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  4. Recombinant Mouse Optineurin (Optn)

Recombinant Mouse Optineurin (Optn)

  • 货号：
    CSB-YP016363MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016363MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016363MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016363MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016363MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Optn; Optineurin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-584
  • 氨基酸序列
    MSHQPLSCLT EKGDSPCETP GNGPSNMVHP SLDTFTPEEL LQQMKELLVE NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERLLFEMQS KEVKERLKAL THENERLKEE LGKFKEKSEK PLEDLTGGYR YPRALEEEVE KLKTQVEQEV EHLKIQVMRL RAEKADLLGI VSELQLKLNS GGSSEDSFVE IRMTEGETEG AMKEMKNCPT PTRTDPISLS NCTEDARSCA EFEELTVSQL LLCLREGNQK VERLEVALRE AKERISDFEK KANGHSSTEK QTARRADREK EDKGQESVGS EVETLSIQVT SLFKELQEAH TKLSEAELMK KRLQEKCQAL ERKNSATPSE LNEKQELVYS NKKLELQVES MRSEIKMEQA KTEEEKSRLA TLQATHNKLL QEHNKALKTI EELTKQQAEK VDKMLLQELS EKLELAEQAL ASKQLQMDEM KQTLAKQEED LETMAVLRAQ MEVYCSDFHA ERAAREKIHE EKEQLALQLA ILLKENNDIE EGGSRQSLME MQCRHGARTS DSDQQTYLFQ RGAEDRSWQH GQQPRSIPIH SCPKCGEVLP DIDTLQIHVM DCII
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.
  • 基因功能参考文献：
    1. findings suggest that changes in their accumulation, determined via quantitative comparison of the OPTN foci and IBs in the cells, are involved in pathological features of ALS PMID: 29684153
    2. These results suggest that the IRAK1-binding protein OPTN negatively regulates IL-1beta/LPS-induced NF-kappaB activation by preventing polyubiquitination of TRAF6. PMID: 28882891
    3. This study therefore provides novel information regarding the role of Optn during T-cell activation, suggesting the possible importance of Optn during inflammation and/or autoimmune diseases. PMID: 28192730
    4. Report establishes optineurin as a positive regulator TBK1 via a bipartite interaction between these molecules. PMID: 27086836
    5. OPTN-deficient mice were more susceptible to infection with Salmonella, confirming in vivo the importance of OPTN in bacterial clearance. PMID: 26677802
    6. Optineurin knockdown enhances osteoclast differentiation. Optineurin inhibits osteoclast formation by modulating NF-kappaB and IFN-beta signaling. PMID: 26527009
    7. Mutation in Optineurin gene is associated with Glaucoma. PMID: 26376340
    8. Disruption in optineurin and myosin VI-mediated cellular trafficking is associated with amyotrophic lateral sclerosis. PMID: 25859013
    9. OPTN plays a role in acute inflammation and neutrophil recruitment, potentially via defective macrophage proinflammatory cytokine secretion. PMID: 26044960
    10. Its mutation is associated with open-angle glaucoma. PMID: 25818176
    11. that ubiquitin (Ub)-binding domain mutants compromise the maturation of autophagosomes, which in turn interfered with optineurin-mediated autophagy and clearance of inclusion bodies PMID: 25484089
    12. results indicated that optineurin trimers may be the basic unit of these oligomers. The oligomeric state can be affected by many factors that induce covalent bonds, such as H2O2 or E50K PMID: 24983867
    13. Loss of optineurin in vivo results in elevated cell death and alters axonal trafficking dynamics PMID: 25329564
    14. HACE1-OPTN axis synergistically suppresses growth and tumorigenicity of lung cancer cells. PMID: 25026213
    15. Mutants in which the entire Ub-binding C-terminal region is deleted showed that optineurin is dispensable for NF-kappaB activation but necessary for optimal IRF3 activation in immune cells. PMID: 24244017
    16. We found that OPTN knockdown induced neuronal cell death via NF-kappaB activation PMID: 23721573
    17. We suggest that OPTN abundance is an important cellular factor in considering the cell type-specific vulnerability of striatal neurons in Huntington's disease PMID: 22155493
    18. OPTN binds to polyubiquitylated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFNbeta. PMID: 21862579
    19. Transgenic optineurin is involved in the pathogenesis of sporadic amyotropic lateral sclerosis and non-SOD1 familial ALS, thus supporting the hypothesis that these forms of ALS share a pathway that is distinct from that of SOD1-linked ALS. PMID: 21825243
    20. Introduction of the E50K mutation disrupts the interaction between Optn and Rab8 GTPase, a protein involved in the regulation of vesicle transport from Golgi to plasma membrane. PMID: 20388642
    21. Optn colocalizes with vesicular structures near the nucleus and is expressed in anterior segment, retina, and optic nerve blood vessels. PMID: 15607428
    22. mutated form of optineurin, E50K, identified in normal tension loses its ability to translocate to the nucleus and when overexpressed compromises the mitochondrial membrane integrity resulting in cells that are less fit to survive under stress conditions PMID: 16569640
    23. Optn expression is triggered during early stages of eye development. Expression of Optn gene in ocular tissues during mouse embryogenesis correlates with presence and distribution of optineurin protein previously reported in adult tissues. PMID: 17893645
    24. TNF-alpha, an inflammatory cytokine, disrupts the localization of polycystin-2 to the plasma membrane and primary cilia through a scaffold protein, FIP2, which is induced by TNF-alpha PMID: 18552856

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  • 亚细胞定位：
    Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle. Recycling endosome.
  • 组织特异性：
    In eye, it is expressed in anterior segment, retina, and optic nerve blood vessels (at protein level). Highly expressed in adult liver, heart and testis.
  • 数据库链接：

    KEGG: mmu:71648

    STRING: 10090.ENSMUSP00000027986

    UniGene: Mm.116942