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  4. Recombinant Mouse Phenylethanolamine N-methyltransferase (Pnmt)

Recombinant Mouse Phenylethanolamine N-methyltransferase (Pnmt)

  • 中文名称：
    小鼠Pnmt重组蛋白
  • 货号：
    CSB-YP018274MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pnmt重组蛋白
  • 货号：
    CSB-EP018274MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pnmt重组蛋白
  • 货号：
    CSB-EP018274MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pnmt重组蛋白
  • 货号：
    CSB-BP018274MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pnmt重组蛋白
  • 货号：
    CSB-MP018274MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pnmt
  • Uniprot No.：
  • 别名：
    Pnmt; Phenylethanolamine N-methyltransferase; PNMTase; EC 2.1.1.28; Noradrenaline N-methyltransferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-295
  • 氨基酸序列
    MNGGSDLKHA TGSGSDPKHA AEMDPDSDAG QVAVALAYQR FEPRAYLRNN YAPPRGDLSN PDGVGPWKLR CMAQVFATGE VSGRVLIDIG SGPTIYQLLS ACAHFEDITM TDFLEVNRQE LGLWLREEPG AFDWSVYSQH ACLIEDKGES WQEKERQLRA RVKRVLPIDV HKPQPLGTPS LVPLPADALV SAFCLEAVSP DLTSFQRALH HITTLLRPGG HLLLIGALEE SWYLAGEARL SVVPVSEEEV REALVLGGYE VRELRTYIMP AHLCTGVDDV KGIFFAWAQK MEVQV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Converts noradrenaline to adrenaline.
  • 基因功能参考文献：
    1. Data suggest that Pnmt plays role in fear response and startle reactivity; compared with wild-type mice, Pnmt-knockout mice exhibit reduced contextual fear but normal cued fear. PMID: 23268986
    2. Although temperature was normal in Pnmt(-/-) mice, the brown fat response to cold was abnormal with no increase in Ucp-1 or Pgc-1alpha mRNA levels (but with an exaggerated cold-induced lipid loss from the tissue). PMID: 20619316
    3. surprisingly high levels of the epinephrine synthesizing enzyme phenylethanolamine N-methyl transferase (PNMT) were detected in the thymus of young mice. PMID: 12096893
    4. A truncated mouse phenylethanolamine N-methyltransferase splice variant has dominant-negative activity PMID: 12438096
    5. PNMT expression is regulated late in mouse gestation by AP2-alpha and glucocorticoids PMID: 12438097
    6. Glucocorticoid receptors form multimers of Pmnt independent of the DNA binding domain. PMID: 12933902
    7. clear, significant increase in aggression arising from PNMT overexpression PMID: 15824970
    8. Regulation of the PNMT gene expression in various compartments of heart includes both corticosterone-dependent and independent mechanisms. PMID: 16691441
    9. Transcriptional suppression by cAMP as a mechanism for regulating PNMT expression in both normal and neoplastic mouse chromaffin cells. PMID: 17196587
    10. Epinephrine is required for normal blood pressure and cardiac filling responses to stress but is not required for tachycardia during stress or normal cardiovascular function at rest in the knockout mouse. PMID: 17698731
    11. Neuronal nitric oxide synthase gene inactivation reduces the expression of vasopressin in the hypothalamic paraventricular nucleus and of PNMT in the adrenal gland of the mouse. PMID: 17853069

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  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
  • 组织特异性：
    Brain (pons and medulla oblongata), adrenal gland and retina.
  • 数据库链接：

    KEGG: mmu:18948

    STRING: 10090.ENSMUSP00000035549

    UniGene: Mm.57030