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  4. Recombinant Mouse Neuronal acetylcholine receptor subunit alpha-4 (Chrna4), partial

Recombinant Mouse Neuronal acetylcholine receptor subunit alpha-4 (Chrna4), partial

  • 货号：
    CSB-YP005390MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005390MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005390MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005390MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005390MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chrna4
  • Uniprot No.：
  • 别名：
    Chrna4; Acra4Neuronal acetylcholine receptor subunit alpha-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
  • 基因功能参考文献：
    1. Data suggest that receptor complex assembly from Chrna4 and Chrnb2 on neuronal cell surface is up-regulated by nicotine; combined effects of increased assembly stoichiometry and preferential trafficking likely drive up-regulation of this receptor complex on neuronal cell surface upon nicotine exposure. (Chrna4 = a4 nicotinic receptor; Chrnb2 = b2 nicotinic receptor) PMID: 29074617
    2. Results suggest that alpha4beta2( *) nAChRs can produce sustained regulation of inhibitory post-synaptic GABA currents, in fast-spiking layer V neurons of the mouse frontal area 2. PMID: 27793780
    3. Mice lacking alpha4 subunits in ventral midbrain consumed significantly more nicotine at the highest offered nicotine concentration compared to control mice. PMID: 28759616
    4. Varenicline treatment may prevent scopolamine-induced memory disturbance by a molecular mechanism that may involve increased alpha4beta2 nAChR availability. Varenicline dose-dependently increased protein expression of both the alpha4 and beta2 subunit in cell cultures and brain tissues, respectively, but had no effect on mRNA expression; lack of change in mRNA expression suggests a still unknown post-transcriptional me... PMID: 27012889
    5. Results demonstrate that high-affinity alpha4beta2 nicotinic acetylcholine receptors are necessary for the effects of acute nicotine on contextual fear extinction. PMID: 26688111
    6. Data show alpha4 subunit role in conferring potentiation by 17beta-estradiol (BEST) and indicate that the C-terminal region is involved in both binding of betaEST and potentiation, while the N-terminal domain seems involved in enhancing its efficacy. PMID: 26684647
    7. The alpha4 nAChR subunit is regulated by protein kinase C epsilon phosphorylation. PMID: 26103136
    8. neuroinflammation is sufficient to provoke the decrease of a7 and a4b2 nAChRs, Ab42 accumulation and memory impairment in mice and a7(1-208) nAChR-specific antibodies can cause inflammation of the brain with symptoms typical for Alzheimer disease. PMID: 25816313
    9. Lynx2 and Ly6g6e have roles in intracellular trafficking and allosteric potentiation of alpha4beta2 nAChRs, respectively PMID: 26276394
    10. cotinine alters the assembly and trafficking of alpha4beta2 nicotinic acetylcholine receptors PMID: 26269589
    11. The study shows that nAChR alpha4 beta2 sensitizes a MAPK-linked toxicity pathway on prolonged exposure to ABETA (1-42). PMID: 26139609
    12. This study has revealed complex effects of Chrna4 deficiency and prenatal nicotine exposure on ventilatory and metabolic interactions and responses to stress. PMID: 25596543
    13. It indicated that Correlation between nAChalpha4-, 5-HT1A- and 5-HT7-containing receptors and latencies on day 16 may point to a probable link in extinction mechanisms. PMID: 24837029
    14. These results question the conclusion that a dominant-negative mechanism would explain the dominance of the mutant L501fsX533 Fz4 allele in the transmission of a form of Familial exudative vitreoretinopathy. PMID: 25086772
    15. Study demonstrates that chronic pre-exposure to nicotine at a dosing regimen that results in maximal alpha4 nAChR upregulation results in significantly elevated oral nicotine self-administration in a two-bottle choice paradigm in mice PMID: 25038610
    16. Lynx1 has a role in shifting alpha4beta2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum PMID: 25193667
    17. these data indicate that a4-nAChR have an impact on motor output. PMID: 23688922
    18. Cerebral cortex alpha4 nAChR subunits increased in rats given nicotine or varenicline, but subunits from rats given saline or saz-A during the phase 2 treatment were not significantly different from all-phase saline controls. PMID: 24422997
    19. a new mechanism of alpha4 nAChR signaling and immune regulation in T cells, possibly accounting for the effect of smoking on the immune system. PMID: 24107512
    20. In this study, alpha4 and alpha7 nicotinic acetylcholinesterase receptors all modulated dopamine-induced abnormal involuntary movements. PMID: 23831952
    21. By stimulating alpha4beta2-nicotinic acetylcholine receptors in the prefrontal cortex, working memory deficits are alleviated in aged mice. PMID: 23352115
    22. alpha4 nAChRs serve as sensors for ethanol-induced oxidant stress in lung fibroblasts. PMID: 23421903
    23. Data indicate that functional alpha4/beta2 nicotinic acetylcholine receptors (alpha4beta2 nAChR) plays a role in proliferation and differentiation fate into a neuronal lineage in neural progenitors of developing neocortex. PMID: 23056257
