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  4. Recombinant Mouse Protein VAC14 homolog (Vac14)

Recombinant Mouse Protein VAC14 homolog (Vac14)

  • 货号：
    CSB-YP802243MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP802243MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP802243MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP802243MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP802243MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Vac14
  • Uniprot No.：
  • 别名：
    Vac14; D8Wsu151e; Protein VAC14 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-782
  • 氨基酸序列
    MNPEKDFAPL TPNIVRALND KLYEKRKVAA LEIEKLVRDF VAQNNTMQIK HVIQTLSQEF ALSQHPHSRK GGLIGLAACS IALGKDSGLY LKELIEPVLT CFNDADSRLR YYACEALYNI VKVARGAVLP HFNVLFDGLS KLAADPDPNV KSGSELLDRL LKDIVTESSK FDLVSFIPLL RERIYSNNQY ARQFIISWIL VLVSVPDINL LDYLPEILDG LFQILGDNGK EIRKMCEVVL GEFLKEIKKN PSSVKFAEMA NILVIHCQTT DDLIQLTAMC WMREFIQLAG RVMLPYSSGI LTAVLPCLAY DDRKKSIKEV ANVCNQSLMK LVTPEDDEPD EPKSVAQKQT EPNPEDSLPK QEGTASGGPG SCDSSFGSGI NVFTSANTDR APVTLHLDGI VQVLNCHLSD TTIGMMTRIA VLKWLYHLYI KTPRKMFRHT DSLFPILLQT LSDESDEVVL KDLEVLAEIA SSPAGQTDDP GAPDGPDLRV NHSELQVPTS GRANLLNPPS TKGLEGSPST PTMNSYFYKF MINLLQTFSS ERKLLEARGP FIIRQLCLLL NAENIFHSMA DILLREEDLK FASTMVHTLN TILLTSTELF QLRNQLKDLQ TPESQNLFCC LYRSWCHNPV TTVSLCFLTQ NYRHAYDLIQ KFGDLEVTVD FLTEVDKLVQ LIECPIFTYL RLQLLDVKNN PYLIKALYGL LMLLPQSSAF QLLSHRLQCV PNPELLQTED CLKAAPKSQK GDSPSIDYTE LLQHFEKVQK QHLEVRHQRS GRGDHLDRRV IL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped structure and nucleates the assembly of the complex. The pentamer binds a single copy each of PIKFYVE and FIG4 and coordinates both PIKfyve kinase activity and FIG4 phosphatase activity, being required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
  • 基因功能参考文献：
    1. Thus, VAC14, PI(3,5)P(2) and/or PI(5)P play a role in controlling postsynaptic function via regulation of endocytic cycling of AMPA receptors. PMID: 22842785
    2. The data demonstrate for the first time a physical association between functionally related PIKfyve and ArPIKfyve in 3T3-L1 adipocytes. PMID: 17475247
    3. report the characterization of a mouse mutant lacking Vac14, a regulator of PI(3,5)P(2) synthesis PMID: 17956977
    4. The s show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18. PMID: 19037259
    5. This publication pertains to studies in yeast. PMID: 11889142

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  • 相关疾病：
    Defects in Vac14 are the cause of the infantile gliosis phenotype (ingls). Mice exhibit reduced body size and diluted pigmentation that can be recognized as early as postnatal day 3 (P3). By P14, the mice exhibit a tremor and impaired motor function. Maximal survival of the mice is for 3 weeks. Small areas with the appearance of spongiform degeneration are visible in several brain regions, including the thalamus, brain stem and cerebellar nucleus.
  • 亚细胞定位：
    Endosome membrane. Microsome membrane.
  • 蛋白家族：
    VAC14 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:234729

    STRING: 10090.ENSMUSP00000034190

    UniGene: Mm.274894