Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Neuroglobin (Ngb)

Recombinant Mouse Neuroglobin (Ngb)

  • 货号：
    CSB-YP015776MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015776MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015776MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015776MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015776MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ngb
  • Uniprot No.：
  • 别名：
    Ngb; Neuroglobin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-151
  • 氨基酸序列
    MERPESELIR QSWRVVSRSP LEHGTVLFAR LFALEPSLLP LFQYNGRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLT SLGRKHRAVG VRLSSFSTVG ESLLYMLEKC LGPDFTPATR TAWSRLYGAV VQAMSRGWDG E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • 基因功能参考文献：
    1. This is the first report demonstrating a similar pattern of Ngb expression during disease progression in the brains of R6/2 mice. PMID: 29101592
    2. NGB proved to act cytoprotectively during MI in the acute setting while this effect was less pronounced in the atherosclerosis model. PMID: 29098407
    3. Study showed that in Neuroglobin (Ngb) knockout mice, distortion-product otoacoustic emissions thresholds were moderately augmented in the range of 5-18kHz. Results suggest that the lack of NgB, results in only marginal deficits in hearing ability. PMID: 29031607
    4. Ngb-H64Q-CCC binds CO about 500 times more strongly than hemoglobin, which could be useful in detecting and eliminating carbon monoxide poisoning PMID: 27928027
    5. Ngb can bind to mitochondrial complex III subunit Cyc1, leading to suppression of oxygen-glucose deprivation -mediated complex III activity. PMID: 26050086
    6. Reduction of Ngb expression in mouse retina after optic nerve injury.Ngb role in neuroregeneration in mice after optic nerve injury. PMID: 28951213
    7. Findings provide evidence for the loss of globin-linked neuroprotection to be linked to Alzheimer's disease angiopathogenesis, while raising the possibility of REST/ NRSF being an upstream regulator. PMID: 28391636
    8. Ngb expression is limited to the subpopulation of type I spiral ganglion cells, those which innervate inner hair cells, while the subpopulation of type II spiral ganglion cells which innervate the outer hair cells do not express Ngb. The cochlear nuclei and superior olivary complex (SOC) also express considerable amounts of Ngb. PMID: 25636685
    9. Ngb functions as a metabolic regulator and enhances cellular anabolism through the inhibition of AMPK signaling. PMID: 25616953
    10. Data show that the neuroglobin (Ngb) protein was downregulated in the retinal ganglion cells after optic nerve injury. PMID: 26427474
    11. Overexpression of neuroglobin reduced sensorimotor deficits following TBI. An endogenous increase in neuroglobin expression occurs during the subacute period. Increasing neuroglobin expression during the acute period after TBI may improve recovery. PMID: 24642455
    12. reflect lesser perturbations produced by acute inhalation of combustion smoke in the Ngb overexpressing mouse, suggesting that Ngb mitigates mitochondrial dysfunction and neurotoxicity and raises the threshold of smoke inhalation-induced brain injury PMID: 24730682
    13. A critical role for neuroglobin is demonstrated via its differential interaction with phosphatase and tensin homolog (PTEN) and Akt during neurite induction. PMID: 23904011
    14. Neuroglobin overexpression resulted in a significant reduction in infarct volume 24 hours after brain ischemia. PMID: 24098534
    15. Ngb overexpression can inhibit oxygen-glucose deprivation-induced mitochondria permeability transition opening in primary cultured mouse cortical PMID: 23639789
    16. CREB may also mediate transcriptional regulation of neuroglobin gene. PMID: 23182882
    17. This study demonistrated that Ngb over-expression reduced traumatic lesion volume. PMID: 22703519
    18. mitochondria distribution of Ngb is clearly associated with and may contribute to Ngb's neuroprotection. PMID: 22659017
    19. VEGF stimulated Ngb expression in a VEGFR2/Flk1 receptor-dependent manner, whereas Ngb overexpression suppressed expression of VEGF. PMID: 22583764
    20. Genetic elimination of neuroglobin evokes an increased behavioural response to light concomitant with increased Per1 gene expression in the suprachiasmatic nucleus. PMID: 22496809
    21. Cerebral regulation of neuroglobin is related to maturational stage; hypoxia-induced neuroglobin up-regulation is modified by the hypoxia-inducible transcription factor (HIF) system. PMID: 22548980
    22. Neuroglobin-deficiency exacerbates Hif1A and c-FOS response, but does not affect neuronal survival during severe hypoxia in vivo PMID: 22164238
