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  4. Recombinant Mouse Norrin (Ndp)

Recombinant Mouse Norrin (Ndp)

  • 中文名称：
    小鼠Ndp重组蛋白
  • 货号：
    CSB-YP015609MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ndp重组蛋白
  • 货号：
    CSB-EP015609MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ndp重组蛋白
  • 货号：
    CSB-EP015609MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ndp重组蛋白
  • 货号：
    CSB-BP015609MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ndp重组蛋白
  • 货号：
    CSB-MP015609MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ndp
  • Uniprot No.：
  • 别名：
    Ndp; NdphNorrin; Norrie disease protein homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-131
  • 氨基酸序列
    KTDSSF LMDSQRCMRH HYVDSISHPL YKCSSKMVLL ARCEGHCSQA SRSEPLVSFS TVLKQPFRSS CHCCRPQTSK LKALRLRCSG GMRLTATYRY ILSCHCEECS S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction.
  • 基因功能参考文献：
    1. NORRIN presented to FZD4 further increases cardiomyocyte output via proliferation through the canonical WNT pathway. PMID: 29249665
    2. NDP is a potent trigger of FZD4 ubiquitination and induces internalization of the NDP receptor complex into the endo-lysosomal compartment. Inhibition of ubiquitinated cargo transport strongly impairs NDP/FZD4 signalling in vitro and recapitulates CNS angiogenesis and blood-CNS-barrier defects caused by impaired vascular beta-catenin signalling in mice. PMID: 28675177
    3. results provide functional and biochemical dissection of Fzd4 in Norrin signaling. PMID: 30104375
    4. The endogenously expressed Lgr4 may act as an antagonist molecule that helps to fine-tune the R-spondin/norrin-mediated Lgr4-Wnt signaling during gonadal development. PMID: 25188337
    5. Multi-functional norrin is a ligand for the LGR4 receptor. PMID: 23444378
    6. In this study we demonstrate, for the first time, that Norrin protein is expressed along the retinal blood vessels. PMID: 23206555
    7. Taken together, we have uncovered a cell autonomous function for Ndp in retinal progenitor proliferation that is independent of its function in the retinal vasculature, which could explain the neural defects associated with Norrie disease PMID: 23201751
    8. We conclude that constitutive overexpression of Norrin protects photoreceptors from light damage, an effect that is mediated by Wnt/beta-catenin and EDN2 signaling and involves neurotrophic activities of BDNF. PMID: 23009755
    9. The data reveal both cell-autonomous and cell-nonautonomous effects, and they imply a central role for Norrin/Fz4 signaling in central nervous system vascular development and in the maintenance of the blood brain barrier/blood retina barrier state. PMID: 23217714
    10. Results suggest that the delayed outgrowth of the SRVP and decreased angiogenic sprouting in Norrin knockout (Ndp(y/-)) mice are direct effects of the reduced proliferation of endothelial cells from the superficial retinal vascular plexus (SRVP). PMID: 22394677
    11. Norrin has a neuroprotective role for retinal neurons independent from its role on the growth of retinal capillaries. PMID: 22183393
    12. These observations suggest the possibility that Norrin may have developmental and/or homeostatic functions beyond the retina and cochlea. PMID: 21055480
    13. Norrin has pronounced neuroprotective properties on retinal neurons. The effects of Norrin involve activation of Wnt/beta-catenin signaling and subsequent induction of neurotrophic growth factors in Muller cells. PMID: 20427659
    14. a Norrin-Fz4 signaling system plays a central role in vascular development in the eye and ear, and ligands unrelated to Wnts can act through Fz receptors PMID: 15035989
    15. Transgenic expression of ectopic norrin under control of a lens-specific promoter restores the formation of a normal retinal vascular network in Ndp(y/-) mutant mice. PMID: 15716406
    16. These data provide strong evidence for Norrin playing an important role in female reproductive tissues. PMID: 16035034
    17. This provides the first description of a brain phenotype in Ndph knockout mice, which will help to elucidate the role of Norrin in the brain. PMID: 18547247
    18. These data provide molecular evidence for a role of Norrin in the development of the retinal vasculature. PMID: 18978344
    19. Data indicate that Norrin multimers and TSPAN12 cooperatively promote multimerization of FZD4 and its associated proteins to elicit physiological levels of signaling. PMID: 19837033

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in the outer nuclear, inner nuclear and ganglion cell layers of the retina.
  • 数据库链接：

    KEGG: mmu:17986

    STRING: 10090.ENSMUSP00000046692

    UniGene: Mm.5014