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  4. Recombinant Mouse Protein cereblon (Crbn)

Recombinant Mouse Protein cereblon (Crbn)

  • 货号：
    CSB-EP806030MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806030MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806030MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806030MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Name:Crbn
  • Uniprot No.：
  • 别名：
    CrbnProtein cereblon; Protein PiL
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-445
  • 氨基酸序列
    MAGEGDQQDA AHNMGNHLPL LPADSEDEDD EIEMEVEDQD SKEARKPNII NFDTSLPTSH TYLGADMEEF HGRTLHDDDS CQVIPVLPEV LMILIPGQTL PLQLSHPQEV SMVRNLIQKD RTFAVLAYSN VQEREAQFGT TAEIYAYREE QEFGIEVVKV KAIGRQRFKV LELRTQSDGI QQAKVQILPE CVLPSTMSAV QLESLNKCQV FPSKPISWED QYSCKWWQKY QKRKFHCANL TSWPRWLYSL YDAETLMDRI KKQLREWDEN LKDDSLPENP IDFSYRVAAC LPIDDVLRIQ LLKIGSAIQR LRCELDIMNK CTSLCCKQCQ ETEITTKNEI FSLSLCGPMA AYVNPHGYVH ETLTVYKASN LNLIGRPSTV HSWFPGYAWT IAQCKICASH IGWKFTATKK DMSPQKFWGL TRSALLPTIP ETEDEISPDK VILCL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently, cognitive functions such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism.
  • 基因功能参考文献：
    1. Collectively, our data suggested that CRBN is a potential treatment option against diabetes-induced osteolytic bone disease. PMID: 29353038
    2. Data show that cereblon (CRBN) limits CD4(+) T-cell activation via epigenetic regulation of Kv1.3 potassium channel (Kv1.3) expression. PMID: 27439875
    3. CRBN negatively regulates TLR4 signaling via attenuation of TRAF6 and TAB2 ubiquitination. PMID: 27468689
    4. CRBN is involved in the activation of PERK and the Ire1 pathway of the unfolded protein response.CRBN plays a role in cellular stress signaling, including the unfolded protein response, by controlling the activity of AMPK. PMID: 25619137
    5. results of this study suggest a plausible mechanism for CRBN involvement in higher brain function in humans, and they may help explain how a specific mutation in CRBN can affect the cognitive ability of patients PMID: 24993823
    6. Found significantly smaller infarct sizes and less fibrosis in the hearts of CRBN KO mice than in those of WT mice. Apoptosis was also significantly reduced in the KO mice compared with that in WT mice. PMID: 24755080
    7. findings suggest that crbn-/- mice exhibit selective HPC-dependent deficits in associative learning. PMID: 21995942
    8. CRBN directly interacts with the alpha1 subunit of AMP-activated protein kinase (AMPK alpha1) and inhibits the activation of AMPK activation. PMID: 21232561
    9. These results imply that Nrf2 stimulates CRBN gene transcription under hypoxia-reoxygenation conditions in neuronal cells. PMID: 20691658

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    CRBN family
  • 组织特异性：
    Highly expressed in brain.
  • 数据库链接：

    KEGG: mmu:58799

    STRING: 10090.ENSMUSP00000061604

    UniGene: Mm.290085