    23. NF-kappaB, Egr1 and Sp1 played important roles in the regulation of basal neuroglobin (Ngb) expression via specific interactions with the mouse Ngb promoter PMID: 22239089
    24. Neuroglobin overexpression plays a neuroprotective role against retinal ischemia reperfusion injury due to decreasing of mitochondrial oxidative stress-mediated apoptosis. PMID: 22167093
    25. Neuroglobin is part of the estradiol signaling mechanism that is activated to exert protective effects against hydrogen peroxide-induced neurotoxicity. PMID: 21335947
    26. The study shows that the Fe-heme structure in solution and in the cryo-trapped Ngb-carbonmonoxy single crystal (which cracks at room temperature) are the same. PMID: 20879741
    27. First report of Ngb present in reactive astroglia and in scar-forming astrocytes in response to different pathological conditions. In light of previously reported cyto-protective properties of Ngb, further insight may result in therapeutic ramifications. PMID: 20544857
    28. The anatomical data indicate a role in NO signalling for cytoglobin and involvement in sleep-wake cycling for cytoglobin and neuroglobin PMID: 20331985
    29. The dynamic features of neuroglobin demonstrate rearrangement of the inner space and highly active loop regions in solution. These imply that the conserved residue at the G5 site plays a key role in the physiological function of this unusual protein. PMID: 19820972
    30. After dissociation from the heme iron, the CO ligand visits multiple docking sites identified unambiguously, and identified preferred heme-bound conformation PMID: 19960556
    31. ligand binding and protein dynamics PMID: 12048231
    32. role in supplying oxygen to mammalian retina PMID: 12409290
    33. Neuroglobin mRNA was detected in all brain areas studied and in the peripheral nervous system. Most, but not all, nerve cells were labeled, suggesting differential expression of Ngb. PMID: 12435404
    34. nitric oxide binding properties of neuroglobin PMID: 12480932
    35. neuroglobin immunoreactivity was detected in specific brain regions with varying intensities and in the islet of Langerhans in the pancreas. PMID: 12796507
    36. Neuroglobin is expressed on the protein level in many brain sites including cerebral cortex, thalamus and hypothalamus, nuclei of cranial nerves in the brainstem and cerebellum. PMID: 12850561
    37. crystal structure of murine ferric (met) neuroglobin at 1.5 A reveals interesting features relevant to the ligand binding mechanism PMID: 15162488
    38. Analysis of the neuroglobin genomic sequence identifies sequence motifs with similarity to the neuron-restrictive silencer element, possibly explaining a neuron-specific expression of neuroglobin. PMID: 15193759
    39. oxygen binding in neuroglobin and cytoglobin is allosterically regulated and temperature-dependent PMID: 15299006
    40. The heme relocation is accompanied by a significant decrease of structural disorder, especially of the EF loop, which may be the signal whereby Ngb communicates hypoxic conditions PMID: 15548613
    41. This research showed an age-related decline in Ngb expression in cerebral neocortex, hippocampus, caudate-putamen, and cerebellum. PMID: 15582755
    42. the discovery of Ngb in astrocytes may provide some insight into how oxygen homeostasis is regulated in the brain PMID: 15657899
    43. Neuroglobin is localised in photoreceptor inner segments, the plexiform layers and the ganglion cell layer. PMID: 15663964
    44. Ngb is not regulated by pure hypoxia in vivo in the brain PMID: 16281909
    45. The structural information on the heme pocket of mNgb was revealed by 14N EPR and it is in agreement with previous X-ray diffraction data on neuroglobins. PMID: 16596390
    46. neuroglobin is expressed in distinct regions of the brain, and neuroglobin expression in the brain is not induced in response to chronic hypoxia. PMID: 16899760
    47. Data show that the reduction of ferric neuroglobin by cytochrome b(5) is relatively slow, but the reaction between ferrous neuroglobin and ferric cytochrome c is very rapid. PMID: 16914148
    48. These findings extend prior evidence for cytoprotection by neuroglobin and suggest both direct (parenchymatous) and indirect (vasomotor) protective mechanisms. PMID: 17098866
    49. Ngb expression is correlated with reactive oxygen species scavenging. PMID: 17286577
    50. characterized the heme sliding motion, the topological reorganization of the internal cavities, the dynamics of the distal histidine, and particularly the conformational change of the CD loop, whose flexibility depends ligand binding PMID: 17468165

    显示更多

    收起更多

  • 亚细胞定位：
    Perikaryon. Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Globin family
  • 组织特异性：
    Predominantly expressed in brain.
  • 数据库链接：

    KEGG: mmu:64242

    STRING: 10090.ENSMUSP00000021420

    UniGene: Mm.41395