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  4. Recombinant Mouse Perilipin-1 (Plin1)

Recombinant Mouse Perilipin-1 (Plin1)

  • 货号：
    CSB-YP806541MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806541MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806541MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806541MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806541MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Plin1; Peri; Plin; Perilipin-1; Lipid droplet-associated protein; Perilipin A
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-517
  • 氨基酸序列
    MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA LGSIEKVVEF LLPPDKESAP SSGRQRTQKA PKAKPSLVRR VSTLANTLSR HTMQTTAWAL KQGHSLAMWI PGVAPLSSLA QWGASAAMQV VSRRQSEVRV PWLHNLAASQ DESHDDQTDT EGEETDDEEE EEESEAEENV LREVTALPNP RGLLGGVVHT VQNTLRNTIS AVTWAPAAVL GTVGRILHLT PAQAVSSTKG RAMSLSDALK GVTDNVVDTV VHYVPLPRLS LMEPESEFRD IDNPSAEAER KGSGARPASP ESTPRPGQPR GSLRSVRGLS APSCPGLDDK TEASARPGFL AMPREKPARR VSDSFFRPSV MEPILGRAQY SQLRKKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
  • 基因功能参考文献：
    1. This mouse model might mimic and explain the pathogenesis of hepatosteatosis occurring in two typical disorders of adipose tissue dysfunction, obesity and lipodystrophy, particularly in lipodystrophic patients with Plin1 mutation. PMID: 29191637
    2. IP6K1 is a novel regulator of PLIN1 mediated lipolysis PMID: 27373682
    3. ex vivo studies in human primary adipocytes, demonstrate that perilipin 1 binds to AQP7, and that catecholamine activated protein kinase A phosphorylates the N-terminus of AQP7, thereby reducing complex formation PMID: 27832861
    4. Here, we identify synthetic ligands that release ABHD5 from PLIN1 or PLIN5 without PKA activation and rapidly activate adipocyte and muscle lipolysis. PMID: 26411340
    5. Heterozygous Plin1+/- SVCs were able to develop lipid droplets, with both the number and size more than in Plin1-/- SVCs but less than in Plin1+/+ SVCs, indicating that Plin1 haploinsufficiency accounts for attenuated adipogenesis. PMID: 25695774
    6. Plin1 deficiency in bone marrow-derived cells may be responsible for reduced atherosclerotic lesions in the mice PMID: 25855981
    7. Perilipin+ embryonic preadipocytes actively proliferate along growing vasculatures for adipose expansion. PMID: 26243869
    8. Data suggest cardiotrophin-1 up-regulates lipolysis in adipocytes via 1) induction of Plin1, 2) activation of hormone sensitive lipase (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of G0S2). PMID: 25351614
    9. Adipocytes of Plin1/ mice showed robust basal lipolysis and fatty acid efflux to the plasma. Such adipose tissue dysfunctions accounted for the ectopic lipid accumulation and enhanced fatty acid transport and oxidation in Plin1/ hearts. PMID: 25416668
    10. QRFP-43 attenuates lipolysis by preventing the formation of an active complex between perilipin A, caveolin-1, the catalytic subunit of protein kinase and hormone-sensitive lipase on lipid droplets. PMID: 25677823
    11. ESR1 regulates ATGL and perilipin-mediated lipid metabolism and droplet size in femurs from mice. PMID: 23296636
    12. Collectively these data suggest that whereas perilipin 1 potently suppresses basal lipolysis in adipocytes, perilipins 2 and 3 facilitate higher rates of basal lipolysis PMID: 24927580
    13. these findings indicated that PLIN1 disruption leads to the increase of round spermatid-containing seminiferous tubules at the meiotic stage of the first wave of spermatogenesis through regulating spermatogenic related genes. PMID: 24727056
    14. Data indicate that perilipin (plin) deficiency suppresses sterol regulatory element-binding protein-1 (SREBP-1) activation in white adipose tissue (WAT). PMID: 23734208
    15. Perilipin1 (Plin1), another adipocyte-specific lipid droplet-associated protein, is an Fsp27 activator. PMID: 23481402
    16. FSP27 acts to constitutively limit the lipid droplet presence of ATGL, perilipin 1 plays an essential role in mediating the response of ATGL action to beta-adrenergic hormones. PMID: 23204327
    17. the reduction of perilipin1 in white adipose tissues may at least in part contribute to conjugated linoleic acid -mediated alternation of lipolysis of white adipose tissues. PMID: 22609209
    18. Perilipin 1 ablation has limited consequences on energy balance. It does not impair exercise performance; fatty acids mobilization during exercise is not impaired, whereas their oxidation is enhanced PMID: 21944269
