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  4. Recombinant Mouse Platelet-derived growth factor receptor beta (Pdgfrb), partial

Recombinant Mouse Platelet-derived growth factor receptor beta (Pdgfrb), partial

  • 货号：
    CSB-YP017713MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017713MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017713MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017713MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017713MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pdgfrb; Pdgfr; Pdgfr1; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; EC 2.7.10.1; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD antigen CD140b
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. Required for normal development of the cardiovascular system. Required for normal recruitment of pericytes (mesangial cells) in the kidney glomerulus, and for normal formation of a branched network of capillaries in kidney glomeruli. Promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its cognate ligands - homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFD -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1 and NCK1. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to the activation of the AKT1 signaling pathway. Phosphorylation of SHC1, or of the C-terminus of PTPN11, creates a binding site for GRB2, resulting in the activation of HRAS, RAF1 and down-stream MAP kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation and activation of SRC family kinases. Promotes phosphorylation of PDCD6IP/ALIX and STAM. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
  • 基因功能参考文献：
    1. PDGFRbeta was activated around the injury site after experimental traumatic brain injury (TBI), and primarily expressed in astrocytes, microglia, OPC and leukocytes in the boundary of the lesion site, suggesting PDGFRbeta was involved in glial scar formation. Then the PDGFR inhibitor (AG1296) was administered following TBI. Reactive astrocytes were significantly inhibited in AG1296-treated mice. PMID: 28684344
    2. Increasing the adipogenic capacity of Pdgfrbeta(+) precursors through Pparg overexpression results in healthy visceral white adipose tissue expansion in obesity and adiponectin-dependent improvements in glucose homeostasis. PMID: 29497032
    3. The data of this study revealed an acute accumulation of PDGFRbeta+ BBB-related cells in degenerating brain areas, which can be long lasting, suggesting an active role for PDGFRbeta-signaling in blood vessel and post-injury tissue recovery. PMID: 27778377
    4. Beta platelet-derived growth factor is induced in hepatic stellate cells (HSCs) following surgical partial hepatectomy, and its deletion in HSCs leads to prolonged liver injury in mice. However, there is no significant difference in liver regeneration. PMID: 26256287
    5. The results provide functional evidence that elevated PDGFRbeta signaling causes tissue wasting or overgrowth reminiscent of human genetic syndromes and that the STAT1 pathway is a crucial modulator of this phenotypic spectrum. PMID: 28924035
    6. Identify PDGFRbeta as a driver in activating Akt/mTORC1 nexus for high glucose-mediated expression of collagen I (alpha2) in proximal tubular epithelial cells, which contributes to tubulointerstitial fibrosis in diabetic nephropathy. PMID: 28424212
    7. Data show that three platelet-derived growth factor receptor beta (PDGFRB) mutants (R561C, P660T and N666K) were able to transform NIH3T3 and Ba/F3 cells to different extents. PMID: 26455322
    8. data therefore collectively suggest that upon TGFbeta stimulation, SP1 recruits SMAD2 to the promoter of Pdgfrb to up-regulate its expression and thus Pdgfrb is a direct downstream target of the TGFbeta/SMAD2 signaling PMID: 28426709
    9. PdgfrbetaF7/F7 mice between 4-6 and 36-48 weeks of age developed a region-dependent loss in pericyte coverage (22-46, 24-44 and 4-31%) and cell numbers (36-49, 34-64 and 11-36%), reduction in capillary length (20-39, 13-46 and 1-30%), and an increase in extravascular fibrinogen-derived deposits (3.4-5.2, 2.8-4.1 and 0-3.6-fold) demonstrating BBB breakdown in the cortex, hippocampus and thalamus, respectively. PMID: 28441414
    10. there is great possibility that EPCs overexpressing PDGFR-beta enhanc VSMC apoptosis and suppress VSMC migration by competitive consumption of PDGF-BB in the early phase after carotid artery injury in mice. PMID: 27619504
    11. Data show that oligodendrocyte transcription factor 2 (Olig2) deletion causes platelet-derived growth factor receptor (PDGFR) downregulation and reciprocal epidermal growth factor receptor (EGFR) upregulation. PMID: 27165742
    12. Decreased expression of PDGFR-beta in vascular smooth muscle cells was associated with thrombosis in vivo. PMID: 27198239
    13. Our results highlight the need for careful interpretation of lineage tracing using constitutive Cre lines that cannot discriminate active from historical expression. The early vascular targeting by the Pdgfrb-Cre also warrants consideration for its use in studies of mural cells PMID: 27060598
    14. Pdgfrbeta(+) cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. PMID: 26626462
    15. Results suggested that increased bFGF upregulates the expression of PDGFRbeta and may enhance PDGFRbeta-mediated pericyte functions after brain ischemia PMID: 26569132
    16. The role of the S1PR3/PDGFR-beta/Akt pathway in sphingosine-1-phosphate -induced endothelial progenitor cells migration and angiogenesis PMID: 26208383
    17. Increased PDGFRbeta signalling promotes advanced plaque formation at novel sites in the thoracic aorta and coronary arteries. PMID: 26183159
    18. This study demonstrated that PDGFRbeta-expressing cells appear to arise from vascular wall pericytes in peri-infarct areas and then extend toward the ischemic core while producing ECM proteins, fibronectin and collagen type I, after MCAO. PMID: 25510317
