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  4. Recombinant Mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (Pten)

Recombinant Mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (Pten)

  • 货号：
    CSB-YP018964MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018964MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018964MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018964MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018964MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pten; Mmac1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-403
  • 氨基酸序列
    TAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHSQI TKV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability.
  • 基因功能参考文献：
    1. The results of the present study suggest that PTEN deficiency mobilizes a variety of genes critical for cancer cell survival and host immune evasion. PMID: 30226608
    2. PTEN is a negative regulator of PI3K and BMP-7 increased PTEN expression in vivo and in vitro. These data demonstrate an important mechanism by which BMP-7 orchestrates renal protection through Akt inhibition and highlights Akt inhibitors as anti-fibrotic therapeutics. PMID: 28923783
    3. This study show here that deleting PTEN in the sensorimotor cortex of adult mice leads to progressive growth of cell bodies, dendrites, and axons of cortical neurons that continues for at least 12months post-deletion. PMID: 29337147
    4. The findings of this study indicated that co-deletion of PTEN and SOCS3 results in modest but measureable enhancement of early regeneration of DRG axons following crush injury. PMID: 29458059
    5. these results indicate that miR-21a negatively modulates two tumor suppressor genes, miR-200c and PTEN, thereby promoting M2 macrophage transformation PMID: 29359349
    6. Stromal PTEN determines mammary epithelial response to radiotherapy. PMID: 30018330
    7. MALAT1 knockdown attenuated myocardial apoptosis by suppressing Pten expression by sponging miR-320 in mouse acute myocarcial infarction model. PMID: 29990866
    8. Pten is essential for craniofacial morphogenesis in mice. Pten acts via modulating PI3K/Akt activity PMID: 29115005
    9. Pten plays roles in maintaining satellite cells quiescence. PMID: 28094257
    10. CK2 inhibition restores PTEN nuclear distribution and DNA repair activities and impairs tumour but not normal cell survival. PMID: 28094268
    11. Data suggest that Mps One binder 1 (MOB1) acts as a necessary regulator in PTEN-GSK3beta axis that controls neurite outgrowth after spinal cord injury (SCI). PMID: 30069702
    12. These findings demonstrate that deletion of NS-Pten results in significant decreases in vocalizations across both sexes. Additionally, our findings indicate that the aberrant vocalizations and increased call duration seen in other mTOR models are also present in NS-Pten knockout mice. Our study provides evidence of a connection between hyperactive mTOR signaling and neonatal ultrasonic vocalization. PMID: 29201556
    13. These data demonstrate the functional interplay between PTEN and EG5 in controlling mitotic spindle structure and chromosome behaviour during mitosis. PMID: 27492783
    14. These findings implicate a molecular disinhibitory mechanism driving the establishment of perisomatic inhibition whereby visual experience enhances Pten signalling, resulting in the suppression of EphB4 expression; this relieves a native synaptic repulsion between PV cells and pyramidal neurons, thereby promoting the assembly of perisomatic inhibition. PMID: 27611660
    15. miR-19 suppresses the expression of Pten and plays a key role in regulating B-cell tolerance. PMID: 27481093
    16. The overexpression of PTEN concomitant with Livin gene silencing was confirmed as a feasible and effective in vitro and in vivo gene modulation method, which may represent a potential therapeutic strategy for the treatment of Gastric Cancer. PMID: 29436592
    17. Deletion of the mTOR negative regulator, PTEN, from a subset of hippocampal dentate granule impairs dendritic patterning, increases excitatory input and decreases inhibitory input. Furthermore, while granule cells from female mice receive more excitatory and inhibitory input than males, PTEN deletion produces mostly similar changes in both sexes. PMID: 28855130
    18. Depletion of PTEN leads to elevated levels of stable and post-translationally modified (detyrosinated) microtubules in fibroblasts and developing neurons. PTEN depletion enhanced axon outgrowth, which was rescued by reducing the level of detyrosinated microtubules. PMID: 29617365
    19. USP10 directly interacted with and stabilized PTEN via deubiquitination. PMID: 28852924
    20. It has been observed that PTEN expression downregulated by p53 deficiency induced the activation of STAT3 in autoimmune arthritis. PMID: 27708408
    21. MiRNA-20a may have great potential as therapeutic target for coronary artery disease, since its participation can induce alteration of functional genes as well as PTEN. PMID: 28888922
    22. a T cell-specific Stat6/Pten axis links cold exposure or ADRB3 stimulation with Foxp3(+) Treg induction and adipose tissue function. PMID: 28877454
    23. Neuron-subset specific (NS)-Pten heterozygous (HT) and wildtype (WT) adult mice. PMID: 27819284
    24. PTEN ablation led to downregulation of BMI-1, a critical regulator of adult stem cell self-renewal, and elevated senescence, suggesting the presence of a protective system that prevents transformation. Short- and long-term PTEN depletion followed by activated BMAL1, a core clock protein, contributed to accumulation of hair follicle stem cells. PMID: 28602615
