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  4. Recombinant Mouse Proteasome activator complex subunit 3 (Psme3)

Recombinant Mouse Proteasome activator complex subunit 3 (Psme3)

  • 中文名称：
    小鼠Psme3重组蛋白
  • 货号：
    CSB-YP018918MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Psme3重组蛋白
  • 货号：
    CSB-EP018918MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Psme3重组蛋白
  • 货号：
    CSB-EP018918MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Psme3重组蛋白
  • 货号：
    CSB-BP018918MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Psme3重组蛋白
  • 货号：
    CSB-MP018918MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Psme3; Proteasome activator complex subunit 3; 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-254
  • 氨基酸序列
    ASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.
  • 基因功能参考文献：
    1. the ubiquitin-independent REGgamma proteasome regulates energy homeostasis PMID: 27511885
    2. Study demonstrated that Psme3/Psme4 double KO (dKO) male mice are completely infertile and showed the up-regulation of several proteins involved in oxidative stress response in dKO sperms. These results suggest the important role of PA28gamma and PA200 in male fertility. PMID: 27003159
    3. Findings reveal a novel regulatory pathway in which the REGgamma-proteasome controls the steady-state level of GSK3beta protein. PMID: 26370326
    4. PSME3 is a target gene of NF-kappaB during bacterial infections. PSME3 activates NF-kappaB by degrading KLF2. PMID: 26776519
    5. REGgamma-mediated control of IkappaBvarepsilon contributes to NFkappaB activation and promotes bowel inflammation and associated tumour formation in response to chronic injury PMID: 26899380
    6. REGgamma acts in skin tumorigenesis mediating MAPK/p38 activation of the Wnt/beta-catenin pathway. PMID: 25908095
    7. In a p53-dominated cellular context, pro-apoptotic signaling might be overcome by PA28gamma-mediated caspase inhibition. PMID: 26201457
    8. Data suggest levels of gene expression of both Psme3 (proteasome activator subunit 3) and Dusp3 (dual specificity phosphatase 3) are associated with susceptibility to Staphylococcus aureus infection/sepsis in humans and in mouse disease model. PMID: 24901344
    9. REGgamma binds to SirT1, promotes its Ub-independent degradation, and inhibits its activity to deacetylate autophagy-related proteins, thereby inhibiting autophagy under normal conditions. PMID: 24011073
    10. REGgamma-deficient mice develop premature aging phenotypes that are associated with abnormal accumulation of casein kinase (CK)1delta and p53. Antibody array analysis identified CK1delta as a direct target of REGgamma. PMID: 23766372
    11. the regulation of REGgamma assembly and activity, suggesting a potential venue for the intervention of the ubiquitin-independent REGgamma proteasome activity. PMID: 23612972
    12. PA28gamma acts as a co-repressor of HTLV-1 p30 to suppress virus replication and is required for the maintenance of viral latency. PMID: 23104922
    13. PA28gamma stimulates MAFA degradation through a novel molecular mechanism that is distinct from that for the degradation of p21 PMID: 21830322
    14. Plasma cell differentiation to enable antibody secretion resulted in a transiently upregulated proteasome activator (PA) 28gamma, a component of the putative nuclear proteasome. PMID: 21319304
    15. REGgamma regulates cellular distribution of p53 by facilitating its multiple monoubiquitylation and nuclear export. PMID: 21084564
    16. REGgamma is present in many tissues and the highest expression is in the testis. PMID: 20494959
    17. Loss of PA28g expression in 28-kDA proteasome activator gamma-deficient mice results in antigen-specific defects in the processing and presentation of MHC class I-restricted T cell epitopes. PMID: 15004203
    18. REGgamma is not a viable therapeutic target in polyglutamine disease and that overall proteasome function is not impaired by trapped mutant polyglutamine in (Huntington's disease) R6/2 mice. PMID: 16311253
    19. PA28gamma plays a crucial role in the development of liver pathology induced by HCV infection PMID: 17234812

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
  • 蛋白家族：
    PA28 family
  • 数据库链接：

    KEGG: mmu:19192

    STRING: 10090.ENSMUSP00000019470

    UniGene: Mm.288477