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  4. Recombinant Mouse Protein kinase C delta type (Prkcd)

Recombinant Mouse Protein kinase C delta type (Prkcd)

  • 货号：
    CSB-YP018702MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018702MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018702MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018702MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018702MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkcd; PkcdProtein kinase C delta type; EC 2.7.11.13; Tyrosine-protein kinase PRKCD; EC 2.7.10.2; nPKC-delta) [Cleaved into: Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit; Sphingosine-dependent protein kinase-1; SDK1)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-674
  • 氨基酸序列
    MAPFLRISFN SYELGSLQVE DEASQPFCAV KMKEALSTER GKTLVQKKPT MYPEWKTTFD AHIYEGRVIQ IVLMRAAEDP VSEVTVGVSV LAERCKKNNG KAEFWLDLQP QAKVLMCVQY FLEDGDCKQS MRSEEEAKFP TMNRRGAIKQ AKIHYIKNHE FIATFFGQPT FCSVCKEFVW GLNKQGYKCR QCNAAIHKKC IDKIIGRCTG TATNSRDTIF QKERFNIDMP HRFKVYNYMS PTFCDHCGSL LWGLVKQGLK CEDCGMNVHH KCREKVANLC GINQKLLAEA LNQVTQRSSR KLDTTESVGI YQGFEKKPEV SGSDILDNNG TYGKIWEGST RCTLENFTFQ KVLGKGSFGK VLLAELKGKD KYFAIKCLKK DVVLIDDDVE CTMVEKRVLA LAWESPFLTH LICTFQTKDH LFFVMEFLNG GDLMFHIQDK GRFELYRATF YAAEIICGLQ FLHSKGIIYR DLKLDNVMLD RDGHIKIADF GMCKENIFGE GRASTFCGTP DYIAPEILQG LKYSFSVDWW SFGVLLYEML IGQSPFHGDD EDELFESIRV DTPHYPRWIT KESKDIMEKL FERDPDKRLG VTGNIRIHPF FKTINWSLLE KRKVEPPFKP KVKSPSDYSN FDPEFLNEKP QLSFSDKNLI DSMDQEAFHG FSFVNPKFEQ FLDI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. Involved in antifungal immunity by mediating phosphorylation and activation of CARD9 downstream of C-type lectin receptors activation, promoting interaction between CARD9 and BCL10, followed by activation of NF-kappa-B and MAP kinase p38 pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion. Phosphorylates ELAVL1 in response to angiotensin-2 treatment. Phosphorylates mitochondrial phospolipid scramblase 3 (PLSCR3), resulting in increased cardiolipin expression on the mitochondrial outer membrane which facilitates apoptosis. Phosphorylates SMPD1 which induces SMPD1 secretion.
  • 基因功能参考文献：
    1. this study shows that PKCdelta is important for optimal macrophage killing effector functions and survival in mice with tuberculosis PMID: 28832027
    2. Our key findings provide novel insights into the mechanism of action of heterotrimeric complex (PKCdelta-TIRAP-p38) in proinflammatory cytokine expression, which controls the development of the inflammatory trigger in stimulated macrophages. PMID: 28528205
    3. sustained PKCdelta activation drives sustained microglial inflammatory responses and concomitant dopaminergic neurotoxicity consequently leading to neurobehavioral deficits PMID: 27151770
    4. spinal PKCdelta has a role in the development of chronic pain in SCD, which may become a potential target for pharmacological interventions PMID: 27348590
    5. MALAT1 recruits splice factor serine-arginine-rich splice factor 2 (SRSF2) to promote alternative splicing of PKCdeltaII. PMID: 27841943
    6. SphK1 dependent PKC-delta activation plays an important role in promoting NF-kappaB activation and inflammatory response in acute liver failure, and inhibition of PKC-delta activation might be a potential therapeutic strategy for this disease. PMID: 26730154
    7. PKCdelta activity exerts a dual role through the autophagy mechanism, decreasing proliferative capacity of mammary tumor cells but also regulating tumor stem cell self-renewal. PMID: 26335446
    8. alpha(1)-AR activation is anti-apoptotic and protective during cardiac ischemia due to glucose deprivation and not hypoxia by enhancing glucose uptake into the heart via PKCdelta-mediated GLUT translocation that may be specific to the alpha(1A)-AR subtype. PMID: 26832303
    9. Study shows that PRKCD influences corticotroph cell viability, POMC and ACTH expression suggesting that PRKCD plays an important role in restraining corticotroph cell proliferation and function. PMID: 26522132
