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  4. Recombinant Mouse Proliferating cell nuclear antigen (Pcna)

Recombinant Mouse Proliferating cell nuclear antigen (Pcna)

  • 货号：
    CSB-YP017621MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017621MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017621MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017621MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017621MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pcna; Proliferating cell nuclear antigen; PCNA; Cyclin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-261
  • 氨基酸序列
    MFEARLIQGS ILKKVLEALK DLINEACWDV SSGGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVIKMP SGEFARICRD LSHIGDAVVI SCAKNGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVHLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEA S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
  • 基因功能参考文献：
    1. The first evidence for non-repair functions of MGMT in cell cycle and highlight the involvement of PCNA in MGMT downregulation, with p21 attenuating the process. PMID: 29510343
    2. e results showed mTet1 modified mGSCs had better self-renewal and proliferation ability than wild-type mGSCs, mTet1 could also up-regulate JMJD3 to decrease H3K27me3, which also showed to suppress the MEK-ERK pathway. Furthermore, Co-IP analysis demonstrated that TET1 interact with PCNA and HDAC1 by forming protein complexes to comprehensively regulate dairy goat mGSCs and spermatogenesis. PMID: 27857213
    3. work reveals that simulated microgravity promotes the apoptotic response through a combined modulation of the Uev1A/TICAM/TRAF/NF-kappaB-regulated apoptosis and the p53/PCNA- and ATM/ATR-Chk1/2-controlled DNA-damage response pathways. PMID: 26887372
    4. REV1 promote PCNA monoubiquitylation after UV radiation through interacting with ubiquitylated RAD18. PMID: 26795561
    5. PCNA was mainly localized in decidual-stromal cells. PMID: 26799792
    6. Luteolin may inhibit tumor angiogenesis and tumor cell proliferation by down-regulation of LFA- 3 and PCNA and up-regulation of ICAM-1 in tumor tissue of tumor-bearing mice, thereby achieving its anti-tumor effect. PMID: 26600503
    7. Data suggest Usp1 (ubiquitin specific peptidase 1) down-regulation by autocleavage is critical for Usp1 to exert role in DNA interstrand crosslink repair; Usp1 role is de-ubiquitination of Pcna and Fancd2 (Fanconi anemia complementation group D2). PMID: 26783108
    8. The aberrant DNA replication mediated by the PCNA-DNA pol-beta complex induces p53-dependent neuronal cell death PMID: 25184665
    9. Demonstrate that miR-376a regulates primordial follicle assembly by modulating the expression of Pcna. PMID: 24686458
    10. while interaction with PCNA was important for targeting p21 to the CRL4Cdt2 ligase re-localized to MVM replication centers PMID: 24699724
    11. Styrax japonica glycoprotein increased diethylnitrosamine-induced PCNA activity. PMID: 23060247
    12. This study identifies a critical role for PCNA in adipose tissue development, and for the first time identifies a role of the core DNA replication machinery at the interface between proliferation and differentiation. PMID: 23201573
    13. Seaweed pigment glycoprotein reduced the expression of PCNA and Bcl2, which reduced proliferation of hepatocarcinoma cells. PMID: 21560315
    14. These results suggest that the ncRNA is divergently transcribed from the bidirectional promoter, positively regulating the neighboring protein-coding Pcna gene transcription, and this regulatory function is somehow disrupted in cancer cells. PMID: 22228179
    15. these data support the existence of PCNA ubiquitination-dependent and -independent activation pathways of Poleta during somatic hypermutation and DNA damage tolerance. PMID: 21889916
    16. Taken together these results indicate that PCNA-ubiquitination is required for maximal translesion DNA synthesis. PMID: 21931560
    17. FFAA Fen1 mutation causes defective Pcna -coordinated Okazaki fragment maturation. PMID: 21383776
    18. PCNA was shown to be differentially expressed during during primordial follicle assembly in mouse ovaries PMID: 21253613
    19. data indicate that Rad1(K185) is not a functional counterpart of PCNA(K164). PMID: 21304913
    20. Grape procyanidins could promote PCNA protein expression in hepatic cells. PMID: 17424843
    21. High-dose 5-FU treatment can significantly increase the PCNA index in severely damaged small intestinal mucosa. PMID: 18159003
    22. there is a p53-independent function of MTA1 in DNA damage response via modulation of the p21 WAF1-proliferating cell nuclear antigen pathway PMID: 20071335
    23. Pcna is a direct downstream target of Hoxc8. PMID: 20097160
    24. VEGF, but not PCNA may play a role in tumor recurrence and metastasis following photodynamic therapy PMID: 19885588
    25. p53-mediated transcriptional repression plays a role in the spatial restriction of PCNA gene expression during normal renal development PMID: 12217864
    26. PCNA protein expression is regulated by p21 in mouse adult cardiomyocytes, which has implications for cardiomyocyte growth control PMID: 12509454
    27. PCNA, an S phase cell cycle marker, did not change in cells of the epidermal leading edge of the wound. PMID: 12791271
    28. Highest density of immunoreactive cells was observed in all animals in cuboidal cells around rupture site the day after ovulation. Total ovarian surface epithelium cells positive for PCNA in both the 8- and 12-mo animals was reduced. PMID: 15229142
    29. These findings provide evidence for the existence of a signal transduction pathway that links a rapamycin-activated type 2A protein phosphatase to the control of DNA synthesis, DNA repair, cell cycle, and cell death via PCNA. PMID: 15369799
    30. there is a differential regulation of NF-kappaB and PCNA proliferative activity in the splenic cell subsets after burn injury PMID: 15614135
    31. PCNA can serve as a marker of DNA repair and indirectly as an indicator of UV-B-induced damage, expression being time dependent and dose related PMID: 15623348
    32. Rb, p21Cip1, and PCNA have roles in cAMP-mediated inhibition of DNA replication and S phase progression PMID: 15647383
    33. REV3 and REV1 play major roles in recombination-independent repair of DNA interstrand cross-links mediated by monoubiquitinated PCNA PMID: 16571727
    34. These data demonstrate the importance of the FEN-1/PCNA complex in DNA replication and in the embryonic development of mice. PMID: 17283043
    35. PR8 treatment resulted in airway epithelial cell regeneration as suggested by proliferating PCNA-positive staining at 7 and 10 dpi. PMID: 17487773
    36. A/T mutagenesis in hypermutated immunoglobulin genes strongly depends on PCNAK164 modification PMID: 17664295
    37. Polkappa binds with monoubiquitinated proliferating cell nuclear antigen (PCNA) more robustly than with non-ubiquitinated PCNA. PMID: 18162470
    38. the SET8 and PCNA interaction couples H4-K20 methylation with DNA replication PMID: 18319261
    39. ubiquitylation of PCNA participates directly in the meiotic process and the diversification of the Ig locus through class-switch recombination (CSR) and somatic hypermutation (SHM) PMID: 18854411
    40. These data suggest that Prox1-PCNA interactions may in part prevent the activation of betaB1-crystallin expression in the lens epithelium. PMID: 19023449

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    PCNA family
  • 数据库链接：

    KEGG: mmu:18538

    STRING: 10090.ENSMUSP00000028817

    UniGene: Mm.391640