    25. cross talk between miR-214 and PTEN attenuated glomerular hypertrophy under diabetic conditions in vivo and in vitro. Therefore, miR-214 may represent a novel therapeutic target for diabetic nephropathy. PMID: 27549568
    26. Here, we provide novel insights into treatment response for the antiandrogen abiraterone by analyses of a genetically engineered mouse (GEM) model with combined inactivation of Trp53 and Pten, which are frequently comutated in human castration-resistant prostate cancer (CRPC). PMID: 28411207
    27. Pten is required for proamniotic cavity formation by establishing polarity for epiblast cells to form a rosette that expands into the proamniotic lumen, rather than facilitating apoptosis to create the cavity PMID: 28387983
    28. Here, we show that cabozantinib rapidly eradicates invasive, poorly differentiated PTEN/p53-deficient murine prostate cancer. This was associated with enhanced release of neutrophil chemotactic factors from tumor cells, including CXCL12 and HMGB1, resulting in robust infiltration of neutrophils into the tumor. PMID: 28274958
    29. Here we show that radiation-resistant cells and tumors derived from a Pten/Trp53-deficient mouse model of advanced prostate cancer are rendered radiation sensitive following treatment with NanoOlaparib, a lipid-based injectable nanoformulation of olaparib. PMID: 28500233
    30. CaMKII is a target of PTENalpha phosphatase and that PTENalpha is an essential element in the molecular regulation of neural activity. PMID: 28636948
    31. PTEN-beta-catenin signaling is a novel regulator involved in modulating Treg development and may lead to a potential therapeutic target in liver ischemia/reperfusion injury PMID: 28152578
    32. Loss of PTEN expression is associated with ovarian tumor cells originating in the fallopian tube stroma. PMID: 27602775
    33. The mice of Mutan of pten showed the neurons of brain exhibit excessive PI3K/Akt/mTOR signaling activity, enlarged somas and increased dendritic arborization. PMID: 28457820
    34. Increased expression of miR-214 suppresses PTEN expression and represses Bim1 expression. PMID: 27894079
    35. In summary, we show that Pten loss per se in Lgr5+ intestinal stem cells is not required either as a tumor suppressor or for maintaining intestinal homeostasis when Apc is functional, even when combined with obesity. PMID: 28351943
    36. PTEN reduces growth factor secretion by Kupffer cells. PMID: 28542148
    37. High intensity focused ultrasound exposure can inhibit AKT-mediated melanoma metastasis via miR-21 inhibition to restore PTEN expression PMID: 27391071
    38. Ultimately, our study illustrated that MEG3 exerts its role via miR-21/PTEN axis in human pulmonary artery smooth muscle cell under both normal and hypoxia conditions. PMID: 29198701
    39. The present findings demonstrate that selective deletion of PTEN from a subset of granule cells reproduces hippocampal pathophysiologies evident in traditional models of temporal lobe epilepsy. PMID: 27597527
    40. Up to 20% of the subset of individuals with ASD and macrocephaly (head circumference >2 standard deviations above the mean) have mutations in the gene Pten (phosphatase and tensin homolog). PMID: 27220363
    41. ompounding a previously described Bmi1-transgene and Pten-deficiency prostate cancer mouse model with the Ezh2 transgene did not enhance tumour progression or drive metastasis formation. In conclusion, we here report the generation of a wildtype Ezh2 overexpression mouse model that allows for intravital surveillance of tissues with activated transgene PMID: 27807665
    42. In conclusion, our data indicated that miR-26a potentially contributes to the beta-cell dysfunction in T2DM, and miR-26a may be a new therapeutic strategy against T2DM. PMID: 29191656
    43. CFTR attaches tumor suppressor PTEN to the membrane and promotes anti Pseudomonas aeruginosa immunity. PMID: 29246444
    44. These findings indicate that PTEN not only targets tumor cells themselves by impacting cell behaviors, but also blocks osteoclast-mediated bone destruction, leading to interruption of the vicious cycle during osteosarcomagenesis PMID: 28106296
    45. further molecular mechanisms of HOTAIR action in cardiac hypertrophy (CH) demonstrated that HOTAIR may act as a competing endogenous RNA (ceRNA) for miR-19, thereby modulating the dis-inhibition of its endogenous target PTEN and playing an important role in inhibiting CH progress. PMID: 28316060
    46. Setdb1 regulates PTEN/AKT/FOXO1 pathway to inhibit Spermatogonial stem cells apoptosis. PMID: 28890329
    47. phosphorylated-Pten promotes the proliferation and differentiation of hematopoietic stem cells. PMID: 27096933
    48. Results indicate miR-130a as an oncogenic miRNA that targets PTEN to drive malignant cell survival and tumor growth. PMID: 28935812
    49. PTEN loss and activation of K-RAS and beta-catenin cooperate to accelerate prostate tumourigenesis. PMID: 29134654
    50. Collectively, these findings provide a mechanistic account of how Rac1-dependent membrane raft localization regulates differential activation of distinct phosphoinositide 3-kinase isoforms and offer insight into why PTEN-deficient cancers depend on p110beta. PMID: 27700986

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, PML body.
  • 数据库链接：

    KEGG: mmu:19211

    STRING: 10090.ENSMUSP00000013807

    UniGene: Mm.245395