    10. PKCdelta-mediated phosphorylation of 14-3-3zeta contributes to the nuclear retention of FOXO1, even when FOXO1 is phosphorylated as under non-stress conditions PMID: 26363783
    11. The homozygous Prkcqrpea1 and the null Prkcqtm1Litt mutants are reliable novel mouse models of RD and can also be used to study the effects of the disruption of PRKCQ (PKCtheta;) signaling in RPE cells. PMID: 26978024
    12. function of protein kinase C delta (PKCdelta) during fertilization and embryonic development PMID: 26202826
    13. A novel PKCdelta signaling pathway that requires retinol as a metabolic cofactor. PMID: 26671999
    14. Data suggest that cytokines (TNF-alpha, IL1-beta, IFN-gamma) disrupt gap junction coupling in pancreatic islets under conditions associated with prediabetes; the mechanism appears to involve nitric oxide-mediated activation of protein kinase C-delta. PMID: 26668311
    15. PKCdelta acts as a proapototic kinase in oxidized phospholipid-included apoptosis of RAW 264.7 macrophages. PMID: 26707247
    16. Data show that protein kinase C delta (PKC-delta) knockout mice display atypical structural alterations at the articular cartilage and bone-cartilage interface. PMID: 26279273
    17. Data (including data from studies in transgenic/knockout mice) suggest expression of PKCd in skeletal muscle is up-regulated in middle-aged mice; muscle-specific deletion of PKCd improves middle-age related glucose intolerance and insulin resistance. PMID: 26307588
    18. PKCdelta and PKCepsilon have roles in restraining developmental and reactive heart growth PMID: 25900833
    19. Pathway analysis indicated that monosaccharide metabolism was affected only upon PKCdelta deletion, while isoprenoid biosynthesis was affected in a PKCepsilon-specific manner. PMID: 25175814
    20. This study demonstrate that both the tyrosine kinase Fyn and the serine/threonine kinase PKCdelta are differentially expressed in microglia and astrocytes. PMID: 26157004
    21. the circadian cycle cannot be a key factor in modulating methamphetamine toxicity under the current experimental condition and that PKCdelta is one of the critical target genes for melatonin-mediated protective effects against mitochondrial burdens PMID: 25407782
    22. The development of anti-dsDNA autoantibodies and glomerulonephritis with IgG deposition in mice expressing a doxycycline-induced dominant negative PKCdelta (dnPKCdelta) in T cells is reported. PMID: 25829232
    23. data suggest a redundant role between members of the novel Protein Kinase C subfamily that allows for mutual compensation during mouse embryonic development, with vasculogenesis/angiogenesis as an obvious common function of these two Protein Kinase Cs. PMID: 25084151
    24. deficiency of PKCdelta caused an increase in white blood cells and platelet counts, as well as in bone marrow and splenic megakaryocytes (P<0.05) PMID: 25359855
    25. analysis of ATP analog-specific protein kinase Cdelta PMID: 25505183
    26. Our findings reveal the following novel molecular mechanisms controlling mitochondrial fission during A/R injury of cardiomyocytes resulted from both increased activation Drp1 through Cdk1, PKCdelta, and calcineurin-mediated pathways, respectively PMID: 25445585
    27. histone acetylation regulates PKCdelta expression to augment nigrostriatal dopaminergic cell death, which could contribute to the progressive neuropathogenesis of Parkinson disease PMID: 25342743
    28. TRA2B has a role in regulating protein kinase C deltaI splice variant expression during 3T3L1 preadipocyte cell cycle PMID: 25261467
    29. Protein kinase C-delta mediates sepsis-induced activation of complement 5a and urokinase-type plasminogen activator signaling in macrophages PMID: 24682410
    30. Over-expression of PKCdelta increases mutant huntingtin-induced cell death in vitro, whereas over-expression of a PKCdelta dominant negative form or silencing of endogenous PKCdelta partially blocks mutant huntingtin-induced cell death. PMID: 23896721
