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  4. Recombinant Mouse Protein-glutamine gamma-glutamyltransferase 2 (Tgm2)

Recombinant Mouse Protein-glutamine gamma-glutamyltransferase 2 (Tgm2)

  • 货号：
    CSB-YP023462MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023462MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023462MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023462MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023462MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tgm2
  • Uniprot No.：
  • 别名：
    Tgm2; Protein-glutamine gamma-glutamyltransferase 2; EC 2.3.2.13; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase-2; TGase-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-686
  • 氨基酸序列
    AEELLLERC DLEIQANGRD HHTADLCQEK LVLRRGQRFR LTLYFEGRGY EASVDSLTFG AVTGPDPSEE AGTKARFSLS DNVEEGSWSA SVLDQQDNVL SLQLCTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLYN AWCPADDVYL DSEEERREYV LTQQGFIYQG SVKFIKSVPW NFGQFEDGIL DTCLMLLDMN PKFLKNRSRD CSRRSSPIYV GRVVSAMVNC NDDQGVLLGR WDNNYGDGIS PMAWIGSVDI LRRWKEHGCQ QVKYGQCWVF AAVACTVLRC LGIPTRVVTN YNSAHDQNSN LLIEYFRNEF GELESNKSEM IWNFHCWVES WMTRPDLQPG YEGWQAIDPT PQEKSEGTYC CGPVSVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIRQEDG SVLKSINRSL VVGQKISTKS VGRDDREDIT HTYKYPEGSP EEREVFTKAN HLNKLAEKEE TGVAMRIRVG DSMSMGNDFD VFAHIGNDTS ETRECRLLLC ARTVSYNGVL GPECGTEDIN LTLDPYSENS IPLRILYEKY SGCLTESNLI KVRGLLIEPA ANSYLLAERD LYLENPEIKI RVLGEPKQNR KLVAEVSLKN PLSDPLYDCI FTVEGAGLTK EQKSVEVSDP VPAGDLVKAR VDLFPTDIGL HKLVVNFQCD KLKSVKGYRN VIIGPA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono- and polyamines, thereby producing cross-linked or aminated proteins, respectively. Involved in many biological processes, such as bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification and apoptosis. Acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of proteins, such as ACO2, HSPB6, FN1, HMGB1, RAP1GDS1, SLC25A4/ANT1, SPP1 and WDR54. Under physiological conditions, the protein cross-linking activity is inhibited by GTP; inhibition is relieved by Ca(2+) in response to various stresses. When secreted, catalyzes cross-linking of proteins of the extracellular matrix, such as FN1 and SPP1 resulting in the formation of scaffolds. Plays a key role during apoptosis, both by (1) promoting the cross-linking of cytoskeletal proteins resulting in condensation of the cytoplasm, and by (2) mediating cross-linking proteins of the extracellular matrix, resulting in the irreversible formation of scaffolds that stabilize the integrity of the dying cells before their clearance by phagocytosis, thereby preventing the leakage of harmful intracellular components. In addition to protein cross-linking, can use different monoamine substrates to catalyze a vast array of protein post-translational modifications: mediates aminylation of serotonin, dopamine, noradrenaline or histamine into glutamine residues of target proteins to generate protein serotonylation, dopaminylation, noradrenalinylation or histaminylation, respectively. Mediates protein serotonylation of small GTPases during activation and aggregation of platelets, leading to constitutive activation of these GTPases. Plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3. Catalyzes serotonylation of 'Gln-5' of histone H3 (H3Q5ser) during serotonergic neuron differentiation, thereby facilitating transcription. Acts as a mediator of neurotransmission-independent role of nuclear dopamine in ventral tegmental area (VTA) neurons: catalyzes dopaminylation of 'Gln-5' of histone H3 (H3Q5dop), thereby regulating relapse-related transcriptional plasticity in the reward system. Regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2. Also acts as a protein deamidase by mediating the side chain deamidation of specific glutamine residues of proteins to glutamate. Catalyzes specific deamidation of protein gliadin, a component of wheat gluten in the diet. May also act as an isopeptidase cleaving the previously formed cross-links. Also able to participate in signaling pathways independently of its acyltransferase activity: acts as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism.
  • 基因功能参考文献：
