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  4. Recombinant Mouse Nuclear distribution protein nudE-like 1 (Ndel1)

Recombinant Mouse Nuclear distribution protein nudE-like 1 (Ndel1)

  • 中文名称：
    小鼠Ndel1重组蛋白
  • 货号：
    CSB-YP861696MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ndel1重组蛋白
  • 货号：
    CSB-EP861696MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ndel1重组蛋白
  • 货号：
    CSB-EP861696MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ndel1重组蛋白
  • 货号：
    CSB-BP861696MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ndel1重组蛋白
  • 货号：
    CSB-MP861696MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ndel1
  • Uniprot No.：
  • 别名：
    Ndel1; Nudel; Nuclear distribution protein nudE-like 1; Protein mNudE-like; Protein Nudel; mNudE-L
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MDGEDIPDFS SLKEETAYWK ELSLKYKQSF QEARDELVEF QEGSRELEAE LEAQLVQAEQ RNRDLQADNQ RLKYEVEALK EKLEHQYAQS YKQVSVLEDD LSQTRAIKEQ LHKYVRELEQ ANDDLERAKR ATIVSLEDFE QRLNQAIERN AFLESELDEK ESLLVSVQRL KDEARDLRQE LAVRERQQEV TRKSAPSSPT LDCEKMDSAV QASLSLPATP VGKGTENSFP SPKAIPNGFG TSPLTPSARI SALNIVGDLL RKVGALESKL AACRNFAKDQ ASRKSYVPGS VNCGVMNSNG PECPRSGRAT FFHKGAVNGF DPAPPPPGLG SSRPSSAPGM LPLSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts.
  • 基因功能参考文献：
    1. results highlight S-Nitrosylation as a key activity-dependent mechanism underlying neonatal brain maturation and suggest that reduction of S-Nitrosylation of NDEL1 acts as a pathological factor mediating neurodevelopmental abnormalities caused by maternal alcohol exposure PMID: 27371763
    2. This study showed that Ndel1 Maintain Postnatal CA1 Hippocampus Integrity in mice. PMID: 27307241
    3. Data indicate that NDEL1 is inducibly expressed in the adult nervous system, and signaling via the CREB/CRE transcriptional pathway is likely involved. Role of NDEL1 in neuronal migration and neurite outgrowth during development raises the interesting prospect that inducible NDEL1 in the mature nervous system could contribute to the well-characterized structural and functional plasticity resulting from repetitive seizures PMID: 27298008
    4. NDEL1 facilitates the reversal of somatodendritic traansport in the proximal axon. PMID: 26844830
    5. study provided comprehensive documentation of the expression patterns of Nde1 and Ndel1 in cultured cells as well as in mouse and human brains, and also highlighting that dosage effects of these two proteins might contribute to some cases of mental disorder PMID: 24785679
    6. Results suggest that NDEL1 might interact with DISC1 to activate ERK signaling and function as a potential protective factor during the spontaneous seizure period after pilocarpine-induced status epilepticus PMID: 24680936
    7. Results show that LIS1 acts via the LIS1-NDEL1-dynein complex to regulate astral microtubule MT plus-ends dynamics and establish proper contacts of MTs with the cell cortex to ensure precise cell division. PMID: 24030547
    8. Nuclear distribution element-like 1 (Ndel1 or Nudel) was firstly described as a regulator of the cytoskeleton in microtubule and intermediate filament dynamics and microtubule-based transport. PMID: 21948775
    9. Expressing a mutant Ndel1 lacking key phosphorylation sites shuts down organelle transport completely in axons. PMID: 22114287
    10. palmitoylated Ndel1 reduced cytoplasmic dynein activity as judged by Golgi distribution, VSVG and short microtubule trafficking, transport of endogenous Ndel1 and LIS1 from neurite tips to the cell body and neuronal migration PMID: 19927128
    11. Lis1 and Ndel1 are essential for normal cortical neuronal migration, neurite outgrowth, and function of the microtubule organizing center in a dose-dependent manner. PMID: 20007476
    12. The predicted peptide product resulting from the Scottish translocation removes the interaction domain for Nudel. Nudel is able to act as a bridge between DISC1 and Lis1 to allow formation of a trimolecular complex. PMID: 14962739
    13. NUDEL is important for NF assembly, transport and neuronal integrity. NUDEL facilitates the polymerization of NFs through a direct interaction with the NF light subunit (NF-L). PMID: 15208636
    14. Ndel1 deficiency results in neuronal migration defects and early embryonic lethality PMID: 16107726
    15. Aurora-A-mediated phosphorylation of NDEL1 is essential for centrosomal separation and centrosomal maturation and for mitotic entry PMID: 17060449
    16. Nudel facilitates cell migration by sequestering Cdc42GAP at the leading edge to stabilize active Cdc42 in response to extracellular stimuli. PMID: 18331715
    17. Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration. PMID: 18431495
    18. Results report that reduction of Lis1 and Ndel1 in a mitotic cell line impairs prophase nuclear envelope invagination. PMID: 18809722
    19. These findings demonstrate a surprising role of aPKC-Aurora A-NDEL1 pathway in microtubule remodelling during neurite extension. PMID: 19668197

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    NudE family
  • 组织特异性：
    Expressed in brain, liver, lung and testis (at protein level). Expressed in brain, epididymis, eye, heart, kidney, large intestine, liver, ovary, pancreas, prostate, skeletal muscle, smooth muscle, spleen, submaxillary gland, testis, thymus and thyroid. W
  • 数据库链接：

    KEGG: mmu:83431

    STRING: 10090.ENSMUSP00000018880

    UniGene: Mm.31979