    31. PKCdelta may be involved in the development of obesity-associated NAFLD by regulating hepatic lipid metabolism and insulin signaling PMID: 25035929
    32. PKC delta plays a role in key pathological features of fatty liver disease but not ultimately in fibrosis in the mouse model of nonalcoholic steatohepatitis. PMID: 24454937
    33. PKCdelta is likely to contribute to phenylephrine-induced contraction in obese mouse aortae. PMID: 24667603
    34. Membrane tethering of PKCdelta leads to increased force exertion on extracellular matrix through PLCgamma1/EGFR signaling. PMID: 24155954
    35. protein kinase Cdelta inhibition protects the salivary gland from radiation damage PMID: 24569990
    36. PKCdelta deficiency leads to the survival and development of a B cell repertoire that is not only aberrantly autoreactive but also hyperresponsive to antigen stimulation PMID: 24515435
    37. PKCdelta activates NF-kappaB through an IkappaB-independent cytosolic interaction PMID: 24519937
    38. PKCdelta deficient mice exhibit increased trabeculae bone containing residual cartilage matrix, indicative of an osteoclast-rich osteopetrosis phenotype. PMID: 23951014
    39. Notch and PKCdelta are involved in formation of the lateral region of the dorso-ventral axis in Drosophila embryos. PMID: 23861806
    40. analysis of how protein kinase C delta (PKCdelta) splice variants modulate the apoptosis pathway in 3T3L1 cells during adipogenesis PMID: 23902767
    41. The regulation of runt-related transcription factor 2 by fibroblast growth factor-2 and connexin43 requires the inositol polyphosphate/protein kinase Cdelta cascade. PMID: 23322705
    42. PKCdelta impaired vessel formation and angiogenic factor expression in diabetic ischemic limbs PMID: 23557702
    43. analysis of a GAPDH/PKCdelta signaling switch, which is activated during oxidative stress to regulate the balance between cell survival by mitophagy and cell death due to accumulation of damaged mitochondria PMID: 23653351
    44. These results indicate that LPA-stimulated PKD2 phosphorylation requires PKCdelta and non-catalytic actions of PI3K p110beta, and provide new information with respect to GPCR-mediated signal transduction in myoblasts. PMID: 23252604
    45. strong expression of PKCdelta in ganglia during murine development shown in this study suggests a significant role of this isoform as well as redundancy with other PKCs within the nervous system, since PKCdelta deficient mice develop normally PMID: 23305608
    46. Proteolytic activation of protein kinase Cdelta is a key downstream effector of dopaminergic cell death. PMID: 22540228
    47. PKCdelta plays a critical role in diabetes-induced neural tube defects, potentially through increasing oxidative and ER stress and JNK-associated stress-response pathways. PMID: 22463764
    48. PKCdelta deficiency does not perturb formation of the ruffled border or trafficking of lysosomal vesicles containing the vacuolar-ATPase (v-ATPase). PMID: 22806935
    49. PKCdelta plays a role in the pathological progression of endotoxin-induced lung injury, likely mediated through modulation of inflammatory signaling and pulmonary vascular barrier function. PMID: 22983354
    50. Elevated protein kinase C-delta contributes to aneurysm pathogenesis through stimulation of apoptosis and inflammatory signaling. PMID: 22879584

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell membrane; Peripheral membrane protein. Mitochondrion. Endomembrane system.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 组织特异性：
    Isoform 1 is highly expressed in developing pro- and pre-B-cells and moderately in mature T-cells. Isoform 2 is highly expressed in testis and ovary and at a lower level in thymocytes, brain and kidney.
  • 数据库链接：

    KEGG: mmu:18753

    STRING: 10090.ENSMUSP00000107827

    UniGene: Mm.2314