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  4. Recombinant Mouse Nuclear transition protein 2 (Tnp2)

Recombinant Mouse Nuclear transition protein 2 (Tnp2)

  • 货号：
    CSB-YP024019MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024019MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024019MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024019MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024019MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tnp2
  • Uniprot No.：
  • 别名：
    Tnp2; Nuclear transition protein 2; TP-2; TP2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-117
  • 氨基酸序列
    MDTKMQSLPT THPHPHSSSR PQSHTSNQCN QCTCSHHCRS CSQAGHAGSS SSPSPGPPMK HPKPSVHSRH SPARPSHRGS CPKNRKTFEG KVSKRKAVRR RKRTHRAKRR TSGRRYK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals. In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction. The histone H2AB1-H2BC1/TH2B dimer is required for loading of TNP2 onto chromatin.
  • 基因功能参考文献：
    1. juvenile spermatids from Ip6k1(-/-) mice display premature expression of the transition protein TNP2 and the protamine PRM2 due to translational derepression. PMID: 28743739
    2. The association of Tp2 expression via binding to the conserved RNA binding motifs of GRTH protein and the basis for understanding GRTH in the regulation of the genes essential for germ cell elongation and completion of spermatogenesis is revealed. PMID: 25910401
    3. Testis-specific miRNA-469 up-regulated in gonadotropin-regulated testicular RNA helicase (GRTH/DDX25)-null mice silences transition protein 2 and protamine 2 messages at sites within coding region: implications of its role in germ cell development. PMID: 22086916
    4. Cannabinoid receptor 1 influences chromatin remodeling in mouse spermatids by affecting content of transition protein 2 mRNA. PMID: 20810562
    5. The elevated levels of the remaining TP in Tnp-mutant mice appeared to be a consequence of the prolonged nucleus retention, rather than increased synthesis PMID: 15163613
    6. Epididymal sperm from Tnp2 null genotypes demonstrated a general decrease in normal morphology, motility, chromatin condensation, and degree of protamine 2 processing with decreasing levels of transition proteins in mutant sperm. PMID: 15189834
    7. Specific microRNAs, such as Mirn122a, could be involved in the posttranscriptional regulation of mRNAs such as Tnp2 in the mammalian testis. PMID: 15901636
    8. These results show clearly that a strict temporal and stage-specific Tnp2 translation is necessary for the correct differentiation of round spermatids into mature spermatozoa and for male fertility. PMID: 16967499
    9. Data show that Parp2 interacts significantly with both the transition protein TP2 and the transition chaperone HSPA2, whereas Parp1 binds weakly to HSPA2. PMID: 19607827

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Nuclear transition protein 2 family
  • 数据库链接：

    KEGG: mmu:21959

    STRING: 10090.ENSMUSP00000053078

    UniGene: Mm.206798