Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Paxillin (Pxn)

Recombinant Mouse Paxillin (Pxn)

  • 货号：
    CSB-YP019112MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019112MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019112MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019112MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019112MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    PxnPaxillin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-591
  • 氨基酸序列
    MDDLDALLAD LESTTSHISK RPVFLSEEPP YSYPTGNHTY QEIAVPPPVP PPPSSEALNG TVLDPLDQWQ PSGSRYAHQQ PPSPLPVYSS SAKNSSASNT QDGVGSLCSR AGEEEHVYSF PNKQKSAEPS PTVMSSSLGS NLSELDRLLL ELNAVQHSPP GFPADEAESS PPLPGALSPL YGIPENNTPL GGKAGPLVKE KPKRNGGRGL EDVRPSVESL LDELESSVPS PVPAITVNQG EMSSPQRVTS SQQQTRISAS SATRELDELM ASLSDFKMQG LEQRVDGERP WAAGWPPSSR QSSPEGQDEG GFMAQGKTGS SSPPGGLSKP GSQLDSMLGS LQSDLNKLGV ATVAKGVCGA CKKPIAGQVV TAMGKTWHPE HFVCTHCQEE IGSRNFFERD GQPYCEKDYH SLFSPRCYYC NGPILDKVVT ALDRTWHPEH FFCAQCGAFF GPEGFHEKDG KAYCRKDYFD MFAPKCGGCA RAILENYISA LNTLWHPECF VCRECFTPFV NGSFFEHDGQ PYCEVHYHER RGSLCSGCQK PITGRCITAM AKKFHPEHFV CAFCLKQLNK GTFKEQNDKP YCQSCFVKLF C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 基因功能参考文献：
    1. the focal adhesion component paxillin is a cytoplasmic substrate of Ccnd1.Cdk4. PMID: 27181366
    2. Kindlin-2 is a key protein that couples cell adhesion by activating integrins and the induction of membrane protrusions by activating Rac1 and supplying Rac1 with the Arp2/3 complex. PMID: 28912124
    3. paxillin and FAK function cell-autonomously to control migrating neuron morphology and speed during cortical development. PMID: 28935710
    4. paxillin interacts with processed LC3 through a conserved LIR motif in the amino-terminal end of paxillin and that this interaction is regulated by oncogenic SRC activity. PMID: 27184837
    5. Data show that actomyosin cytoskeletal contractility is required for paxillin localization to developing somite boundaries. PMID: 28315297
    6. paxillin and Hic-5 have both redundant and distinctive functions in invadosome formation. PMID: 27269065
    7. Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation PMID: 26881253
    8. Lithium therapy abrogated GSK3beta overactivity, blunted paxillin hyperphosphorylation, and reinstated actin cytoskeleton integrity in glomeruli associated with an early attenuation of podocyte foot process effacement. PMID: 25239564
    9. Suggest paxillin up-regulation and phosphorylation as an important mechanism of vascular remodelling underlying pulmonary hypertension. PMID: 25231004
    10. LPS-induced paxillin phosphorylation at Y31 and Y118 was mediated by c-Abl tyrosine kinase PMID: 25795725
    11. Study provides evidence that phosphorylation of PXN is required for cisplatin resistance in lung cancer cells. PMID: 24096476
    12. FAK-paxillin interactions are indispensable for cell adhesion, migration and invasion processes. PMID: 24642576
    13. paxillin and zyxin are LIM-domain adaptor proteins that are recruited to stress fiber strain sites PMID: 23990882
    14. SLK phosphorylates the adhesion adapter protein paxillin on serine 250. Serine 250 phosphorylation is required for paxillin redistribution and cell motility. PMID: 23128389
    15. Paxillin is critical for integrating physical cues from the ECM with chemical motility signals by spatially constraining where cells form motile processes, and thereby regulates directional migration. PMID: 23076140
    16. Paxillin is a new regulator protein of pulmonary arterial smooth muscle cell growth. PMID: 22959909
    17. The paxillin is the substrate of JNK in Schwann cell migration but JNK targets other than paxillin may be involved in the signaling pathways. PMID: 22750292
    18. These data identify discrete roles for paxillin and Hic-5 in Rac1 and RhoA-dependent cell adhesion formation and maturation; processes essential for productive cell migration. PMID: 22629471
    19. Paxillin integrates physical and chemical motility signals by spatially constraining where cells will form motile processes, and thereby regulates directional migration. PMID: 22194823
    20. Although Pxn expression is visible throughout the cochlear duct, at pre- and early post-natal stages it is always most marked in the pillar cells. PMID: 22446089
