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  4. Recombinant Mouse Nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2)

Recombinant Mouse Nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2)

  • 货号：
    CSB-YP804380MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP804380MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP804380MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP804380MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP804380MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nmnat2
  • Uniprot No.：
  • 别名：
    Nmnat2; Kiaa0479; Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2; NMN/NaMN adenylyltransferase 2; EC 2.7.7.1; EC 2.7.7.18; Nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NaMN adenylyltransferase 2; Protein bloated bladder; Blad
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-307
  • 氨基酸序列
    MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ HENTQPIYQN SNVPTKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor. Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons.
  • 基因功能参考文献：
    1. the deregulated expression of Nmnat2 appears to have a great influence on the cellular stress in the spinal cord of wobbler mice. PMID: 29549647
    2. Consistent with Skp1a functioning through regulation of Nmnat2, Skp1a knockdown fails to protect axons from Nmnat2 knockdown. PMID: 27732853
    3. Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829
    4. nicotinamide mononucleotide adenylyltransferase 2-depletion-dependent degeneration of established axons and restricted extension of developing axons are thus both SARM1 dependent PMID: 25818290
    5. Data indicate that thioesterases APT1/APT2 depalmitoylate nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) and zDHHC17 is the strongest candidate palmitoyltransferase for NMNAT2. PMID: 25271157
    6. Complete loss of Nmnat2 leads to a mature but distended bladder in utero and is not compatible with survival. Moderate loss of Nmnat2 has no effect on bladder development, survival, and has only modest effects on bladder function later in life. PMID: 23371862
    7. Together, our results establish Nmnat2 localisation and turnover as a valuable target for modulating axon degeneration in vivo. PMID: 23995269
    8. results suggest an essential role for NMNAT2 during axon growth PMID: 23946398
    9. Nmnat2 activity supports axon survival through a site of action distinct from Nmnat2 transport vesicles. PMID: 23610559
    10. Nmnat2 is involved in axon development or survival in a mammal. PMID: 23082226
    11. Decreased endogenous NMNAT2 function caused by reduced CREB signaling during pathological insults may be one of underlying mechanisms for neuronal death in tauopathies PMID: 22027994
    12. Nmnat2 is a neuronal protein peripherally attached to membranes via palmitoylation and suggest that Nmnat2 is transported to synaptic terminals via an endosomal pathway. PMID: 20943658
    13. The brain-specific Nmnat2 delays injury-induced axon degeneration dependent on its NAD synthesis activity. These findings provide new clues to further study the molecular mechanisms of axon degeneration and the related neurodegenerative diseases. PMID: 19778564

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  • 亚细胞定位：
    Golgi apparatus membrane; Lipid-anchor. Cytoplasmic vesicle membrane; Lipid-anchor. Cytoplasm. Cell projection, axon.
  • 蛋白家族：
    Eukaryotic NMN adenylyltransferase family
  • 组织特异性：
    Expressed predominantly in the brain and nervous system.
  • 数据库链接：

    KEGG: mmu:226518

    STRING: 10090.ENSMUSP00000041110

    UniGene: Mm.40548