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  4. Recombinant Mouse Nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1)

Recombinant Mouse Nicotinamide mononucleotide adenylyltransferase 1 (Nmnat1)

  • 中文名称：
    小鼠Nmnat1重组蛋白
  • 货号：
    CSB-YP866802MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Nmnat1重组蛋白
  • 货号：
    CSB-EP866802MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Nmnat1重组蛋白
  • 货号：
    CSB-EP866802MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Nmnat1重组蛋白
  • 货号：
    CSB-BP866802MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Nmnat1重组蛋白
  • 货号：
    CSB-MP866802MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nmnat1
  • Uniprot No.：
  • 别名：
    Nmnat1; D4Cole1e; Nmnat; Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1; NMN/NaMN adenylyltransferase 1; EC 2.7.7.1; EC 2.7.7.18; Nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; Nicotinate-nucleotide adenylyltransferase 1; NaMN adenylyltransferase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-285
  • 氨基酸序列
    MDSSKKTEVV LLACGSFNPI TNMHLRLFEL AKDYMHATGK YSVIKGIISP VGDAYKKKGL IPAHHRIIMA ELATKNSHWV EVDTWESLQK EWVETVKVLR YHQEKLATGS CSYPQSSPAL EKPGRKRKWA DQKQDSSPQK PQEPKPTGVP KVKLLCGADL LESFSVPNLW KMEDITQIVA NFGLICITRA GSDAQKFIYE SDVLWRHQSN IHLVNEWITN DISSTKIRRA LRRGQSIRYL VPDLVQEYIE KHELYNTESE GRNAGVTLAP LQRNAAEAKH NHSTL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following mechanical or toxic insults. Delays axonal degeneration after axotomy. Results in a >10-fold increase in intact neurites 72 hours after injury.
  • 基因功能参考文献：
    1. that increasing NMNAT1 levels can slow the progression of symptoms and neuropathological features of tauopathy PMID: 29175372
    2. Using steady-state and flux analysis of NAD(+) metabolites in healthy and injured mouse dorsal root ganglion axons, we find that rather than altering NAD(+) synthesis, NMNAT1 instead blocks the injury-induced, SARM1-dependent NAD(+) consumption that is central to axon degeneration. PMID: 27735788
    3. Mouse-non nuclear-nicotinamide mononucleotide adenylyltransferase type 1 maintains mitochondrial nicotinamide levels and thereby supports mitochondrial unfolded protein response function and mitochondrial proteostasis in injured neurons. PMID: 26913604
    4. NMNAT1 overexpression enhanced AMPK activity in oxygen-glucose deprivation treated cortical neurons PMID: 25841561
    5. An increased level of Nmnat protein in hiw mutants is both required and sufficient to inhibit axonal degeneration. PMID: 23226106
    6. Overexpression of Nmnat1 in the cytoplasm and axons of RGCs robustly protected against both ischemic and glaucomatous loss of RGC axonal integrity, as well as loss of RGC soma. PMID: 23211826
    7. Findings reveal a novel role for NMNAT1 in the morphogenesis of developing cortical neurons, which indicate that the loss of function of NMNAT1 may contribute to different neurodegenerative disorders in central nervous system. PMID: 22088538
    8. Nicotinamide mononucleotide adenylyl transferase 1 protects against acute neurodegeneration in developing CNS by inhibiting excitotoxic-necrotic cell death. PMID: 22058226
    9. NMNAT1 is indispensable for the normal development of the embryo. Decreased NMNAT1 activity in heterozygous null mice does not affect the rate of Wallerian degeneration, suggesting that endogenous NMNAT1 does not have a primary role in axon maintenance. PMID: 21615689
    10. nicotinamide mononucleotide adenylyltransferase (Nmnat) protein transduction into transected axons blocks axonal degeneration PMID: 21071441
    11. Through NAMPT activity, visfatin contributes to vascular inflammation, causing atherothrombotic diseases linked to metabolic disorders. PMID: 19727662
    12. demonstrated that increased Nmnat activity is responsible for the axon-sparing activity of the Wlds protein; also demonstrated that SIRT1 is the downstream effector of increased Nmnat activity that leads to PMID: 15310905
    13. Nmnat1 is significantly weaker than Wld(S) at protecting axons against traumatic or toxic injury in vitro, and has no detectable effect in vivo PMID: 16645633
    14. results suggest that axonal protection by NMNAT expression in neurons is provided by modifying mitochondrial function PMID: 19439605
    15. This study provided the evidence that Nmnat1 protein manifest robust delay in axonal degeneration. PMID: 19458223

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Eukaryotic NMN adenylyltransferase family
  • 数据库链接：

    KEGG: mmu:66454

    STRING: 10090.ENSMUSP00000030845

    UniGene: Mm.76062