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  4. Recombinant Mouse Pancreas/duodenum homeobox protein 1 (Pdx1)

Recombinant Mouse Pancreas/duodenum homeobox protein 1 (Pdx1)

  • 中文名称：
    小鼠Pdx1重组蛋白
  • 货号：
    CSB-YP017746MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pdx1重组蛋白
  • 货号：
    CSB-EP017746MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pdx1重组蛋白
  • 货号：
    CSB-EP017746MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pdx1重组蛋白
  • 货号：
    CSB-BP017746MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pdx1重组蛋白
  • 货号：
    CSB-MP017746MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdx1
  • Uniprot No.：
  • 别名：
    Pdx1; Ipf1Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; IPF-1; Islet/duodenum homeobox 1; IDX-1; Somatostatin-transactivating factor 1; STF-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MNSEEQYYAA TQLYKDPCAF QRGPVPEFSA NPPACLYMGR QPPPPPPPQF TSSLGSLEQG SPPDISPYEV PPLASDDPAG AHLHHHLPAQ LGLAHPPPGP FPNGTEPGGL EEPNRVQLPF PWMKSTKAHA WKGQWAGGAY TAEPEENKRT RTAYTRAQLL ELEKEFLFNK YISRPRRVEL AVMLNLTERH IKIWFQNRRM KWKKEEDKKR SSGTPSGGGG GEEPEQDCAV TSGEELLAVP PLPPPGGAVP PGVPAAVREG LLPSGLSVSP QPSSIAPLRP QEPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
  • 基因功能参考文献：
    1. Maturation of pancreatic beta-cells requires Pdx1 function in cooperation with Foxa2. PMID: 28580283
    2. The dynamic undifferentiated cell states within the male germline expressing PDX1 have been found and characterized. PMID: 30026551
    3. results indicated that conditioned medium, just as critical components of the stem cell niche associated with other factors, such as Pdx1, had high potential to differentiate mESCs into IPCs PMID: 29637395
    4. the present study demonstrated that Pdx1+/-/Alzheimer mice exhibit enhanced cognitive decline, amyloid-beta plaque deposition, tau hyperphosphorylation, the loss of synaptic spine protein, and activation of microglia and astrocytes. PMID: 27406855
    5. This study showed that altered Pdx1 mRNA and protein levels in weaned male mutant beta-cells were tightly linked with hyperglycemia, decreased beta-cell proliferation, reduced beta-cell area, and altered expression of Pdx1-bound genes that are important in beta-cell replication, endoplasmic reticulum function, and mitochondrial activity. PMID: 28705881
    6. These results suggest that Oc1 and Pdx1 cooperate prior to their divergence, in pancreatic progenitors, to allow for proper differentiation and functional maturation of beta cells. PMID: 27292642
    7. Data suggest that hepatocytes can be reprogrammed into insulin-producing cells in vivo by transfection of neurogenin-3, Pdx1, and MafA genes using non-viral hydrodynamics injection; this procedure was used in treatment of streptozotocin diabetes; fasting blood glucose was reduced to normal. (Pdx1 = pancreatic and duodenal homeobox 1; MafA = v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A) PMID: 28100871
    8. Creating the Pdx1(DeltaAREAII) state after cells entered an insulin-expressing stage led to immature and dysfunctional islet beta cells carrying abnormal chromatin marking in vital beta-cell-associated genes. Therefore, trans-regulatory integration through Area II mediates a surprisingly extensive range of progenitor and beta-cell-specific Pdx1 functions. PMID: 27993987
    9. The use of a GFP reporter knocked into the endogenous Pdx1 promoter allowed us to monitor pancreatic induction based on the expression of Pdx1, a pancreatic master transcription factor, and to isolate a pure Pdx1-GFP(+) population for downstream applications. PMID: 27181524
    10. Upon neoplastic transformation, the role of PDX1 changes from tumor-suppressive to oncogenic PMID: 28087712
    11. PDX1 role in beta-cell function and diabetes PMID: 28017717
    12. Diabetes Caused by Elastase-Cre-Mediated Pdx1 Inactivation PMID: 26887806
    13. These results suggest that Bax mediates beta-cell apoptosis in Pdx1-deficient diabetes. PMID: 27137932
    14. the role of Epidermal Growth Factor (EGF) and Poly-L-Ornithine (PLO) and PDX1 on human amnion epithelial cell differentiation process, is reported. PMID: 27068127
    15. Ets-1 links glucotoxicity to pancreatic beta cell dysfunction through inhibiting PDX-1 expression in type 2 diabetes. PMID: 26564177
    16. Pdx1+Sox9 co-occupy regulatory sequences of pancreatic and intestinal genes. Pdx1+Sox9 are necessary and sufficient to repress the intestinal fate choice. PMID: 26440894
    17. The paper identifies two conserved Pdx1 binding sites in the epithelial cadherin (E-cad, Cdh1) promoter, and shows that Pdx1 directly binds and activates E-cad transcription. PMID: 26657766
    18. Introduction of the PDX1 gene makes cells acquire a functional insulin secretory capacity PMID: 25817342
    19. physiological and pathophysiological conditions dynamically regulated Pdx1 binding to these Swi/Snf complexes in vivo PMID: 25801033
    20. The novel Pdx1-Clec16a-Nrdp1 pathway this study describes provides a genetic basis for the pathogenesis of mitochondrial dysfunction in multiple forms of diabetes. PMID: 26085571
    21. Pdx1 protein levels are maintained in the setting of the UPR. PMID: 25251389
    22. Data indicate that the ability of transcription factor Pdx1 to regulate genes in beta cells is partially dependent upon its methylation by methyltransferase Set7/9. PMID: 25713082