    1. TG2 activity enhanced p65 phosphorylation, leading to an increase in NF-kappaB transcriptional activity. These results indicate that TG2 is a critical mediator of cytokine expression in the UV-induced inflammatory response of keratinocytes. PMID: 29072680
    2. Following pressure overload, endogenous tTG mediates matrix cross-linking, while protecting the remodelling myocardium from dilation by exerting matrix-preserving actions PMID: 28371893
    3. that TG2 depletion increases nuclear factor-kappaB (NF-kappaB) signaling PMID: 28522262
    4. Factor XIII-A and TG2 regulate resorption, adipogenesis and plasma fibronectin homeostasis in bone and bone marrow. PMID: 28387755
    5. tTG might be a key factor in pathogenesis of abnormal protein aggregation in Alzheimer's disease. PMID: 26386840
    6. data suggest that TG2 has a tissue-specific role in GONAT function and browning, which becomes apparent under acute cold exposure. PMID: 28774822
    7. Adenosine produced from adenine nucleotides through an interaction between apoptotic cells and engulfing macrophages contributes to the appearance of TGM2 in dying thymocytes. PMID: 27236567
    8. TG2 is expressed in infiltrating and adhering to spinal cord monocytes during experimental autoimmune encephalomyelitis in transgenic mice. PMID: 27826792
    9. These results indicate that TG2-mediated Th17 cell differentiation is not required for the pathogenesis of dextran sulfate sodium-induced acute colitis. PMID: 27811936
    10. The increased expression of TGM2 in the TM increases N-epsilon(gamma-glutamyl) lysine crosslinking in the TM, increases aqueous outflow resistance, and elevates IOP in mice. TGM2 may be at least partially responsible for ocular hypertension in POAG. PMID: 29222550
    11. Two enhancers of Tgm2, which seem to act as integrators of the TGF-beta, retinoid and adenylate cyclase signaling pathways in dying thymocytes have been identified. PMID: 27262403
    12. TG2 transamidating activity is induced upon proteasome inhibition. Proteasome inhibition leads to the selective recruitment of TG2 in the exosomes. Exosomes from cells lacking TG2 present less protein content. TG2 interacts with ESCRT proteins upon stressful stimulus. TG2 is an important player in the biogenesis of exosomes controlling the selectivity of their cargo under stressful cellular conditions. PMID: 27169926
    13. data suggest that the anti-angiogenic mechanism of the celiac disease-specific autoantibodies involves extracellular TG2 and inhibited endothelial cell mobility PMID: 23824706
    14. Kidney fibrosis in aging may represent a natural outcome of upregulated endostatin (EST) and transglutaminase 2 (TG2), but more likely it appears to be a result of cumulative stresses occurring on the background of synergistically acting geronic (aging) proteins, EST and TG2. PMID: 27165830
    15. findings support the potential pathophysiological relevance of TRX in celiac disease and establish the Cys(370)-Cys(371) disulfide bond of TG2 as one of clearest examples of an allosteric disulfide bond in mammals. PMID: 28003361
    16. TG2 controls the formation of VEGF165-heparan sulfate proteoglycan complexes PMID: 26175493
    17. TG2 is a novel inhibitor of adipogenesis. PMID: 26313919
    18. LPS-induced TG2 was involved in the mechanism of pinocytosis and phagocytosis in microglia. PMID: 25301694
    19. Inhibition of tissue transglutaminase resulted in a reduced rate of compaction compared to controls during early remodeling (up to 2 days). In contrast, inhibition of lysyl oxidase did not alter the early compaction. PMID: 25924936
    20. TG2-deficient mice exhibited hyper inflammatory responses after being challenged with monosodium urate crystals. PMID: 25889736
    21. Data presented in this study show that TG2 plays a key role in cellular dynamics and consequently influences the energetic metabolism. PMID: 25060553
    22. TG2 functions as a critical signal for allergic lung responses. PMID: 26209276
    23. TG2 ablation had no effect on either motor or cognitive deficits, or on the weight loss. PMID: 24955833
    24. TG2 is an important mediator of endothelial cell inflammation and lung PMN sequestration associated with intravascular coagulation and sepsis PMID: 25057925
    25. TG2 promotes neointima formation by mediating the PDGF-BB activation of the PDGFR/Akt1 and beta-catenin pathways in vascular smooth muscle cells. PMID: 25612735
