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  4. Recombinant Mouse Phosphatidylcholine-sterol acyltransferase (Lcat)

Recombinant Mouse Phosphatidylcholine-sterol acyltransferase (Lcat)

  • 货号：
    CSB-YP012783MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012783MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012783MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012783MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012783MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    LcatPhosphatidylcholine-sterol acyltransferase; EC 2.3.1.43; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-438
  • 氨基酸序列
    FWLLNV LFPPHTTPKA ELSNHTRPVI LVPGCLGNRL EAKLDKPDVV NWMCYRKTED FFTIWLDFNL FLPLGVDCWI DNTRIVYNHS SGRVSNAPGV QIRVPGFGKT ESVEYVDDNK LAGYLHTLVQ NLVNNGYVRD ETVRAAPYDW RLAPHQQDEY YKKLAGLVEE MYAAYGKPVF LIGHSLGCLH VLHFLLRQPQ SWKDHFIDGF ISLGAPWGGS IKAMRILASG DNQGIPILSN IKLKEEQRIT TTSPWMLPAP HVWPEDHVFI STPNFNYTVQ DFERFFTDLH FEEGWHMFLQ SRDLLERLPA PGVEVYCLYG VGRPTPHTYI YDHNFPYKDP VAALYEDGDD TVATRSTELC GQWQGRQSQP VHLLPMNETD HLNMVFSNKT LEHINAILLG AYRTPKSPAA SPSPPPPE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters.
  • 基因功能参考文献：
    1. Taken together, these results suggest that apoE-containing discoidal HDLs do not require LCAT-dependent maturation to mediate efficient Abeta clearance PMID: 24950691
    2. increased cholesterol esterification by LCAT is atheroprotective PMID: 25964513
    3. Gene transfer of WT LCAT in LCAT(-/-) mice increased 11.8-fold the plasma cholesterol, whereas the LCAT[T147I] and LCAT[P274S] mutants caused a 5.2- and 2.9-fold increase, respectively. PMID: 25948084
    4. DYRK1A overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein A-I levels. PMID: 23920041
    5. adrenal glucocorticoid function in LCAT knockout (KO) mice PMID: 23178225
    6. a novel function of apoA-IV in the biogenesis of discrete HDL-A-IV particles with the participation of ABCA1 and LCAT PMID: 23132909
    7. Studies suggest that absence of lecithin cholesterol acyltransferase (LCAT) may protect against insulin resistance, diabetes and obesity. PMID: 22326749
    8. Data show that LCAT activity was significantly higher in long chain base biosynthesis protein 2 (Sptlc2)+/- and sphingomyelin synthase 2 (Sms2)-/- mice, but markedly lower in ApoE-/- and Ldlr-/- mice. PMID: 22370449
    9. Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice. PMID: 22500017
    10. LCAT deficiency confers gender-specific protection against diet-induced obesity and insulin resistance at least in part through regulation in UPR, white adipose tissue adipogenesis, and brown adipocyte partitioning PMID: 21454561
    11. Oxidative stress is markedly elevated in lecithin:cholesterol acyltransferase-deficient mice and is paradoxically reversed in the apolipoprotein E knockout background in association with a reduction in atherosclerosis PMID: 11809774
    12. LCAT contributes significantly to the cholesteryl ester (CE) fatty acid pool of apoB lipoprotein and is the only source of plasma long chain polyunsaturated CE in LDL receptor and apoE knockout mice. PMID: 11893779
    13. hypertriglyceridemia and the improved fasting glucose phenotype seen in the lecithin-cholesterol acyltransferase and ldl receptor double knockout mice are in part attributable to up-regulation of hepatic sterol regulatory element binding factor-1c gene PMID: 14668345
    14. LCAT has a role in regulating plasma HDL cholesterol levels and biliary cholesterol excretion PMID: 15251433
    15. apoE is a more significant activator of LCAT than apoA-I on mouse apoB lipoproteins PMID: 15654758
    16. ACAT2 provides core CE of newly secreted VLDL, whereas LCAT adds CE during LDL particle formation PMID: 15805543
    17. In LCAT deficient mice, alterations in hepatic expression of the gene for fads2, soat2, and dgat2, results in changes in total hepatic and cholesterol ester-associated polyunsaturated fatty acids. PMID: 16105858
    18. LCAT deficiency induces complex alterations in hepatic signal transduction cascades. PMID: 17551001
    19. These data show that brain LCAT esterifies cholesterol on glial-derived apoE-lipoproteins, and influences CSF apoE and apoA-I levels. PMID: 19065001
    20. LCAT overexpression does not promote an increased rate of macrophage reverse cholesterol transport. PMID: 19564558
    21. Cholesterol homeostasis was examined in mice with the two major cholesterol transport pathways for catabolism interrupted by disrupting abca1, lcat, or both. PMID: 19729076

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 组织特异性：
    Detected in blood plasma. Produced and secreted by astrocytes (at protein level). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neur
  • 数据库链接：

    KEGG: mmu:16816

    STRING: 10090.ENSMUSP00000038232

    UniGene: Mm.1593