    23. Results indicate sarco-endoplasmic reticulum calcium ATPase 2b (SERCA2b) as a transcriptional target of Pancreatic and duodenal homeobox protein 1 (Pdx-1). PMID: 25271154
    24. Pdx1, USF1 and USF2 co-ordinately regulate Alx3 gene expression in pancreatic beta-cells. PMID: 25040025
    25. The findings indicate that Pdx1 acts as a master regulator of beta cell fate by simultaneously activating genes essential for beta cell identity and repressing those associated with alpha cell identity. PMID: 24506867
    26. Pdx1 gene expression was knocked down in mouse MIN6 insulinoma cells resulting in apoptotic cell death that was found to be associated with increased expression of BH3-only molecules Bim and Puma. PMID: 24658302
    27. Data indicate that CK2 is a co-factor of PDX-1 in response to glucose in pancreatic beta-cells. PMID: 24126110
    28. three transcription factors, PDX1, NGN3 and MAFA, are very important in pancreatic development. Overexpression of these three factors can reprogram both pancreatic exocrine and SOX9-positive cells of the liver into cells resembling pancreatic beta cells PMID: 24312421
    29. Data indicate that mice in which Pdx1 was chronically repressed developed hamartomas in the gastro-duodenal domain. PMID: 23873371
    30. The expression of exogenous Pdx-1 is sufficient to induce pancreatic differentiation in vitro. PMID: 23031216
    31. Hes-1 downregulation allows PDX-1 to act as a major determinant of cholangiocyte proliferation in response to cholestatic injury. PMID: 23207146
    32. These results suggest that a secreted luciferase reporter system in vivo enables not only the monitoring of promoter activity but also a quantitative and minimally invasive assessment of physiological and pathological changes in small cell masses. PMID: 24029239
    33. Findings suggest that Pdx1 is not necessary for the postnatal formation of beta-cells but is essential for their full maturation to glucose-responsive beta-cells. PMID: 23775765
    34. Pdx-1 activates islet alpha- and beta-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. PMID: 23938296
    35. PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells. PMID: 23853095
    36. Mouse mesenchymal stem cells differentiate into effective glucose-responsive insulin producing cells through recombinant lentiviral transduction of the pdx-1 gene in vitro. PMID: 23831626
    37. Characterization of an apparently novel beta-cell line-enriched 80-88 kDa transcriptional activator of the MafA and Pdx1 genes. PMID: 23269676
    38. PDX-1 is a therapeutic target for both hormonal symptoms and tumor volume in models of pancreatic cancer, insulinoma and islet neoplasia PMID: 22905092
    39. GATA4 and GATA6 transcription factors bind to 2 conserved GATA sites in the conserved area III of Pdx1 which are required for promoter activity. Pdx1 is a direct target of GATA factors. PMID: 23006330
    40. SSTR5 is a negative regulator for PDX-1 expression and SSTR5 may mediate the inhibitory effects of somatostatin and its analogs on insulin expression/secretion and cell proliferation via down-regulating PDX-1 PMID: 22669743
    41. Pdx1 mediates lipid metabolism gene networks in intestinal cells and that maternal expression is essential for perinatal growth in mice. PMID: 22771330
    42. Results indicate that Pdx1 and MafA drive DE progenitors towards insulin-producing cells. PMID: 22563503
    43. At the chromatin level, histone tail modifications of the Pdx1, Ins1 (insulin 1) and Ins2 (insulin 2) gene promoters are shifted in a direction associated with gene activity PMID: 22150363
    44. pdx1 may regulate lipid metabolism in the duodenum and iron transport across the duodenal intestinal mucosa. PMID: 22135308
    45. Forced expression of PLAG1 under the control of the Pdx1 or Ngn3 promoter in murine pancreas induces different degrees of endocrine hormone imbalances. PMID: 21294959
    46. Findings show that the utility of manipulating Pdx1 and Ngn3 expression in a stage-specific manner as an important new strategy for the efficient generation of functionally immature insulin-producing beta-islet cells from ES cells. PMID: 21931641
    47. these results suggest that ATF3 inhibits PDX-1-mediated transactivation through the inhibition of p300-stimulated coactivation, which may lead to beta-cell dysfunction by ER stress. PMID: 21986529
    48. Findings show that Pdx1 can work single-handedly as a potent context-dependent autonomous reprogramming agent, and suggest a postnatal differentiation evaluation stage involved in normal endocrine maturation. PMID: 21852533
    49. these results demonstrate that ATF3 represses PDX-1 expression via binding to an ATF3-responsive element in its promoter, which plays an important role in suppression of pancreatic beta-cells function. PMID: 21821004
    50. 14-3-3epsilon interacts directly with PDX-1 to regulate its cellular distribution in pancreatic beta cells. PMID: 21287435

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    Antp homeobox family, IPF1/XlHbox-8 subfamily
  • 组织特异性：
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • 数据库链接：

    KEGG: mmu:18609

    STRING: 10090.ENSMUSP00000082729

    UniGene: Mm.389714