    26. adrenoceptor-mediated increase in macrophage TG2 expression might be associated with stress-related inflammatory disorders. PMID: 24863408
    27. miR-218 and miR-181a formed a negative feedback loop with TGaseII and regulated the in vitro and in vivo allergic inflammation. PMID: 25202021
    28. Studies indicate that cells undergoing apoptosis show an increased level of transglutaminase 2 (TG2) expression. PMID: 24778599
    29. The results indicate possible roles of TG2 in CD8(+) T-cell activation and CD8(+) memory T-cell generation. PMID: 24628083
    30. Pulmonary TG2 undergoes marked post-translational activation in a mouse model of hypoxia-induced pulmonary hypertension. PMID: 24152195
    31. Syndecan-4 is required for cell surface targeting of TG2 and the development of kidney fibrosis in chronic kidney disease. PMID: 24357671
    32. TG2 on the surface of dendritic cells modulates the dendritic cell-T cell interaction. PMID: 24727157
    33. Data suggest binding of autoantibodies to TGM2 in vascular endothelium of endometrium represent key mechanism by which anti-TGM2 antibodies alters embryo implantation/placentation in celiac disease; study in nude mice provided confirmatory results. PMID: 23966323
    34. tTG has an intrinsic capability to stimulate cell survival through a novel mechanism that activates PI3-kinase signaling events PMID: 24569994
    35. Role for TG2 in GPR56-mediated melanoma inhibition. The uncovered antagonistic relationship between GPR56 and TG2 proposes a mechanism by which extracellular matrix accumulation/crosslinking in tumors may be reversed. PMID: 24356421
    36. No significant differences between WT and TG2(R579A/R579A) mice. PMID: 23717413
    37. TG2 inhibition results in inhibition of fibronectin deposition in human umbilical vein endothelial cell monocultures with a parallel reduction in matrix-bound VEGFA. PMID: 24052076
    38. We identify a novel pathogenic involvement of TG2 in preeclampsia but also suggest a previously unrecognized role of TG2 in the regulation of G protein-coupled receptor stabilization by inhibiting ubiquitination-dependent degradation. PMID: 24191290
    39. These results suggest that Sp1 cross-linking might be crucial in negative regulation of PAC1 gene expression due to TG2 in OGD-induced ER stress. PMID: 24045949
    40. Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin. PMID: 24036114
    41. Thus eNOS-derived NO regulates TG2 localization and function and contributes to vascular stiffness. PMID: 23873798
    42. Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma. PMID: 23704086
    43. TGase 2 expression and Ca2+ influx required by bidirectional events in mast cell activation had an important role in IgE production in B cells via CD40/CD40L interaction. PMID: 23524335
    44. TG2-deficient mice showed reduced IL-33 expression following induction of allergic asthma compared to those in the WT control. PMID: 23496815
    45. Loss of TG2 is associated with reduced corneal wound closure. PMID: 23466867
    46. Investigated the effect of TG2 phosphorylation on NF-kappaB, Akt and PTEN using embryonic fibroblasts derived from TG2 null mice (MEF(tg2-/-)) overexpressing native TG2 or mutant-TG2 (m-TG2) lacking Ser(216) phosphorylation site. PMID: 22759359
    47. TGase 2 may regulate the balance between cell survival and cell death through the modulation of CTSD levels. PMID: 21960143
    48. TGase 2 differently participated in increased hemodynamic shock in LPS-induced septic shock through macrophage activation rather than protecting against direct liver damage. PMID: 23053022
    49. Physiological significance of mouse TG2 and Factor XIII through identification of substrate proteins in MC3T3-E1 cells. PMID: 22045386
    50. Tissue-specific mechanisms of compensation for the loss of TG2, including transcriptional compensation. PMID: 22194042

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  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Chromosome. Secreted, extracellular space, extracellular matrix. Cell membrane. Mitochondrion.
  • 蛋白家族：
    Transglutaminase superfamily, Transglutaminase family
  • 数据库链接：

    KEGG: mmu:21817

    STRING: 10090.ENSMUSP00000099411

    UniGene: Mm.330731