    21. Paxillin and N-WASP are involved in the reorganization of actin cytoskeleton driving motility of LPS-stimulated cells. PMID: 21898535
    22. paxillin is recruited to sites of integrin engagement and may contribute to microtubule organizing center reorientation required for directional degranulation. PMID: 22043013
    23. Paxillin was found to be localized to some, but not all ridges in the neutrophil footprint. PMID: 21418380
    24. overexpression of flightless i inhibits paxillin activity and reduces adhesion signaling by modulating the activity of the Rho family GTPases PMID: 21430700
    25. Paxillin contributes to macrophage motility in a pathway that is separate from that of focal adhesion kinase. PMID: 21084629
    26. The phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. PMID: 20308429
    27. leupaxin functions as a paxillin counterpart that potently suppresses the tyrosine phosphorylation of paxillin during integrin signaling. PMID: 19917054
    28. a preformed signaling complex mediates GnRH-activated ERK phosphorylation of paxillin and FAK at focal adhesions in L beta T2 gonadotrope cells PMID: 19628583
    29. Hepatocyte growth factor induces ERK-dependent paxillin phosphorylation and regulates paxillin-focal adhesion kinase association PMID: 11784715
    30. linkage of caspase-mediated degradation to apoptosis in murine pro-B lymphocytes PMID: 11825902
    31. PP-2A regulation of paxillin phosphorylation may have a role in controlling tumor cell adherence and motility. PMID: 12198769
    32. our results clearly dissociate EGFR transactivation from the tyrosine phosphorylation of FAK and paxillin induced by multiple GPCR agonists. PMID: 12548551
    33. Ras-induced serine phosphorylation of Pxn is mediated by the Raf-MEK-ERK pathway. PMID: 12837288
    34. Data show that paxillin-dependent ERK activation at sites of cell-matrix interaction is critical for hepatocyte growth factor-stimulated epithelial morphogenesis. PMID: 14636584
    35. paxillin has a role in EphB1-mediated cell migration PMID: 15107421
    36. Vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction. PMID: 15138291
    37. Paxillin preferenailly associates with high affinity alpha4 integrins that are constituitively expressed by leukocytes in spleen and peritoneal cavity PMID: 15242880
    38. paxillin tyrosine 31 and 118 phosphorylation controls polarization and motility of lymphoid cells and is PMA-sensitive PMID: 15252114
    39. paxillin-MAPK complex serves as a central regulator of HGF-stimulated FAK and Rac activation in the vicinity of focal adhesions PMID: 15494312
    40. paxillin is independently phosphorylated by both tyrosine kinase(s) and ERK downstream of the TCR and localized both at the microtubule organizing center and at the target cell contact point suggests an important role for paxillin in CTL-mediated killing PMID: 16339552
    41. Phosphorylation of paxillin on serines 126 and 130, which is mediated by an ERK/GSK-3 dual-kinase mechanism, plays an important role in cytoskeletal rearrangement. PMID: 16537926
    42. paxillin,the multifunctional focal adhesion protein, is phosphorylated at Ser 178 by upregulation of the Rac1/Cdc42/JNK cascade. Conversely, transfection of the paxillin construct harboring the Ser 178-to-Ala mutation into cells inhibits neurite extension PMID: 16814769
    43. Invadopodia ring expansion is controlled by paxillin phosphorylations on tyrosine 31 and 118, which allows invadopodia disassembly. PMID: 18045996
    44. Paxillin is localised to the 'poles' of elongated osteocyte cell bodies in unidirectionally loaded mouse fibulae and multidirectionally loaded calvariae. PMID: 18187040
    45. FAK interactions with paxillin and SH2-domain proteins are required for anoikis suppression PMID: 19126677
    46. Abl differentially regulates Crk-associated substrate, vinculin, and paxillin in arterial vessels PMID: 19542491
    47. Paxillin is required for the tyrosine phosphorylation of FAK in ES cells. PMID: 11791180

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.
  • 蛋白家族：
    Paxillin family
  • 数据库链接：

    KEGG: mmu:19303

    STRING: 10090.ENSMUSP00000083709

    UniGene: Mm.18714