    24. Stimulation of alpha4-nAChR and alpha7-nAChR by nicotine may lead to a significant increase in the rate of mouse induced pluripotent stem cell proliferation. PMID: 22483693
    25. determined the site specificity for Mor potentiation by studying differences in modulation between alpha3beta2 and alpha4beta2 receptors PMID: 22064677
    26. alpha4* nAChR subunits have a new role in regulating rapid changes in functional responsiveness of the adult somatosensory cortex. PMID: 22279206
    27. In the acute MPTP model of Parkinson's disease, time-courses of altered expression for nAChR alpha7, alpha4 and beta2 subunits closely mirrored the deficits observed in animal motor activity PMID: 21406211
    28. propose that in the P3 superior cervical ganglion of wild type mice, alpha3beta4 (+/-alpha5) represent about 62% of receptors, whereas 17% are alpha3beta2beta4, and 21% are alpha4beta2 (+/-alpha5) receptors PMID: 21485013
    29. Nicotinic acetylcholine receptors containing the alpha4 subunit are critical for the nicotine-induced reduction of acute voluntary ethanol consumption PMID: 21239887
    30. The alpha4 subunit, but not the alpha6 subunit, is necessary for ICSA and nicotine-induced bursting of VTA DAergic neurons, whereas subunits alpha4 and alpha6 together regulate the activity dependence of dopamine transmission in the nucleus accumbens. PMID: 21502501
    31. This study indicated that activation of alpha4* nAChRs is necessary and sufficient for varenicline reduction of alcohol consumption. PMID: 20668200
    32. Compared with Chrna4 T529 littermate controls, the Chrna4 A529 knock-in mice exhibited greater sensitivity to the hypothermic effects of nicotine, reduced oral nicotine consumption and did not develop conditioned place preference to nicotine. PMID: 20061993
    33. a D(2)R-alpha4*-nAChR functional interaction regulates cholinergic interneuron activity PMID: 19720621
    34. Results show that lynx1 promotes the largest of three current amplitudes elicited by ACh through alpha(4)beta(2) nAChRs and that lynx1 enhances receptor desensitization. PMID: 11906696
    35. Results suggest that disruption of balanced cholinergic transmission by constitutive overexpression of acetylcholinesterase is accompanied by variable upregulation of nicotinic receptor subtypes alpha4, alpha 7, and beta 2. PMID: 12059039
    36. A polymorphism in the mouse neuronal alpha4 nicotinic receptor subunit results in an alteration in receptor function. PMID: 12130686
    37. functional nicotinic ACh receptors on stem and progenitor cells of fetal mouse cerebral cortex as early as embryonic day 10 PMID: 12438103
    38. These studies demonstrate that intact alpha 4 nAChR subunits provide significant in vivo protection against the proconvulsant effects of GABA antagonism. PMID: 12623222
    39. Alpha4 nicotinic receptor knock-in mice have increased sensitivity to agonist-induced seizures, straub tail, and hippocampal theta rhythm. PMID: 12684443
    40. This study results indicate that alpha4-containing nAChRs exert a tonic control on striatal basal dopamine release, which is mediated by a heterogeneous population of nAChRs. PMID: 12713636
    41. results suggest that the A/T polymorphism influences the initial sensitivity of the alpha4 nicotinic receptor to ethanol PMID: 12766617
    42. The polymorphism in the mouse alpha4 subunit of Chrna4 is located in a previously unidentified functional domain of the receptor subunit that influences receptor function, including regulation of the affinity of alpha 4 beta 2 receptors. PMID: 12871652
    43. In different strains of mice, polymorphisms in nAChRalpha4 did not clearly correlate with strain-related differences in cell type-specific nAChR expression. PMID: 14681929
    44. data indicate that activation of a4* receptors is sufficient for nicotine-induced reward, tolerance, and sensitization PMID: 15528443
    45. nAChR immunoreactivity is present in genioglossal motoneurons and suggest a role for alpha-4 subunits containing nAChRs in the regulation of upper airway patency. PMID: 15705531
    46. Results suggest that that the up-regulation of alpha4-nicotinic acetylcholine receptor may make cortical neurons more sensitive to the neuroprotective action of donepezil and galanthamine. PMID: 16313899
    47. Hypersensitive alpha4* nicotinic receptors in mice mediate changes in the sleep-wake cycle. PMID: 16339034
    48. An inducible genetic model for degeneration of midbrain dopaminergic neurons in adults, using nAChR subunit 4 transgenic mice, is presented. PMID: 16675851
    49. Together, these results show that immunolabeling is applicable to reliable, quantitative investigations of neuronal nAChRs, and that subunit-null mutants can be appropriate controls for such experiments. PMID: 17072836
    50. discussion of the role of variability in the genes that encode the two major brain-expressed alpha subunits, alpha4 and alpha7, in modulating behavior, physiology and disease risk in both humans and mice [review] PMID: 17981562

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-4/CHRNA4 sub-subfamily
  • 数据库链接：

    KEGG: mmu:11438

    STRING: 10090.ENSMUSP00000066338

    UniGene: Mm.252369