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  4. Recombinant Mouse Neurogenic differentiation factor 2 (Neurod2)

Recombinant Mouse Neurogenic differentiation factor 2 (Neurod2)

  • 中文名称：
    小鼠Neurod2重组蛋白
  • 货号：
    CSB-YP714019MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Neurod2重组蛋白
  • 货号：
    CSB-EP714019MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Neurod2重组蛋白
  • 货号：
    CSB-EP714019MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Neurod2重组蛋白
  • 货号：
    CSB-BP714019MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Neurod2重组蛋白
  • 货号：
    CSB-MP714019MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Neurod2
  • Uniprot No.：
  • 别名：
    Neurod2; Ndrf; Neurogenic differentiation factor 2; NeuroD2; NeuroD-related factor; NDRF
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-383
  • 氨基酸序列
    MLTRLFSEPG LLSDVPKFAS WGDGDDDEPR SDKGDAPPQP PPAPGSGAPG PARAAKPVSL RGGEEIPEPT LAEVKEEGEL GGEEEEEEEE EEGLDEAEGE RPKKRGPKKR KMTKARLERS KLRRQKANAR ERNRMHDLNA ALDNLRKVVP CYSKTQKLSK IETLRLAKNY IWALSEILRS GKRPDLVSYV QTLCKGLSQP TTNLVAGCLQ LNSRNFLTEQ GADGAGRFHG SGGPFAMHPY PYPCSRLAGA QCQAAGGLGG GAAHALRTHG YCAAYETLYA AAGGGGASPD YNSSEYEGPL SPPLCLNGNF SLKQDSSPDH EKSYHYSMHY SALPGSRPTG HGLVFGSSAV RGGVHSENLL SYDMHLHHDR GPMYEELNAF FHN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.
  • 基因功能参考文献：
    1. Using genome-wide, mRNA-based microarray analysis, study found that NeuroD2 is actually one of hundreds of genes whose mRNA levels are suppressed by synaptic activity, in a manner dependent upon N-methyl d-aspartate receptor (NMDAR) activation. Study confirmed this observation both in vitro and in vivo and provide evidence that this happens at the level of transcription and not mRNA stability. PMID: 28524267
    2. Genome-wide target gene analysis of the neurogenic transcription factor NEUROD2 has uncovered a novel gene expression mechanism by which differentiating cortical excitatory neurons fine-tune the extent of store-operated calcium entry. In vivo analysis identified Stim1 (stromal interaction molecule 1) as a primary target of NEUROD2. PMID: 28303257
    3. Our results reveal an important function for NeuroD2 in balancing synaptic neurotransmission and intrinsic excitability and offer insight into how these processes are coordinated during cortical development. PMID: 27146976
    4. Our analysis of in vivo NEUROD2 target genes offers mechanistic insight into signaling pathways that regulate neuronal migration and axon guidance and identifies genes that are likely to be required for proper cortical development. PMID: 26341353
    5. we have identified basic helix-loop-helix (bHLH) transcription factors Neurod2 and Neurod6 as key regulators of fasciculation and targeted axogenesis in the mouse neocortex PMID: 23303943
    6. Over-expression of neuroD2 (ND2) leads to morphological differentiation of neuroblastoma cells and increased expression of synaptic proteins. PMID: 22197973
    7. These experiments identify NeuroD2 as a key transcription factor that regulates the structural and functional differentiation of mossy fiber synapses in vivo. PMID: 22369234
    8. NeuroD2 is expressed in amacrine, ganglion, and bipolar cells of the mature mouse retina. PMID: 21593321
    9. Our study supports a model wherein NeuroD2 induces transcription of neuronal genes and Zfhx1a, which in turn de-represses neuronal differentiation by down-regulating REST, and suppresses competing myogenic fate. PMID: 20346398
    10. expression is probably regulated by immediate-early transcription factors and is thought to be involved in long-lasting neuronal activation. PMID: 11750069
    11. microarray analysis of induction of NDRF and its related bHLH proteins in developing neurons PMID: 16083855
    12. NeuroD2 is essential for amygdala development and genes involved in amygdala function are altered in neuroD2-deficient mice. PMID: 16203979
    13. These data indicate that neuroD2 is expressed throughout the hypothalamic-pituitary-thyroid (HPT) axis and that all levels of the axis are functionally affected by its absence in mice. PMID: 16705180
    14. The expression of NeuroD2 protein requires an appropriate period of about 3 days to elicit neuronal differentiation in embryonic stem cells. PMID: 18996208

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Expressed in layers V and VI of the neocortex at postnatal day 1. Expressed in all layers of the neocortex at postnatal day 4. Strongly
  • 数据库链接：

    KEGG: mmu:18013

    STRING: 10090.ENSMUSP00000041373

    UniGene: Mm.4814