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  4. Recombinant Mouse Protein Mdm4 (Mdm4)

Recombinant Mouse Protein Mdm4 (Mdm4)

  • 货号：
    CSB-YP013627MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013627MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013627MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013627MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013627MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mdm4; MdmxProtein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-489
  • 氨基酸序列
    MTSHSTSAQC SASDSACRIS SEQISQVRPK LQLLKILHAA GAQGEVFTMK EVMHYLGQYI MVKQLYDQQE QHMVYCGGDL LGDLLGCQSF SVKDPSPLYD MLRKNLVTSA SINTDAAQTL ALAQDHTMDF PSQDRLKHGA TEYSNPRKRT EEEDTHTLPT SRHKCRDSRA DEDLIEHLSQ DETSRLDLDF EEWDVAGLPW WFLGNLRNNC IPKSNGSTDL QTNQDIGTAI VSDTTDDLWF LNETVSEQLG VGIKVEAANS EQTSEVGKTS NKKTVEVGKD DDLEDSRSLS DDTDVELTSE DEWQCTECKK FNSPSKRYCF RCWALRKDWY SDCSKLTHSL STSNITAIPE KKDNEGIDVP DCRRTISAPV VRPKDGYLKE EKPRFDPCNS VGFLDLAHSS ESQEIISSAR EQTDIFSEQK AETESMEDFQ NVLKPCSLCE KRPRDGNIIH GKTSHLTTCF HCARRLKKSG ASCPACKKEI QLVIKVFIA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of TP53 activity than the long isoform.
  • 基因功能参考文献：
    1. MDMX is an important regulator of p53 DNA binding, which complements the role of MDM2 in regulating p53 level. PMID: 29581299
    2. The p53-null mice with the highest level of Mdm4 tended to have multiple tumours. Mdm4 transgenic mice in various genetic backgrounds shows synergy in tumour development in vivo. Mdm4 may thus serve as a therapeutic target in cancers. PMID: 27925213
    3. Data show that the Mdm4-p73 axis cannot override the dominant role of p53 in development and tumorigenesis and that Mdm4 and p73 interaction during development and tumorigenesis suggests new insight into the role of p53 family members. PMID: 26527653
    4. MDM4/HIPK2/p53 cytoplasmic assembly uncovers coordinated repression of molecules with anti-apoptotic activity during early DNA damage response. PMID: 25961923
    5. MDMx degradation associated with neuronal death occurs via caspase activation in neurons, and that the progressive loss of MDMx protein represents a potential mechanism of Abeta-induced neuronal death during disease progression in AD PMID: 26477779
    6. Increased Mdm4-S mRNA levels might correlate with more aggressive cancers without encoding significant amounts of a potential oncoprotein. PMID: 25088193
    7. Decreased Mdm4 levels improves the survival of mice expressing wild-type p53, but not that of mice expressing a p53 hypomorph lacking the proline-rich domain. PMID: 23474762
    8. identify Mdm4 as one of these key mRNAs that senses the defects in the spliceosomal machinery and transduces the signal to activate the p53 response PMID: 24013503
    9. The work demonstrates that the ability of MDM4 to enhance p53 stability is actually a specific property of MDM4 accomplished upon DNA damage. PMID: 22374672
    10. Mdm4 was depleted by infecteing with wtHAdV or a mutant virus lacking the E1B-55K gene (dl 1520). PMID: 22262167
    11. hypoxia can activate p53 through inactivation of MDMX by the ATR-Chk1-MDMX-14-3-3gamma pathway. PMID: 22556425
    12. a new role for 14-3-3gamma in protecting p21 from MDMX-mediated proteasomal turnover, which may partially account for DNA damage-induced elevation of p21 levels independent of p53. PMID: 21148311
    13. Mdm4 is thus a bona fide oncogene in vivo and cooperates with p53 heterozygosity to drive tumorigenesis. PMID: 20736370
    14. Murine mdmx gene also contains a functional p53-responsive P2 promoter in intron 1, and mdmx-P2 transcripts are clearly induced in response to diverse p53-activating signals. PMID: 20659896
    15. The death of mdm4-null embryos is due to p53-initiated cell cycle arrest . PMID: 14695178
    16. p21 is a downstream modifier of Mdm4, and provides genetic evidence that Mdm4 can function to regulate cell growth both positively and negatively. PMID: 14712235
    17. Mdmx overexpression allows primary mouse embryonic fibroblast immortalization and leads to neoplastic transformation in combination with HRas(V12). PMID: 15199139
    18. virtually all these data pointed toward a critical role for Mdmx in the regulation of the p53-Mdm2 network [review] PMID: 15865931
    19. Mdm4 cannot compensate for the loss of Mdm2 in heart development. PMID: 16354690
    20. MDM4 is alternatively spliced following UV irradiation. Alternate forms of MDM4 place selective pressure on the cells to acquire additional alterations in the p53 pathway. PMID: 17018606
    21. Mdm4 only contributes to p53 regulation at a specific phase of the differentiation program PMID: 17105817
    22. G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress. PMID: 17510313
    23. Activity of p53 was inhibited by Mdm4 even in the fully differentiated cardiomyocyte. Elevated apoptosis mediated by the p53 pathway in cardiomyocytes may be a mechanism for dilated cardiomyopathy. PMID: 17533180
    24. These data show that MDMX is an antiapoptotic factor in neurons, whose degradation is induced by various stresses and allows activation of p53 and E2F-1 during neuronal apoptosis. PMID: 17823617
    25. These results indicate that MdmX has a p53-independent role in suppressing oncogenic cell transformation, proliferation, and tumorigenesis by promoting centrosome clustering and bipolar mitosis. PMID: 18039860
    26. These data define a new localization and function of MDM4 that, by acting as a docking site for p53Ser46(P) to BCL2, facilitates the p53-mediated intrinsic-apoptotic pathway. PMID: 19521340
    27. Mdmx downregulation is crucial for effective p53-mediated radiation responses and tumor suppression in vivo in a mouse model of B cell lymphoma. PMID: 19573810

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    MDM2/MDM4 family
  • 组织特异性：
    In all tissues tested. The short isoform is expressed in a variety of transformed cell lines.
  • 数据库链接：

    KEGG: mmu:17248

    STRING: 10090.ENSMUSP00000070411

    UniGene: Mm.426531