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  4. Recombinant Mouse Profilin-1 (Pfn1)

Recombinant Mouse Profilin-1 (Pfn1)

  • 货号：
    CSB-YP017824MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017824MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017824MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017824MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017824MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pfn1; Profilin-1; Profilin I
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-140
  • 氨基酸序列
    AGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
  • 基因功能参考文献：
    1. Profilin1 is expressed in osteocytes and regulates cell shape, migration and bone mass. PMID: 28233307
    2. overexpression of profilin is sufficient to induce cardiomyocyte hypertrophy and sarcomeric remodelling, and silencing of profilin attenuates the hypertrophic response PMID: 26956799
    3. Pfn1 is a novel target of BMP and suppresses BMP-induced differentiation of osteoblasts at least in part via transcriptional event. PMID: 26271366
    4. knockdown of either profilin 1 or profilin 2a led to a significant decrease in cell spreading of astrocytes. Moreover, both isoforms proved to be crucial for forskolin-induced astrocytic stellation. PMID: 25629407
    5. Inactivation of profilin 1 impaired the radial migration of cerebellar granule neurons PMID: 25495756
    6. Megakaryocyte-specific Profilin1-deficiency alters microtubule stability and causes a Wiskott-Aldrich syndrome-like platelet defect. PMID: 25187265
    7. Depleting FMNL1, another Formin family member, resulted in reduced mDia1 expression, while RhoA inhibition did not alter mDia1 expression, which indicated that there was a FMNL1-mDia1-Profilin1 signaling pathway in mouse oocytes. PMID: 25447542
    8. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700464
    9. In glioblastomas endothelial cell-specific Pfn-1 phosphorylation elevates HIF-1alpha expression leading to vascular abnormalities and tumor progression. PMID: 24747440
    10. Pfn1 as a key effector of the integrin linked kinase/Rho/ROCK pathway which acts in parallel with integrin beta1/LCK/Rac1 and regulates Schwann cells lamellipodia formation, radial sorting and myelination during peripheral nervous system maturation. PMID: 24598164
    11. pfn1 at least partially acts through the axis of pfn1/Galpha13/EGR1 to regulate stem cell retention and metabolism in the bone marrow. PMID: 24385538
    12. findings indicate that pfn haploinsufficiency protects against diet-induced insulin resistance and inflammation by modulating white adipose tissue immune homeostasis. PMID: 23884883
    13. The results of this study found that the profilin1 is crucially important for brain development, but dispensable for the physiology of excitatory synapses. PMID: 22864186
    14. Recognition of profilin by Toll-like receptor 12 is critical for host resistance to Toxoplasma gondii. PMID: 23246311
    15. It was shown that activation of VEGF receptor kinase-2 and Src induces profilin 1 phosphorylation in the cell leading edge, promoting profilin 1 binding to actin and actin polymerization. Profilin 1 phosphorylation is critical for angiogenesis. PMID: 23000962
    16. Pfn1 is a critical molecule for the skeletal development, and this could be at least in part associated with the retardation of cell migration PMID: 22773831
    17. ACE2 deficiency enhances angiotensin II-mediated aortic profilin-1 expression, inflammation and peroxynitrite production. PMID: 22693641
    18. Loss of profilin1 had no adverse effects on the morphology and function of excitatory synapses. PMID: 22253883
    19. Profilin1 ablation from mouse brains leads to a cerebellar hypoplasia, aberrant organization of cerebellar cortex layers and ectopic Cerebellar granule neurons (CGNs). PMID: 22081137
    20. during elongation from profilin-actin, the dissociation of profilin from the growing barbed end is not coupled to Pi release or to ATP cleavage on the terminal subunit PMID: 21980262
    21. Microtubule-dependent localization of mRNA encoding the actin regulatory protein profilin to sites in mouse embryonic fibroblasts, which express enhanced actin polymerization. PMID: 20129697
    22. tumor suppressor function of PFN1 requires a functional actin binding site PMID: 14767055
    23. Upregulation of profilin mRNA in the hippocampus is induced by entorhinal deafferentation and profilin is involved in microglial activation associated with morphological change, migration, and phagocytic behavior of microglial cells. PMID: 15139017
    24. In cerebral cortex and hippocampus profilin I immunostaining was associated predominantly with dendrites and was present in a subset of dendritic spines. In contrast, profilin I in cerebellum was associated primarily with presynaptic structures. PMID: 15654839
    25. ATP hydrolysis control of filament assembly from profilin-actin requires formin PMID: 17210567
    26. pfn levels modulate processes critical for early atheroma formation and suggest that pfn heterozygosity confers atheroprotection through combined endothelial- and macrophage-dependent mechanisms PMID: 17615372
    27. profilin 1 has a role in vascular hypertrophy and hypertension PMID: 17942408
    28. Results demonstrate that profilin 1 is not required for actomyosin ring formation in dividing chondrocytes but necessary to generate sufficient force for abscission during late cytokinesis. PMID: 19262563

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Profilin family
  • 数据库链接：

    KEGG: mmu:18643

    STRING: 10090.ENSMUSP00000018437

    UniGene: Mm.2647