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  4. Recombinant Mouse Protein-cysteine N-palmitoyltransferase porcupine (Porcn), partial

Recombinant Mouse Protein-cysteine N-palmitoyltransferase porcupine (Porcn), partial

  • 中文名称：
    小鼠Porcn重组蛋白
  • 货号：
    CSB-YP884929MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Porcn重组蛋白
  • 货号：
    CSB-EP884929MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Porcn重组蛋白
  • 货号：
    CSB-EP884929MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Porcn重组蛋白
  • 货号：
    CSB-BP884929MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Porcn重组蛋白
  • 货号：
    CSB-MP884929MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Porcn; Porc; Ppn; Protein-serine O-palmitoleoyltransferase porcupine; mPORC; EC 2.3.1.250
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of WNT proteins is required for efficient binding to frizzled receptors.
  • 基因功能参考文献：
    1. Porcn mosaicism results in reduced fertility. PMID: 29066530
    2. This study showed that postnatal ablation of Porcupine in the uterine luminal epithelium alone results in the decrease in endometrial gland number; mutant females are completely infertile. PMID: 29036275
    3. Data suggest that PORCN exhibits substrate specificity that includes a Wnt3a peptide fragment (residues 199-219, with disulfide bonds); recombinant PORCN containing a point mutation (R228C) associated with focal dermal hypoplasia exhibits impaired acylation activity toward Wnt3a peptide fragment. (PORCN = porcupine O-acyltransferase; Wnt3a = Wnt family member 3A) PMID: 28655768
    4. Porcupine-dependent Wnt signaling, unlike previously reported, is dispensable for postnatal gland formation but is required for post-pubertal gland maintenance as well as for stromal cell proliferation. PMID: 27965056
    5. Overexpression of PORCN enhances the ability of WLS to promote WNT1 trafficking to the cell surface as well as secretion, but decreases the ability of WLS to activate WNT1 signaling in target cell. PMID: 27492485
    6. Inhibition of porcupine abrogates kidney fibrosis not only by overcoming the redundancy of individual Wnt isoforms but also by preventing upstream cytokine-induced Wnt generation. PMID: 27083283
    7. Conditional PORCN knockout mice exhibit specific changes in AMPAR expression and gating that reduce basal synaptic transmission but leave long-term potentiation intact. PMID: 26776514
    8. Porcn is required in both extraocular and neuroectodermal tissues to regulate distinct Wnt-dependent processes during morphogenesis of the posterior and anterior segments of the eye PMID: 25451153
    9. Residues in predicted transmembrane domain 9 within Porcupine are essential for palmitoleoylation. PMID: 24798332
    10. Data show bronchopneumonia, rhinitis, and otitis media, suggesting a potential link between Porcn function and the normal development of ciliated cells. PMID: 24223895
    11. Studies highlight the importance of Wnt3 and Wnt7b for embryonic and placental development but suggest that endogenous Porcn-dependent Wnt secretion does not play an essential role in either implantation or blastocyst lineage specification. PMID: 23760955
    12. an essential regulatory role of PORCN in shaping Wnt signaling gradients. PMID: 22888000
    13. Porcn inactivation in the mouse produces a model for human FDH and that phenotypic features result from defective WNT signaling in ectodermal- and mesenchymal-derived structures. PMID: 22412863
    14. Porcupine homolog is required for canonical Wnt signaling and gastrulation in mouse embryos. PMID: 21554866

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Membrane-bound acyltransferase family, Porcupine subfamily
  • 组织特异性：
    Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis. Isoform 4 is strongly expressed in kidney, liver, lung, spleen and testis. Isoform 1 is strongly expressed in brain, heart and muscle and poorly in kidney, liver, lung, spleen and
  • 数据库链接：

    KEGG: mmu:53627

    STRING: 10090.ENSMUSP00000076790

    UniGene: Mm.443425