    19. Lipolysis is differently regulated between perigonadal and mesenteric adipocytes, and these depot-specific differences might be explained by altered regulation of G0S2 and/or perilipin. PMID: 21989031
    20. Results support an atheroprotective role of perilipin1 since the extent of atheroma lesions was increased in Ldlr-/- mice crossed with Plin1-/- mice. PMID: 21943217
    21. Findings demonstrate that overexpression of PeriA in white adipocytes reduces lipid droplet size by decreasing FSP27 expression and thereby inducing a brown adipose tissue-like phenotype. PMID: 21103377
    22. a novel role for perilipin expression in adipose tissue metabolism and regulation of obesity and its metabolic complications PMID: 19797618
    23. The major metabolic parameters were examined in 129/SvEv perilipin null mice fasted for 4 h and observed increased plasma concentrations of free fatty acids, glycerol, glucose, and insulin. PMID: 20091459
    24. Data demonstrate that HSL can interact with an N-terminal region located between amino acids 141 and 200 of Plin A as well as with a C-terminal region located between amino acids 406 and 480. PMID: 19515989
    25. Results demonstrate that Plin binds Abhd5 with high affinity and thereby suppresses the interaction of Abhd5 with Atgl. PMID: 19850935
    26. perilipin expression and phosphorylation state are critical regulators of lipid storage and hydrolysis in ACS1/FATP1 cells. PMID: 11751901
    27. the central 25% of the perilipin A sequence contains all of the amino acids necessary to target and anchor the protein to lipid droplets PMID: 12407111
    28. phosphorylated perilipin has a role in hormone-sensitive lipase translocation PMID: 12832420
    29. identify domains of Peri A that act as suppressors of basal lipolysis and domains that act as enhancers of protein kinase A-stimulated lipolysis by hormone-sensitive lipase(HSL) and/or non-HSL PMID: 14527948
    30. gene-expression profiling of white adipose tissue, liver, heart, skeletal muscle, and kidney of perilipin deficient mice compared with wild-type littermates PMID: 14578284
    31. amino and carboxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage PMID: 14610073
    32. perilipin is regulated by PPARgamma in adipocytes PMID: 14704148
    33. propose that peroxisome proliferator-activated receptor gamma(PPAR)plays a significant role in the transcriptional regulation of murine perilipin gene via the PPAR response element in its promoter PMID: 14726448
    34. perilipin interacts with CGI-58 and is localized to lipid droplets PMID: 15136565
    35. the metabolic adaptations associated with the constitutional lipolysis in plin(-/-) mice that allowed the mice to continue to exhibit normal glucose tolerance in the presence of peripheral insulin resistance PMID: 15197189
    36. CGI-58 binds to perilipin A-coated lipid droplets in a manner that is dependent upon the metabolic status of the adipocyte and the activity of cAMP-dependent protein kinase PMID: 15292255
    37. three hydrophobic sequences play a major role in targeting and anchoring perilipins to lipid droplets PMID: 15342676
    38. Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase PMID: 16243839
    39. Transfected mouse perilipin is degraded through proteasome, a process that is inhibited by MG132 or ALLN and blunted by the addition of oleic acid. PMID: 16448845
    40. PKA-dependent perilipin phosphorylation facilitates (either direct or indirect) perilipin interaction with lipid droplet-associated hormone-specific lipase PMID: 16595669
    41. Peri A regulates ATGL-dependent lipolysis and serine 517 is the Peri A PKA site essential for this regulation. PMID: 17114792
    42. Proteolysis of perilipin involving the lysosomal protein degradation machinery may constitute a novel mechanism for enhancing adipocyte lipolysis after nelfinavir administration. PMID: 17488708
    43. Perilipin, which was thought to be characteristic for lipid droplets of adipocytes and steroidogenic cells, becomes de novo expressed in hepatocytes of human, mouse, and cattle liver. PMID: 18393390
    44. evidence is presented for perilipin expression in rat, mouse, and human islets of Langerhans PMID: 19299455
    45. RORalpha is a novel negative regulator of adipocyte differentiation that acts through dual mechanisms. PMID: 19324970

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  • 亚细胞定位：
    Endoplasmic reticulum. Lipid droplet.
  • 蛋白家族：
    Perilipin family
  • 数据库链接：

    KEGG: mmu:103968

    STRING: 10090.ENSMUSP00000032762

    UniGene: Mm.254917