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  4. Recombinant Mouse Nephronectin (Npnt)

Recombinant Mouse Nephronectin (Npnt)

  • 货号：
    CSB-YP016019MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016019MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016019MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016019MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016019MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Npnt
  • Uniprot No.：
  • 别名：
    Npnt; Neph1; Poem; Nephronectin; Preosteoblast EGF-like repeat protein with MAM domain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-561
  • 氨基酸序列
    D FDGRWPRQIV SSIGLCRYGG RIDCCWGWAR QSWGQCQPVC QPQCKHGECV GPNKCKCHPG FAGKTCNQDL NECGLKPRPC KHRCMNTFGS YKCYCLNGYM LLPDGSCSSA LSCSMANCQY GCDVVKGQVR CQCPSPGLQL APDGRTCVDI DECATGRVSC PRFRQCVNTF GSYICKCHTG FDLMYIGGKY QCHDIDECSL GQHQCSSYAR CYNIHGSYKC QCRDGYEGDG LNCVYIPKVM IEPSGPIHMP ERNGTISKGD GGHANRIPDA GSTRWPLKTP YIPPVITNRP TSKPTTRPTP NPTPQPTPPP PPPLPTEPRT TPLPPTPERP STRPTTIAPA TSTTTRVITV DNRIQTDPQK PRGDVFIPRQ PTNDLFEIFE IERGVSADEE VKDDPGILIH SCNFDHGLCG WIREKDSDLH WETARDPAGG QYLTVSAAKA PGGKAARLVL RLGHLMHSGD LCLSFRHKVT GLHSGTLQVF VRKHGTHGAA LWGRNGGHGW RQTQITLRGA DVKSVIFKGE KRRGHTGEIG LDDVSLKRGR C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.
  • 基因功能参考文献：
    1. Results show that Npnt is highly expressed in tooth germ, and localized in the basement membrane between dental epithelium and mesenchyme. The study indicates that Npnt plays a critical scaffold role in dental epithelial stem cell differentiation and proliferation, and regulates Sox2 expression during tooth development. PMID: 28345658
    2. Low Nephronectin expression is associated with Metastatic Breast Carcinoma. PMID: 28842827
    3. NPNT stimulates the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 mitogen-activated kinase (MAPK) in endothelial cells. NPNT is a paracrine angiogenic factor and may play a role in pathological osteoporosis. PMID: 27782206
    4. These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression PMID: 28093227
    5. we found that bone morphogenic protein-2 (BMP-2), known as an osteogenesis inducing cytokine, strongly up-regulated the expression of Npnt mRNA in a murine skeletal muscle cell line (C2C12) via the BMP-SMAD signaling pathway PMID: 27374296
    6. Nephronectin is capable of binding to heparan sulfate proteoglycans in basement membranes via the MAM domain. PMID: 23357641
    7. These results indicate that Npnt plays an important role in the initiation of hepatitis by recruiting CD4(+) T cells or NKT cells into the foci of inflammation. PMID: 23206711
    8. canonical TGF-b signaling through Smad2 regulates Npnt geneexpression in osteoblasts. PMID: 22842459
    9. expression of a fragment containing the 3'-untranslated region (3'-UTR) of nephronectin in osteoblast progenitor cells MC3T3-E1 promoted cell differentiation PMID: 21784074
    10. These results suggest that TNF-alpha inhibits POEM expression through the NF-kappaB signaling pathway and down-regulation of POEM influences the inhibition of osteoblast differentiation by TNF-alpha. PMID: 21689636
    11. TGF-beta inhibited differentiation of osteoblasts transfected with various truncated Npnt constructs, suggesting that TGF-beta can exert a down-stream effect on Npnt function. PMID: 20452350
    12. it was demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. PMID: 19944102
    13. results thus place nephronectin and alpha8beta1 integrin in a pathway that regulates Gdnf expression and is essential for kidney development PMID: 17537792
    14. deregulation of Nephronectin expression plays a role in the development or progression of malignant melanoma. PMID: 18271919
    15. These results indicate that the specific high-affinity binding of nephronectin to alpha8beta1 integrin is achieved by bipartite interaction of the integrin with the RGD motif and LFEIFEIER sequence. PMID: 19342381

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Note=Trapped on the cell surface or in the extracellular matrix.
  • 蛋白家族：
    Nephronectin family
  • 组织特异性：
    Expressed in kidney (at protein level).
  • 数据库链接：

    KEGG: mmu:114249

    STRING: 10090.ENSMUSP00000040071

    UniGene: Mm.279310