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  4. Recombinant Mouse Polycomb group RING finger protein 2 (Pcgf2)

Recombinant Mouse Polycomb group RING finger protein 2 (Pcgf2)

  • 货号：
    CSB-YP017606MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017606MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017606MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017606MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017606MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pcgf2; Mel-18; Mel18; Rnf110; Zfp144; Znf144; Polycomb group RING finger protein 2; DNA-binding protein Mel-18; Melanoma nuclear protein 18; RING finger protein 110; Zinc finger protein 144; Zfp-144
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-342
  • 氨基酸序列
    MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA YPLTEVPNGS NEDRGEVLEQ EKGALGDDEI VSLSIEFYEG VRDREEKKNL TENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEILYEDE PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLPT VPTPSEGTNT SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP TPPSTAAGTT TATNGGTSNC LQTPSSTSRG RKMTVNGAPC PP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development (Probable). Regulates proliferation of early T progenitor cells by maintaining expression of HES1(PubMed:15728456). Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.
  • 基因功能参考文献：
    1. Data indicate that Bmi1 and Mel18 have opposing functions and are present in distinct complexes. PMID: 22634509
    2. Mel-18 controls the enrichment of tumor-initiating cells in side population fraction in mouse mammary gland cancer. PMID: 21970185
    3. Data show that Mel-18 and Ezh2 positively regulate the expression of Il17a and Il17f. PMID: 21674483
    4. Chemokine-mediated thymopoiesis is regulated by a mammalian Polycomb group gene, mel-18 PMID: 11750047
    5. involved in the specification of the anterior-posterior axis in mice PMID: 12183370
    6. the stoichiometry and/or equilibrium of subunits of the class II Polycomb complex containing Mel-18 might be regulated by changes in phosphorylation status via the PKC signaling pathway PMID: 12480532
    7. Results provide genetic evidence that Cited2 controls the expression of INK4a/ARF and fibroblast proliferation, at least in part via the polycomb-group genes Bmi1 and Mel18. PMID: 14560011
    8. Loss or knockdown of mel-18 leads to the expression of Hoxb4, an increase in the proportion of hematopoietic stem cells in G0 phase, and the subsequent promotion of HSC self-renewal. PMID: 15183898
    9. Mel-18 contributes to the maintenance of the active state of the Hes-1 gene as a cellular memory system, thereby supporting the expansion of early T lymphocyte progenitors. PMID: 15728456
    10. Sf3b1-Zfp144 protein interaction is essential for true Polycomb group proteins mediated repression of Hox genes. PMID: 15741318
    11. observed that expression of the PcG genes-bmi1 and mel-18-is correlated with self-renewal and differentiation of HSCs. Thus, it was suggested that the balance between Bmi1 and Mel-18 regulates self-renewal of HSCs PMID: 19409954

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  • 相关疾病：
    Probably related to tumorigenesis since it is expressed strongly in most tumor cell lines.
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in embryonic stem cells. Expressed in a variety of tumor cells and in neural tissues.
  • 数据库链接：

    KEGG: mmu:22658

    STRING: 10090.ENSMUSP00000018681

    UniGene: Mm.2418