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  4. Recombinant Mouse Protein artemis (Dclre1c)

Recombinant Mouse Protein artemis (Dclre1c)

  • 货号：
    CSB-YP817008MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP817008MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP817008MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP817008MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP817008MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dclre1c
  • Uniprot No.：
  • 别名：
    Dclre1c; Art; Snm1lProtein artemis; mArt; EC 3.1.-.-; DNA cross-link repair 1C protein; SNM1-like protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-705
  • 氨基酸序列
    MSSFQGQMAE YPTISIDRFD RENLKARAYF LSHCHKDHMK GLRAPSLKRR LECSLKVFLY CSPVTKELLL TSPKYRFWEN RIITIEIETP TQISLVDEAS GEKEEVVVTL LPAGHCPGSV MFLFQGSNGT VLYTGDFRLA KGEASRMELL HSGGRVKDIQ SVYLDTTFCD PRFYQIPSRE QCLRGILELV RSWVTRSPHH VVWLNCKAAY GYEYLFTNLS EELGVQVHVD KLDMFKNMPD ILHHLTTDRN TQIHACRHPK AEECFQWNKL PCGITSQNKT ALHTISIKPS TMWFGERTRK TNVIVRTGES SYRACFSFHS SFSEIKDFLS YICPVNVYPN VIPVGLTVDK VMDVLKPLCR SPQSVEPKYK PLGKLKRART IHLDSEEDDD LFDDPLPTPL RHKVPYQLTL QPELFSMKAL PLDQPELRQS PGGCKAESVW SPSLANFIDC EESNSDSGEE LETPPPSLQG GLGPSTLVQQ NADPDVDIPQ WEVFFKRRDE ITGECLEHLP SSIETGGSQS PKLCSDSPKL CSDSPKLCSD SDGDSTHISS QNSSQSTHIT DQGSQGWDSQ CDTVLLSSQE KSGGDSTSLN KGAYKPKLKE SISASQIEQD ALCPQDTHCD LKSRAEVNGA PCLVELDTLS GRKSPPEKTL LSSTRADSQS SSDFEIPSTP EAELPTPEHL QCLYRKLATG QSIVVEKRKC SLLDS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein likely exhibits single-strand specific 5'-3' exonuclease activity in isolation, and may acquire endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity may be required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
  • 基因功能参考文献：
    1. ATM phosphorylates DNA-PKcs to recruit Artemis and promote end-processing. PMID: 25818648
    2. we present T(-)B(-)NK(+) severe combined immunodeficiency (SCID) phenotype after spontaneously occurring modification of Artemis gene in mice. PMID: 24069355
    3. deficient mice have a phenotype similar to that of DNA-PKcs-deficient mice-including severe combined immunodeficiency associated with defects in opening and joining V(D)J coding hairpin ends and increased cellular ionizing radiation sensitivity (artemis) PMID: 12504013
    4. Data show that Artemis appears to be required for a subset of nonhomologous DNA end joining reactions that require end processing [Artemis]. PMID: 12615897
    5. Artemis/p53-deficient mouse tumors lacked der(12)t(12;15) translocations and c-myc amplification. PMID: 14983023
    6. V(D)J and DNA repair defects seen in this Artemis-deficient mouse model are direct evidence that defective Artemis is the pathologic mechanism for the immunodeficiency phenotype of SCID in Athabascan-speaking Native Americans. PMID: 15699179
    7. DNA-PK has Artemis-independent functions in class switch recombination and normal development PMID: 15699324
    8. Since Art deficient mice represent a model for radiation-sensitive severe combined immunodeficiency, we suggest that these patients may be at risk for both lymphoid and non-lymphoid cancers. PMID: 17384673
    9. DNA-PKcs and Artemis open AAV inverted terminal repeat (ITR) hairpin loops in a tissue-dependent manner. PMID: 17686847
    10. both DNA-PKcs and, unexpectedly, Artemis are necessary for joining a subset of activation-induced cytidine deaminase (AID)-dependent DNA double-strand breaks PMID: 18316419
    11. These data suggest that Artemis might be the nuclease responsible for nucleotide loss from signal ends during the repair process. PMID: 18501428
    12. Loss of Artemis leads to reduced immunoglobulin class switch recombination. PMID: 19692705

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA repair metallo-beta-lactamase (DRMBL) family
  • 数据库链接：

    KEGG: mmu:227525

    STRING: 10090.ENSMUSP00000100053

    UniGene: Mm.23483