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  4. Recombinant Mouse Protein phosphatase 1D (Ppm1d)

Recombinant Mouse Protein phosphatase 1D (Ppm1d)

  • 中文名称：
    小鼠Ppm1d重组蛋白
  • 货号：
    CSB-YP886398MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ppm1d重组蛋白
  • 货号：
    CSB-EP886398MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ppm1d重组蛋白
  • 货号：
    CSB-EP886398MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ppm1d重组蛋白
  • 货号：
    CSB-BP886398MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ppm1d重组蛋白
  • 货号：
    CSB-MP886398MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppm1d; Wip1; Protein phosphatase 1D; EC 3.1.3.16; Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-598
  • 氨基酸序列
    MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPAAEDKPA PVPRRALGLP ATPTLAGVGP SEKGPAAARD PAPDAAASLP AGRCCRRRSS VAFFAVCDGH GGREAAQFAR EHLWGFIKKQ KGFTSSEPAK VCAAIRKGFL ACHLAMWKKL AEWPKTMTGL PSTSGTTASV VIIRGMKMYV AHVGDSGVVL GIQDDPKDDF VRAVEVTQDH KPELPKERER IEGLGGSVMN KSGVNRVVWK RPRLTHSGPV RRSTVIDQIP FLAVARALGD LWSYDFFSGK FVVSPEPDTS VHTLDPRKHK YIILGSDGLW NMVPPQDAIS MCQDQEEKKY LMGEQGQSCA KMLVNRALGR WRQRMLRADN TSAIVICISP EVDNQGNFTN EDELFLNLTD SPTYNSQETC VMTSSPSSTP PIKSPEEDAW PRLSSKDHIP ALVRSNAFSE KFLEVPAEIA RGNIQTVVMT SKDSETLEEN CPKALTLRIH DSLNNTLSVG LIPTNSTNTI MDQKNLKMST PGQMKAQEVE RTPPANFKRT LEESNSGPLM KKHRRNGLSR SSGAQASSLP TASQRRHSVK LTLRRRLRGQ RKMGNPLLHQ HRKTVCVC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
  • 基因功能参考文献：
    1. Wip1 modulates macrophage migration and phagocytosis via Rac1-GTPase and PI3K/AKT signalling pathways. PMID: 28822916
    2. The results suggest that Wip1 could protect the heart from MI-induced ischemic injury. PMID: 28524834
    3. These data collectively indicate that Wip1 modulates host sensitivity to colitis by intrinsically regulating immune cells. PMID: 26950306
    4. Wip1 phosphatase plays a vital role in regulating hippocampal synaptic plasticity by modulating the phosphorylation of CaMKII. PMID: 27158969
    5. WIP1 phosphatase plays a pro-adipogenic role by interacting directly with PPARgamma and dephosphorylating p-PPARgamma S112 in vitro and in vivo. PMID: 28180926
    6. this study shows that knock out of Wip1 in mouse aggravates colonic inflammation and increases neutrophils migration PMID: 27687530
    7. the data indicate that the WIP1 phosphatase functions to maintain insulin sensitivity and glucose homeostasis. PMID: 25379953
    8. Study suggests a potential protective function of p53-induced phosphatase 1 in mood stabilization. PMID: 25732137
    9. our findings identify Wip1 as an intrinsic negative regulator of neutrophil inflammation in intestinal I/R injury process PMID: 25601473
    10. the principal function of Wip1 in MSCs transformation is the maintenance of proliferative capacity; but knocking out Wip1 increases the migratory capacity of MSCs; this dual effect of Wip1 provides the potential for purposeful routing of MSCs PMID: 25839408
    11. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy. PMID: 25704606
    12. We identified Wip1 as a potentiator of prolactin and HER2/neu signaling strictly in the molecular context of hormone-sensing cells. PMID: 23369183
    13. Wip1 prevents the induction of cellular senescence at physiological oxygen levels by attenuating DDR signaling in response to endogenous double-stranded breaks that form during DNA replication. PMID: 24552809
    14. the absence of Wip1 blocked radiation-induced intestinal tumorigenesis irrespective of radiation quality. PMID: 25117622
    15. augmenting WIP1 expression in aged animals markedly improved neuron formation and rescued a functional defect in fine odor discrimination in aged mice PMID: 24911145
    16. Expression of Wip1 in neutrophils was downregulated quickly after neutrophil activation through JNK-microRNA-16 pathway. Wip1-deficient mice had increased bactericidal activitiesand were hypersensitive to LPS-induced acute lung damage. PMID: 24395919
    17. Wip1 plays an important role in the regulation of global heterochromatin silencing and thus is critical in maintaining genome integrity PMID: 24135283
    18. Wip1 positively controls Thymic epithelial cells maturation, homeostasis, and regeneration PMID: 23966632
    19. Stable expression of Wip1 gene in chondrocytes attenuated the onset of senescence and dedifferentiation during in vitro expansion, resulting in the retention of a chondrocyte phenotype during prolonged culture. PMID: 23306038
    20. Our findings identify a previously unrecognized p53-independent function of Wip1 as a cell type-specific negative regulator of neutrophil generation and homeostasis through limiting the p38 MAPK-STAT1 pathway. PMID: 23212517
    21. A novel contribution of wip1 to cognition and dendritic spine morphology PMID: 22983193
    22. Wip1 phosphatase, a known negative regulator of Atm-dependent signaling, plays a major role in controlling fat accumulation and atherosclerosis in mice. PMID: 22768840
    23. The tumor-resistant phenotype of Wip1-deficient mice provides a model for Wip1's role in tumorigenesis. Wip1 also regulates neurogenesis from adult stem cells. Review. PMID: 22340722
    24. removing Wip1 from an Atm null mouse background reduced tumorigenesis, enhanced longevity, augmented the DNA damage response, decreased genomic instability and partially rescued gametogenesis. PMID: 21765465
    25. WIP1 may suppress nucleotide excision repair (NER) kinetics by dephosphorylating and inactivating XPA and XPC and other NER proteins and regulators after UV-induced DNA damage is repaired. PMID: 20451471
    26. Nuclear factor-kappaB (NF-kappaB) is a novel positive transcriptional regulator of the oncogenic Wip1 phosphatase PMID: 20007970
    27. inactivation or depletion of the Wip1 phosphatase with resultant p38 MAPK activation suppresses tumor appearance by modulating the Cdkn2a tumor-suppressor locus PMID: 14991053
    28. Data demonstrate that p53-induced phosphatase PPM1D interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. PMID: 15327777
    29. Wip1 down-regulates p53 activation in the thymus and is required for normal alphabeta T cell development. PMID: 16585576
    30. PPM1D has a homeostatic role in abrogating the DNA damage response and may regulate aspects of male longevity. PMID: 16652371
    31. Wip1 phosphatase is an integral component of an ATM-dependent signaling pathway. PMID: 16949371
    32. Wip1 phosphatase is critical for regulating the ATM-mediated tumor surveillance network. PMID: 17158963
    33. WIP1 is a direct phosphatase of Ser 536 of the p65 subunit of NF-kappaB and negatively regulates NF-kappaB signalling. PMID: 19377466
    34. Loss of Wip1 sensitizes cells to stress- and DNA damage-induced apoptosis. PMID: 19395378

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    PP2C family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    KEGG: mmu:53892

    STRING: 10090.ENSMUSP00000020835

    UniGene: Mm.45609