    19. These results suggest that gonocyte and F9 cell differentiation is regulated via cross talk between retinoic acid and PDGFRs using different downstream pathways. PMID: 25380237
    20. The study shows that PDGFRB and FGFR2 mediate endothelial cell differentiation capability of triple negative breast carcinoma. PMID: 24747080
    21. Radial glia require PDGFD-PDGFRbeta signalling in human but not mouse neocortex PMID: 25391964
    22. The study demonstrates changes in pericyte expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs by chronic osmotic stimulation. PMID: 23629811
    23. the expression and function of PDGF-Rbeta in lower esophageal sphincter (LES)circular smooth muscle (CSM) from W/W(v) mice is impaired, providing further evidence that LES CSM is abnormal in W/W(v) mutants. PMID: 24383871
    24. These results are the first to identify EphB4 and its cross-talk with PDGFRbeta as unexpected vital determinants of tumor cell survival in alveolar rhabdomyosarcoma. PMID: 24733895
    25. ephrin-B2 is an important regulator of PDGFRbeta endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor PMID: 24298057
    26. Suggest that myocardin regulates vascular injury response via miR-24/miR-29a/PDGFRB pathway. PMID: 23825366
    27. Selective activation of oxidized PTP1B by the thioredoxin system modulates PDGF-beta receptor tyrosine kinase signaling. PMID: 23901112
    28. These results suggest that PDGFR-beta signaling is important for the self-renewal and multipotency of neural stem cells. PMID: 23454370
    29. These results suggest that PDGFRB may contribute to the aggressive phenotype of colorectal tumors with mesenchymal properties, most likely downstream of platelet activation and TGFB signaling. PMID: 23441134
    30. The Pdgfrb(redeye/redeye) mice exhibit all of the features of nonproliferative diabetic retinopathy, including retinal neurodegeneration. PMID: 23633653
    31. The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. PMID: 23651497
    32. determined that PDGF-regulated glycolysis occurs independent of PDGF-regulated proliferation but requires the activation of AKT, a known metabolic regulator in tumor PMID: 23322009
    33. cell proliferation induced by Wnt3a in osteoblastic cells is mediated by a dishevelled-dependent and beta-catenin-independent pathway, which involves the transactivation of PDGF receptors. PMID: 22927028
    34. Impairment in hippocampal long-term potentiation and in hippocampus-dependent memory formation are seen in PDGFR-beta knockout mice. PMID: 21997856
    35. findings demonstrate a novel capacity for p19(Arf) to control Pdgfrbeta expression by p53-dependent and -independent mechanisms involving RNA transcription and protein synthesis, respectively, to promote the vascular remodeling needed for normal vision PMID: 22907756
    36. Data indicate that the proteolytic processing of full-length PDGF-D is required for PDGF-D activation of preosteoclasts, and that beta-PDGFR is present in activated osteoclasts. PMID: 22158043
    37. Blocking the PDGFRbeta pathway with AG1295 markedly promotes osteoblast differentiation and matrix mineralization. PMID: 22890161
    38. beta-catenin transcriptional activity directly regulated endothelial expression of platelet-derived growth factor B, leading to mural cell recruitment thereby contributing to vascular quiescence PMID: 22908324
    39. a) PDGFR-beta could be used as a marker for TCs and b) TCs are presumably a transitional population in the complex process of mural cell recruitment during angiogenesis and vascular remodelling PMID: 22188481
    40. the involvement of CCL5 in the pathogenesis of colorectal carcinoma PMID: 22205974
    41. From 3 to 28 days after middle cerebral artery occlusion, postnatally induced systemic PDGFR-beta knockout mice (Esr-KO) exhibited the delayed recovery of body weight and behavior, and larger infarction volume than controls. PMID: 21952111
    42. Data indicate that signalling through both PDGFR-beta and CaMKII-alpha contributes to progression of diabetic nephropathy, with increase in oxidative stress and mesangial expansion. PMID: 21833587
    43. Data show a dramatic discrepancy between ROSA26 reporter activity and Pdgfrb promoter driven Cre dependent myc-tagged Cthrc1 transgene expression. PMID: 21557454
    44. Platelet-derived growth factor (PDGF)-BB produces NO-mediated relaxation and PDGF receptor beta-dependent tonic contraction in murine iliac lymph vessels. PMID: 21535294
    45. PDGFR-beta plays a role in the structure and function of kidney glomeruli in the mouse PMID: 20693161
    46. PDGFR-beta plays an important role in cognitive and socioemotional functions. PMID: 21437241
    47. A novel signaling pathway that links ATM via CREB to the transcription factor ZFHX3, which in turn promotes survival of neurons by inducing expression of platelet-derived growth factor receptor beta, is reported. PMID: 20876357
    48. Myocardin may contribute to the differentiation of CXCR4(+)/PDGFRbeta(+) progenitor cells into smooth muscle cells induced by hypoxia, which leads to the muscularization of alveolar arterioles. PMID: 20484220
    49. Data show that in high-fat-fed apolipoprotein E(-/-) mice, inhibition of PDGFRbeta tyrosine kinase activity leads to inhibition of GAG synthesis on vascular PGs and aortic lipid area. PMID: 20610572
    50. PDGFRbeta activates ADAM17 and promotes metalloproteinase-dependent cross-talk between the PDGFRbeta and epidermal growth factor receptor (EGFR) signaling pathways PMID: 20529858

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. Note=After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 组织特异性：
    Weakly expressed in glomerular mesangial cells and interstitial cells. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, increased expression in interstitial cells at day 4 and expression is markedly elevated at day 7
  • 数据库链接：

    KEGG: mmu:18596

    STRING: 10090.ENSMUSP00000025522

    UniGene: Mm.4146