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  4. Recombinant Mouse Profilin-2 (Pfn2)

Recombinant Mouse Profilin-2 (Pfn2)

  • 货号：
    CSB-YP884933MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP884933MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP884933MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP884933MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP884933MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pfn2; Profilin-2; Profilin II
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-140
  • 氨基酸序列
    MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPVEIDM IVGKDREGFF TNGLTLGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
  • 基因功能参考文献：
    1. RARalpha competes with other PFN2a-binding proteins bearing PRMs and involved in actin filaments elongation. Consequently, the actin filament network is altered and MEFs adhesion is decreased. This novel role opens novel avenues for the understanding of pathologies characterized by increased levels of cytoplasmic RARalpha. PMID: 29158086
    2. Pfn2 expression is controlled by the iron regulatory proteins in vivo and that Pfn2 contributes to maintaining iron homeostasis in cell lines and mice. PMID: 28774878
    3. knockdown of profilin 1 or profilin 2a led to significant decrease in cell spreading of astrocytes. the knockdown of profilin 2a resulted in a significantly reduced morphological complexity of astrocytes in both dissociated and slice culture astrocytes. PMID: 25629407
    4. Hyper-phosphorylation of profilin2a is the molecular link between SMN and the ROCK pathway repressing neurite outgrowth in neuronal cells. PMID: 21920940
    5. Profilin II regulates the exocytosis of kainate glutamate receptors. PMID: 20937818
    6. Phosphatidylinositol 4,5-bisphosphate releases Pfn2 from the Pfn2-dynamin 1 complex as well as from the Pfn2-actin complex, suggesting that Pfn2 is diverging the phosphoinositide signaling pathway to actin polymerization as well as endocytosis PMID: 16319076
    7. Following NMDA receptor activation profilin 2 accumulates not only in dendritic spines, but also in the nucleus of hippocampal neurons via a process involving rearrangement of the actin cytoskeleton. PMID: 16716297
    8. results highlight a novel, profilin2-dependent pathway, regulating synaptic physiology, neuronal excitability, and complex behavior PMID: 17541406
    9. High-resolution structural analysis of mouse profilin 2a complex formation with two physiological ligands: the formin homology 1 domain of mDia1 and the proline-rich domain of VASP. PMID: 18001770

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Profilin family
  • 组织特异性：
    Isoform IIa is the main isoform and is abundant in brain. Isoform IIb is a minor isoform.
  • 数据库链接：

    KEGG: mmu:18645

    STRING: 10090.ENSMUSP00000068890

    UniGene: Mm.271744