 WW domain-interacting proteins, Ndfip1 and Ndfip2. This promotes DMT1 ubiquitination and degradation by WWP2. PMID: 18776082
    39. DMT1 expression increases in the ventral mesencephalon of intoxicated animals, concomitant with iron accumulation, oxidative stress, and dopaminergic cell loss PMID: 19011085

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  • 相关疾病：
    Defects in Slc11a2 are the cause of microcytic anemia (mk). Homozygous mk/mk mice have hypochromic microcytic anemia due to severe defects in intestinal iron absorption and erythroid iron utilization.
  • 亚细胞定位：
    Endosome membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    NRAMP family
  • 组织特异性：
    Isoform 2 is abundantly expressed in erythroid precursor cells (at protein level). Expressed at low levels in most tissues analyzed. Expressed at low levels in small intestine and at higher levels in kidney.
  • 数据库链接：

    KEGG: mmu:18174

    STRING: 10090.ENSMUSP00000023774

    UniGene: Mm.234608